The Time-Dependence of Three Different Modes of ELF-EMF Stimulation on LTP at Schaffer Collateral-CA1 Synapses.

PAPER pubmed Bioelectromagnetics 2021 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.1002/bem.22369 PMID: 34449888 Evidence Lab

Abstract

Long-term potentiation (LTP) is considered the cellular basis of learning and memory. Extremely low-frequency electromagnetic fields (ELF-EMFs) are neuromodulation tools for regulating LTP. However, the temporal effects of short-term ELF-EMF stimulation on LTP are not yet known. In this study, we evaluated the time-dependent effects of 15 Hz/2 mT ELF-EMF stimulation on LTP at the Schaffer collateral-CA1 (SC-CA1) synapses in Sprague-Dawley rats. Hippocampal slices were exposed to three different modes of ELF-EMFs (sinusoidal, single-frequency pulse, and rhythm pulse) and durations (10, 20, 40, and 60 s). The baseline was recorded for 20 min and field excitatory postsynaptic potential (fEPSP) was recorded for 60 min using multi-electrode arrays (MEA) after plasticity induction using 100 Hz electrical high-frequency stimulation (HFS). Compared to the control group, the LTP decreased under three different magnetic fields and was proportional to time; that is, the longer the time, the greater the inhibition. We also compared the three magnetic fields and showed that the continuous sinusoidal magnetic field had the largest inhibitory rate of LTP, while pulsed and rhythm pulsed magnetic fields were similar. We showed that different modes of ELF-EMF stimulation had a time-dependent effect on LTP at Schaffer collateral-CA1 synapses, which provides experimental evidence for the treatment of related neurological diseases. © 2021 Bioelectromagnetics Society.

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Sprague-Dawley rats (hippocampal slices; SC-CA1 synapses)

Sample size

—

Exposure

ELF other · 10, 20, 40, and 60 s

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Hippocampal slices exposed to 15 Hz/2 mT ELF-EMF showed decreased LTP compared with controls across sinusoidal, single-frequency pulse, and rhythm pulse modes. The inhibition of LTP increased with longer exposure duration, and continuous sinusoidal stimulation produced the largest inhibitory effect, while pulsed and rhythm-pulsed modes were similar.

Outcomes measured

Long-term potentiation (LTP) at Schaffer collateral-CA1 synapses
Field excitatory postsynaptic potential (fEPSP) recordings after high-frequency stimulation (HFS)

Limitations

Sample size not reported in the provided abstract/metadata
Ex vivo hippocampal slice preparation; findings may not directly translate to in vivo learning/memory outcomes
Short-term exposure durations only (10–60 s)

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "10, 20, 40, and 60 s"
    },
    "population": "Sprague-Dawley rats (hippocampal slices; SC-CA1 synapses)",
    "sample_size": null,
    "outcomes": [
        "Long-term potentiation (LTP) at Schaffer collateral-CA1 synapses",
        "Field excitatory postsynaptic potential (fEPSP) recordings after high-frequency stimulation (HFS)"
    ],
    "main_findings": "Hippocampal slices exposed to 15 Hz/2 mT ELF-EMF showed decreased LTP compared with controls across sinusoidal, single-frequency pulse, and rhythm pulse modes. The inhibition of LTP increased with longer exposure duration, and continuous sinusoidal stimulation produced the largest inhibitory effect, while pulsed and rhythm-pulsed modes were similar.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in the provided abstract/metadata",
        "Ex vivo hippocampal slice preparation; findings may not directly translate to in vivo learning/memory outcomes",
        "Short-term exposure durations only (10–60 s)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "15 Hz",
        "2 mT",
        "long-term potentiation",
        "LTP",
        "Schaffer collateral",
        "CA1",
        "hippocampus",
        "Sprague-Dawley rat",
        "hippocampal slices",
        "fEPSP",
        "multi-electrode array",
        "sinusoidal",
        "pulsed",
        "rhythm pulse"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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