Induction of apoptosis in B16-BL6 melanoma cells following exposure to electromagnetic fields modeled after intercellular calcium waves.

Abstract

Exposure to time-varying electromagnetic fields (EMF) has the capacity to influence biological systems. Our results demonstrate that exposure to time-varying EMF modeled after the physiological firing frequency of intercellular calcium waves can inhibit proliferation and induce apoptosis in malignant cells. Single exposure of B16-BL6 cells to a Ca EMF for 40 min reduced the number of viable cells by 50.3%. Cell imaging with acridine orange and ethidium bromide dye revealed substantial cellular apoptosis, preapoptotic cells, nuclear fragmentation, and large spacing between cells in the Ca EMF condition when compared to the control condition. The ability of Ca EMF to influence the proliferation and survival of malignant cells suggests that exposure to specific EMF may function as a potential anticancer therapy.

AI evidence extraction

Main findings

A single 40 min exposure of B16-BL6 melanoma cells to a time-varying EMF modeled after intercellular calcium wave firing frequency reduced viable cell number by 50.3% and was associated with imaging evidence of substantial apoptosis and nuclear fragmentation compared with control.

Outcomes measured

• Cell proliferation
• Cell viability
• Apoptosis (including preapoptotic cells, nuclear fragmentation)
• Cell morphology/spacing (imaging findings)

Limitations

• Frequency and other exposure parameters (e.g., field strength, SAR) not reported in the abstract
• In vitro cell-line study; generalizability to organisms/clinical outcomes not addressed in the abstract
• Sample size and replication details not provided in the abstract

{
    "study_type": "in_vitro",
    "exposure": {
        "band": null,
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "Single exposure for 40 min"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Cell proliferation",
        "Cell viability",
        "Apoptosis (including preapoptotic cells, nuclear fragmentation)",
        "Cell morphology/spacing (imaging findings)"
    ],
    "main_findings": "A single 40 min exposure of B16-BL6 melanoma cells to a time-varying EMF modeled after intercellular calcium wave firing frequency reduced viable cell number by 50.3% and was associated with imaging evidence of substantial apoptosis and nuclear fragmentation compared with control.",
    "effect_direction": "benefit",
    "limitations": [
        "Frequency and other exposure parameters (e.g., field strength, SAR) not reported in the abstract",
        "In vitro cell-line study; generalizability to organisms/clinical outcomes not addressed in the abstract",
        "Sample size and replication details not provided in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "time-varying electromagnetic fields",
        "intercellular calcium waves",
        "B16-BL6",
        "melanoma cells",
        "apoptosis",
        "cell viability",
        "proliferation",
        "anticancer therapy"
    ],
    "suggested_hubs": []
}

Comments

Log in to comment.

No comments yet.