Continuous exposure to 60 Hz extremely low frequency magnetic field at 10-14 mT promotes various

Abstract

Continuous exposure to 60 Hz extremely low frequency magnetic field at 10-14 mT promotes various human cell proliferation by activating extracellular-signal-regulated kinase Goh J, Suh D, Um DY, Chae SA, Park GS, Song K. Continuous exposure to 60 Hz extremely low frequency magnetic field at 10-14 mT promotes various human cell proliferation by activating extracellular-signal- regulated kinase. Biochem Biophys Res Commun. 2025 Jan 28;751:151414. doi: 10.1016/j.bbrc.2025.151414. Abstract We previously showed that 60 Hz extremely low-frequency magnetic fields (ELF-MF) at 6 mT promote various humancell proliferation. This study investigated the effects of 60 Hz ELF-MF at 10-16 mT on various mammalian cells, including human cervical carcinoma, rat neuroblastoma, liver cancer stem cells, immortalized normal hepatic cells, and normal fibroblasts. Using a revised ELF-MF-generating device that increases magnetic flux density stably without thermal effects, we exposed cells to 10 and 16 mT ELF-MF for 72 h. All cell types exhibited an approximately 20 % or greater increase in proliferation compared to the sham exposure group at 14 mT, with no further increase observed at 16 mT. In cells with activated proliferation at 14 mT, we observed activation of the MEK-ERK pathway and NF-κB, but not Akt, and a slight increase in S phase population. Intracellular and mitochondrial ROS levels remained unchanged, and the proliferation-activating effects persisted when oxidative phosphorylation was interrupted. No changes in intracellular calcium levels were observed, and the proliferation-activating effects were maintained in the presence of a calcium chelator. These findings suggest that ROS and intracellular calcium do not mediate ELF-MF's proliferation-activating physiological effect. In conclusion, exposure to 60 Hz ELF-MF at 10-14 mT promotes cell proliferation by activating ERK1/2 without affecting intracellular ROS and calcium levels. pubmed.ncbi.nlm.nih.gov

AI evidence extraction

Main findings

Using a device designed to increase magnetic flux density without thermal effects, cells exposed to 60 Hz ELF-MF at 10 and 16 mT for 72 h showed increased proliferation at 14 mT (approximately ≥20% vs sham) across multiple mammalian cell types, with no further increase at 16 mT. In cells with increased proliferation at 14 mT, MEK-ERK and NF-κB were activated (Akt was not), with a slight increase in S phase; intracellular/mitochondrial ROS and intracellular calcium were unchanged, and the proliferation effect persisted despite interruption of oxidative phosphorylation and in the presence of a calcium chelator.

Outcomes measured

• Cell proliferation
• MEK-ERK (ERK1/2) pathway activation
• NF-κB activation
• Akt signaling
• Cell cycle (S phase population)
• Intracellular ROS
• Mitochondrial ROS
• Oxidative phosphorylation dependence
• Intracellular calcium levels

Limitations

• In vitro study (cell culture), limiting direct inference to human health outcomes
• Sample sizes and replication details not provided in the abstract
• Exposure-response details for 10 mT and 16 mT effects are not fully described in the abstract

Suggested hubs

• elF-cell-studies (0.9)
  In vitro study of 60 Hz ELF magnetic field effects on mammalian/human cell proliferation and signaling.

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    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.059999999999999997779553950749686919152736663818359375,
        "sar_wkg": null,
        "duration": "72 h continuous"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Cell proliferation",
        "MEK-ERK (ERK1/2) pathway activation",
        "NF-κB activation",
        "Akt signaling",
        "Cell cycle (S phase population)",
        "Intracellular ROS",
        "Mitochondrial ROS",
        "Oxidative phosphorylation dependence",
        "Intracellular calcium levels"
    ],
    "main_findings": "Using a device designed to increase magnetic flux density without thermal effects, cells exposed to 60 Hz ELF-MF at 10 and 16 mT for 72 h showed increased proliferation at 14 mT (approximately ≥20% vs sham) across multiple mammalian cell types, with no further increase at 16 mT. In cells with increased proliferation at 14 mT, MEK-ERK and NF-κB were activated (Akt was not), with a slight increase in S phase; intracellular/mitochondrial ROS and intracellular calcium were unchanged, and the proliferation effect persisted despite interruption of oxidative phosphorylation and in the presence of a calcium chelator.",
    "effect_direction": "benefit",
    "limitations": [
        "In vitro study (cell culture), limiting direct inference to human health outcomes",
        "Sample sizes and replication details not provided in the abstract",
        "Exposure-response details for 10 mT and 16 mT effects are not fully described in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "60 Hz",
        "extremely low frequency magnetic field",
        "ELF-MF",
        "10-16 mT",
        "14 mT",
        "cell proliferation",
        "ERK1/2",
        "MEK-ERK pathway",
        "NF-κB",
        "ROS",
        "intracellular calcium",
        "sham exposure"
    ],
    "suggested_hubs": [
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}

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