Effect of extremely low frequency (ELF) magnetic field exposure on morphological and biophysical properties of human lymphoid cell line (Raji).

Abstract

Human B lymphoid cells (Raji) were exposed for 72 h to a 50 Hz sinusoidal magnetic field at a density of 2 milliTesla (rms). The results of exposure showed a decrease in membrane fluidity as detected by Laurdan emission spectroscopy and DPH fluorescence polarization. Field exposure also resulted in a reorganization of cytoskeletal components. Scanning electron microscopy (SEM) revealed a loss of microvilli in the exposed cells. This change in plasma membrane morphology was accompanied by a different actin distribution, as detected by phalloidin fluorescence. We also present evidence that EMF exposure of Raji cells can interfere with protein phosphorylation. Our observations confirm the hypothesis that electric and magnetic fields may modify the plasma membrane structure and interfere with the initiation of the signal cascade pathways.

AI evidence extraction

Main findings

Raji cells exposed for 72 h to a 50 Hz, 2 mT (rms) sinusoidal magnetic field showed decreased membrane fluidity, reorganization of cytoskeletal components, and loss of microvilli on SEM with altered actin distribution. The authors also report evidence that exposure can interfere with protein phosphorylation.

Outcomes measured

• Membrane fluidity (Laurdan emission spectroscopy; DPH fluorescence polarization)
• Cytoskeletal organization
• Plasma membrane morphology (microvilli; SEM)
• Actin distribution (phalloidin fluorescence)
• Protein phosphorylation

Limitations

• In vitro cell line model; findings may not generalize to in vivo human health outcomes
• Sample size not reported in abstract
• Exposure conditions limited to 50 Hz, 2 mT, 72 h

Suggested hubs

• occupational-exposure (0.2)
  Study involves ELF magnetic field exposure (50 Hz), relevant to some occupational ELF contexts, though this is an in vitro model.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "72 h"
    },
    "population": "Human B lymphoid cell line (Raji)",
    "sample_size": null,
    "outcomes": [
        "Membrane fluidity (Laurdan emission spectroscopy; DPH fluorescence polarization)",
        "Cytoskeletal organization",
        "Plasma membrane morphology (microvilli; SEM)",
        "Actin distribution (phalloidin fluorescence)",
        "Protein phosphorylation"
    ],
    "main_findings": "Raji cells exposed for 72 h to a 50 Hz, 2 mT (rms) sinusoidal magnetic field showed decreased membrane fluidity, reorganization of cytoskeletal components, and loss of microvilli on SEM with altered actin distribution. The authors also report evidence that exposure can interfere with protein phosphorylation.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell line model; findings may not generalize to in vivo human health outcomes",
        "Sample size not reported in abstract",
        "Exposure conditions limited to 50 Hz, 2 mT, 72 h"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "ELF",
        "magnetic field",
        "50 Hz",
        "2 mT",
        "Raji cells",
        "B lymphoid cell line",
        "membrane fluidity",
        "Laurdan",
        "DPH fluorescence polarization",
        "cytoskeleton",
        "microvilli",
        "scanning electron microscopy",
        "actin",
        "phalloidin",
        "protein phosphorylation"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.200000000000000011102230246251565404236316680908203125,
            "reason": "Study involves ELF magnetic field exposure (50 Hz), relevant to some occupational ELF contexts, though this is an in vitro model."
        }
    ]
}

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