[Effect of low-intensity electromagnetic fields of industrial frequency on the ultrastructure and proliferative activity of rat's thymus cells].

Abstract

Effects of two types of low-intensity electromagnetic fields (EMF) of industrial frequency (50 Hz) on the fine structure and proliferative activity of thymic cells in white rats were studied. It was found that a weak EMF with a prevailing electrical component (380-480 V/m, 120-140 nT1) did not affect the DNA synthesis intensity. An EMF with a stronger magnetic induction (10-15 V/m, 800-1500 nT1) diminished the glucose-6-phosphate dehydrogenase activity and proliferative processes in cultured stimulated lymphocytes. Electron microscopic investigation of the thymus after both types of exposure revealed an accumulation of lymphocytes with pyknotic nuclei and electron-dense cytoplasm, as well as hypoplasia of the vascular endothelium. At the same time, EMF with a prevailing magnetic component produced a more marked negative effect on the ultrastructure of thymic cells, which indicated a lowered secretory activity of epitheliocytes.

AI evidence extraction

Main findings

Two low-intensity 50 Hz EMF conditions were studied. A weak EMF with prevailing electrical component (380–480 V/m, 120–140 nT) did not affect DNA synthesis intensity, while an EMF with stronger magnetic induction (10–15 V/m, 800–1500 nT) diminished glucose-6-phosphate dehydrogenase activity and proliferative processes in cultured stimulated lymphocytes. Electron microscopy after both exposures showed accumulation of lymphocytes with pyknotic nuclei and electron-dense cytoplasm and hypoplasia of vascular endothelium; effects were more marked with the prevailing magnetic component, suggesting lowered secretory activity of epitheliocytes.

Outcomes measured

• DNA synthesis intensity
• Glucose-6-phosphate dehydrogenase activity
• Proliferative processes in cultured stimulated lymphocytes
• Thymus cell ultrastructure (electron microscopy)
• Vascular endothelium hypoplasia
• Secretory activity of epitheliocytes

Limitations

• Sample size not reported in abstract
• Exposure duration and schedule not reported in abstract
• Details of experimental design and controls not reported in abstract
• Outcomes include both in vivo thymus ultrastructure and in vitro cultured lymphocyte measures, with limited methodological detail in abstract

Suggested hubs

• occupational-exposure (0.55)
  Exposure described as industrial-frequency (50 Hz) low-intensity EMF, relevant to occupational/industrial settings.

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": "industrial frequency",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "White rats (thymus cells; cultured stimulated lymphocytes)",
    "sample_size": null,
    "outcomes": [
        "DNA synthesis intensity",
        "Glucose-6-phosphate dehydrogenase activity",
        "Proliferative processes in cultured stimulated lymphocytes",
        "Thymus cell ultrastructure (electron microscopy)",
        "Vascular endothelium hypoplasia",
        "Secretory activity of epitheliocytes"
    ],
    "main_findings": "Two low-intensity 50 Hz EMF conditions were studied. A weak EMF with prevailing electrical component (380–480 V/m, 120–140 nT) did not affect DNA synthesis intensity, while an EMF with stronger magnetic induction (10–15 V/m, 800–1500 nT) diminished glucose-6-phosphate dehydrogenase activity and proliferative processes in cultured stimulated lymphocytes. Electron microscopy after both exposures showed accumulation of lymphocytes with pyknotic nuclei and electron-dense cytoplasm and hypoplasia of vascular endothelium; effects were more marked with the prevailing magnetic component, suggesting lowered secretory activity of epitheliocytes.",
    "effect_direction": "mixed",
    "limitations": [
        "Sample size not reported in abstract",
        "Exposure duration and schedule not reported in abstract",
        "Details of experimental design and controls not reported in abstract",
        "Outcomes include both in vivo thymus ultrastructure and in vitro cultured lymphocyte measures, with limited methodological detail in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "50 Hz",
        "industrial frequency",
        "rats",
        "thymus",
        "lymphocytes",
        "ultrastructure",
        "electron microscopy",
        "DNA synthesis",
        "glucose-6-phosphate dehydrogenase",
        "proliferation"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.5500000000000000444089209850062616169452667236328125,
            "reason": "Exposure described as industrial-frequency (50 Hz) low-intensity EMF, relevant to occupational/industrial settings."
        }
    ]
}

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