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Ameliorative Role of Coenzyme Q10 in RF Radiation-Associated Testicular and Oxidative Impairments in a 3.5-GHz Exposure Model

Research RF Safe Research Library Jan 1, 2026

This animal experiment assessed GSM-modulated 3.5 GHz RF exposure in male Wistar rats and reported hormonal, oxidative, and histological changes consistent with testicular impairment. RF exposure was associated with lower testosterone, LH, and FSH, higher oxidative stress (increased MDA and TOS), and degenerative testicular histology. Coenzyme Q10 supplementation partially mitigated several reported changes. The authors caution against generalizing these results to FR1 5G NR signals and call for further research.

Oxidative stress and testicular damage induced by chronic exposure to 35.5 GHz millimeter wave radiation in male Wistar rats

Research RF Safe Research Library Jan 1, 2025

This randomized controlled animal study examined chronic 35.5 GHz millimeter wave exposure in male Wistar rats (2 hours/day for 60 days) compared with control and sham groups. The exposed group showed reduced sperm count and viability along with testicular histopathological changes. Oxidative stress markers shifted toward increased lipid peroxidation and reduced antioxidant defenses, and comet assay results indicated increased DNA damage.

Histomorphometric study of thyroid tissue in juvenile rats exposed to 5G electromagnetic fields

Research RF Safe Research Library Jan 1, 2025

This animal study examined thyroid histomorphometry in juvenile male Wistar rats after 2 weeks of 5G EMF exposure (3.5 GHz, 1.5 V/m). Exposed rats showed larger follicle and colloid areas and a significantly lower Thyroid Activation Index, which the authors interpret as thyroid hypoactivity. The authors suggest this may represent a potential health risk and call for further work including hormone assays and mechanistic studies.

Impairment of Oogenesis and Folliculogenesis in Neonatal Rats after Maternal Exposure to Mobile Phones

Research RF Safe Research Library Jan 1, 2025

This animal study examined maternal mobile phone exposure during different gestational windows in Wistar rats and assessed ovarian development and hormones in neonatal offspring. Compared with sham (phone off), exposed groups (standby and conversation/standby) were reported to have lower neonatal estrogen and progesterone and reduced primordial follicle/primary oocyte measures, with stronger effects after longer exposure. The study also reports increased primordial follicle apoptosis, particularly in the conversation/standby condition, and notes effects even with first-week gestational exposure.

Effect of short-term extremely low-frequency electromagnetic field on respiratory functions

Research RF Safe Research Library Jan 1, 2025

This animal study tested whether short-term ELF-EMF exposure alters respiratory physiology in rats. Twenty Wistar albino rats were assigned to control or EMF exposure (50 Hz, 0.3 mT for 2 minutes) with respiratory parameters measured before, during, and after exposure. The study reports changes during exposure (lower respiratory rate and higher cycle duration, inspiration time, and tidal volume) but no differences after exposure, and it frames the findings as relevant to EMF safety and potential health risks.

Effects of 700MHz radiofrequency radiation (5G lower band) on the reproductive parameters of female Wistar rats

Research RF Safe Research Library Jan 1, 2025

This animal study examined short- and long-term 700 MHz (lower-band 5G) radiofrequency exposure in female Wistar rats, comparing control, sham, and exposed groups. It reports no DNA damage and no change in estrous cycle length, but increased ovarian oxidative stress markers in exposed animals. Long-term exposure was associated with ovarian histopathological alterations, while estradiol and progesterone stayed within normal ranges and testosterone increased slightly but significantly.

Possible effects of RF EMR on contextual fear conditioning, hippocampal perivascular space, apoptosis and adrenal gland microarchitecture in rats

Research RF Safe Research Library Jan 1, 2025

This animal study exposed male Wistar rats to 900 MHz RF-EMR from a mobile phone for 1 hour daily over four weeks and assessed behavior and tissue changes. The authors report altered contextual fear conditioning-related behavior in exposed rats. Histological assessments indicated apoptosis and enlarged perivascular space in the hippocampal CA3 region and apoptotic/inflammatory-like changes in the adrenal zona fasciculata, with no reported differences in adrenal medulla cytoarchitecture.

5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis

Research RF Safe Research Library Jan 1, 2025

This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while UCP1-dependent thermogenesis markers were not significantly changed at the transcriptional level. The authors interpret these findings as a potential partial disruption of brown adipocyte differentiation and raise EMF safety concerns, while calling for further confirmatory research.

Experimental Study of Animal Behavior under the Influence of the Electromagnetic Field of the 5G Mobile Communication Standard

Research RF Safe Research Library Jan 1, 2024

This pilot animal study exposed mature male Wistar rats to 4.9 GHz electromagnetic fields described as 5G-standard, for 15 days (2 hours/day) in a semi-anechoic shielded chamber. Open-field testing found no clear behavioral differences between unmodulated and modulated exposures. Statistically significant behavioral changes occurred in both exposed and control animals, which the authors attribute to changes in the environmental electromagnetic background, making causal interpretation uncertain.

Effect of Repeated Exposure to Complexly Organized Electromagnetic Radiation on the Rat Behavior in the "Open Field" Test

Research RF Safe Research Library Jan 1, 2024

This animal study examined repeated pulsed-modulated RF exposure (1–4 GHz; total pulse power density 300 μW/cm2) in male and female Wistar rats and assessed behavior using the open field test. The abstract reports stress reactions and long-term memory impairment in some rats, with females described as more sensitive than males. Reported effects were transient, with behavior returning to baseline within 1.5–2 months after exposure stopped. The authors suggest potential concern for constant exposure scenarios, though this is not directly evaluated in humans here.

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