[Microwave radiation decreases the expressions of occludin and JAM-1 in rats].

Abstract

OBJECTIVE: To explore the changes in the expressions of the tight junction related protein occludin and junctional adhesion molecule-1 (JAM-1) of the blood-testis barrier and their significance in rats after microwave radiation. METHODS: Eighty male Wistar rats were exposed to microwave radiation with average power density of 0, 10, 30 and 100 mW/cm2 for five minutes, and dynamic changes in the expressions of testicular occludin and JAM-1 were observed by Western blot and image analysis at 6 h, 1 d, 3 d, 7 d and 14 d after the radiation. RESULTS: There was a significant down-regulation in the expression of the occludin protein at 3 - 7 d, 6 h - 7 d and 6 h - 14 d (P < 0. 05), as well as in that of JAM-1 at 3 - 7 d, 1 - 7 d and 1-14 d (P < 0.05) after exposure to 10, 30 and 100 mW/cm2 microwave radiation. CONCLUSION: The decreased protein expressions of occludin and JAM-1 may play an important role in the microwave radiation induced-damage to the blood-testis barrier.

AI evidence extraction

Main findings

Rats exposed to microwave radiation at 10, 30, or 100 mW/cm2 for 5 minutes showed significant down-regulation of testicular occludin and JAM-1 protein expression at multiple post-exposure time points (ranging from 6 hours up to 14 days, depending on dose and protein) compared with 0 mW/cm2.

Outcomes measured

• Testicular occludin protein expression
• Testicular JAM-1 (junctional adhesion molecule-1) protein expression
• Blood-testis barrier (tight junction-related proteins)

Limitations

• Microwave frequency not reported in abstract
• No SAR or dosimetry beyond average power density reported in abstract
• Outcome limited to protein expression markers; functional blood-testis barrier integrity not directly reported in abstract

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "5 minutes"
    },
    "population": "Male Wistar rats",
    "sample_size": 80,
    "outcomes": [
        "Testicular occludin protein expression",
        "Testicular JAM-1 (junctional adhesion molecule-1) protein expression",
        "Blood-testis barrier (tight junction-related proteins)"
    ],
    "main_findings": "Rats exposed to microwave radiation at 10, 30, or 100 mW/cm2 for 5 minutes showed significant down-regulation of testicular occludin and JAM-1 protein expression at multiple post-exposure time points (ranging from 6 hours up to 14 days, depending on dose and protein) compared with 0 mW/cm2.",
    "effect_direction": "harm",
    "limitations": [
        "Microwave frequency not reported in abstract",
        "No SAR or dosimetry beyond average power density reported in abstract",
        "Outcome limited to protein expression markers; functional blood-testis barrier integrity not directly reported in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "rats",
        "Wistar",
        "blood-testis barrier",
        "tight junction",
        "occludin",
        "JAM-1",
        "testis",
        "power density",
        "Western blot"
    ],
    "suggested_hubs": []
}

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