[Effect of microwave radiation on primary cultured Sertoli cells].

AI: Melanie Research RF Safe Research Library Jan 1, 2009 CONCERN MEDIUM

This in vitro study exposed primary cultured Sertoli cells to microwave radiation at 0, 30, or 100 mW/cm^2 for 5 minutes and assessed outcomes 6 hours later. The authors report no differences in apoptosis/death or intracellular Ca2+ at 30 mW/cm^2, though cell-cycle distribution shifted. At 100 mW/cm^2, intracellular Ca2+ increased and apoptosis/death increased, with cell-cycle changes consistent with growth inhibition.

Key points

Primary cultured Sertoli cells were exposed to microwave radiation for 5 minutes at 0, 30, or 100 mW/cm^2.
Cell cycle, apoptosis/death, and intracellular Ca2+ were measured 6 hours after exposure using flow cytometry and confocal microscopy methods.
At 30 mW/cm^2, apoptosis/death and intracellular Ca2+ did not differ from controls, but cell-cycle distribution shifted toward S phase.
At 100 mW/cm^2, intracellular Ca2+ increased and apoptosis/death increased compared with controls.
At 100 mW/cm^2, cell-cycle distribution shifted toward G0-G1 with reduced S and G2-M phases, interpreted as growth inhibition.
The authors propose increased intracellular Ca2+ as a potential mechanism of injury under the higher exposure condition.

Referenced studies & papers

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