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4 postsFilters: tag: in-vitro Clear
Differential metabolic responses of mouse Leydig and spermatogonia cells to radiofrequency electromagnetic field exposure
This in vitro study used LC-MS metabolomics to assess how continuous versus intermittent RF-EMF irradiation affects mouse Leydig (TM3) and spermatogonia (GC-1) cells. The authors report stronger metabolic disturbances in TM3 cells under continuous exposure, including changes in amino acid and glutathione-related pathways, while intermittent exposure mainly affected fatty acyl and purine-related metabolism. GC-1 cells were reported to be less sensitive, and ADP changes were proposed as a potential metabolic signature. The authors interpret these metabolic disturbances as suggesting potential reproductive health risks.
Impact of in vitro exposure to 5G-modulated 3.5 GHz fields on oxidative stress and DNA repair in skin cells
This in vitro study tested whether 5G-modulated 3.5 GHz RF-EMF exposure affects oxidative stress and DNA repair in human skin cells. Under acute exposure conditions (up to 24–48h) at SARs up to 4 W/kg, the authors report no significant changes in ROS markers, no adaptive response to oxidative challenge, and no impairment of UV-B–related CPD repair via nucleotide excision repair. The authors note that acute in vitro results may not directly generalize to chronic or real-life exposures.
Exposure to radiofrequency electromagnetic fields and IARC carcinogen assessment: Risk of Bias preliminary literature assessment for 10 key characteristics of human carcinogens
This review examined experimental literature on whether RF-EMF exposures within ICNIRP (2020) limits affect IARC key characteristics of human carcinogens. It identified 159 articles and found that 38% of in vitro/in vivo measurements reported statistically significant effects, but higher study quality was associated with fewer reported effects and there was no consistent exposure-response pattern. The authors state that study diversity and generally poor quality prevent high-confidence conclusions for most key characteristics, while recommending replication of the few higher-quality positive findings under stringent standards.
Mitigation of 3.5 GHz Electromagnetic Field-Induced BV2 Microglial Cytotoxicity by Polydeoxyribonucleotide
This in vitro study exposed BV2 mouse microglial cells to 3.5 GHz EMF for 2 hours and reports reduced cell growth and increased apoptosis alongside oxidative stress and signaling changes. The authors report that ROS generation and activation of JNK-1/2 and p38 MAPK were key events in the observed cytotoxicity. Polydeoxyribonucleotide (PDRN) reportedly reduced several EMF-associated cytotoxicity markers, suggesting a potential mitigating effect under the tested conditions.