Assessment of protein and DNA polymorphisms in corn (Zea mays) under the effect of non-ionizing electromagnetic radiation

PAPER manual 2023 Animal study Effect: harm Evidence: Low

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Abstract

Assessment of protein and DNA polymorphisms in corn (Zea mays) under the effect of non-ionizing electromagnetic radiation Abdelhaliem E, Abdalla HM, Bolbol AA, Shehata RS. Assessment of protein and DNA polymorphisms in corn (Zea mays) under the effect of non-ionizing electromagnetic radiation. Caryologia [Internet]. 2023. 75(4). Abstract Many reports highlight biological responses of crop plants after non-ionizing electromagnetic radiation (EMR) exposure based on the phenotypic and physiological levels. So, this study aimed to estimate genetic alterations in proteins, isozymes, and DNA banding patterns as well as the extent of nuclear DNA damage of economic corn (Zea mays) under the stress of EMR using accurate and reliable bioassays like sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE), isozymes (Leucine- aminopeptidase, Esterases, Peroxidase, and Catalases), random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR), and Comet Assay, respectively. SDS-PAGE analysis showed distinct polymorphisms (96.66%) between EMR exposed and non- exposed corn seedlings depending on the number and type of bands, their intensities as well as molecular weight which ranged from (60.27 to 192.35 kDa), gain, and loss of bands. The four isozymes generated varies isozymatic polymorphisms based on relative front, zymogram number, and optical intensities. RAPD analysis generated 85 amplified DNA products with high polymorphism values ranged from 90.91 to 100% based on primers, band type, DNA sizes which ranged from 153 to 1008-bp, lose, gain, and intensity of DNA bands. Comet Assay scored highest extent of loosed DNA from nuclei (DNA damage) reached the value of (tailed ratio 20%) at EMR exposed corn nuclei for 5 days compared to non-exposed nuclei which reached the value of (tailed ratio 3%). This study concluded that each EMR exposure time had unique interaction with proteins, isozymes, and DNA of corn cells exhibiting wide range of genotoxic stress and subsequently, adversely effect on growth and yield of this sensitive crop plants. Open access paper: riviste.fupress.net

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Corn (Zea mays) seedlings/cells

Sample size

—

Exposure

non-ionizing · up to 5 days (exposure time varied; 5 days reported for comet assay)

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

EMR-exposed corn showed distinct protein polymorphisms by SDS-PAGE (reported 96.66%) and isozyme pattern changes across four enzyme systems. RAPD-PCR produced 85 DNA products with high polymorphism (90.91–100% depending on primer), with band gain/loss and intensity changes. Comet assay indicated higher nuclear DNA damage in exposed nuclei (tailed ratio 20% at 5 days) versus non-exposed (tailed ratio 3%).

Outcomes measured

Protein banding polymorphisms (SDS-PAGE)
Isozyme polymorphisms (leucine-aminopeptidase, esterases, peroxidase, catalases)
DNA banding polymorphisms (RAPD-PCR)
Nuclear DNA damage (Comet assay; tailed ratio)
Growth and yield (mentioned as adversely affected in conclusion)

Limitations

EMR exposure characteristics (e.g., frequency, field strength, modulation) not reported in the provided abstract/metadata
Sample size and experimental replication not reported
Quantitative growth/yield results not provided in the abstract

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "non-ionizing",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "up to 5 days (exposure time varied; 5 days reported for comet assay)"
    },
    "population": "Corn (Zea mays) seedlings/cells",
    "sample_size": null,
    "outcomes": [
        "Protein banding polymorphisms (SDS-PAGE)",
        "Isozyme polymorphisms (leucine-aminopeptidase, esterases, peroxidase, catalases)",
        "DNA banding polymorphisms (RAPD-PCR)",
        "Nuclear DNA damage (Comet assay; tailed ratio)",
        "Growth and yield (mentioned as adversely affected in conclusion)"
    ],
    "main_findings": "EMR-exposed corn showed distinct protein polymorphisms by SDS-PAGE (reported 96.66%) and isozyme pattern changes across four enzyme systems. RAPD-PCR produced 85 DNA products with high polymorphism (90.91–100% depending on primer), with band gain/loss and intensity changes. Comet assay indicated higher nuclear DNA damage in exposed nuclei (tailed ratio 20% at 5 days) versus non-exposed (tailed ratio 3%).",
    "effect_direction": "harm",
    "limitations": [
        "EMR exposure characteristics (e.g., frequency, field strength, modulation) not reported in the provided abstract/metadata",
        "Sample size and experimental replication not reported",
        "Quantitative growth/yield results not provided in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "non-ionizing electromagnetic radiation",
        "EMR",
        "Zea mays",
        "corn",
        "genotoxicity",
        "DNA damage",
        "comet assay",
        "RAPD-PCR",
        "SDS-PAGE",
        "isozymes"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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