C-phycocyanin extraction assisted by pulsed electric field from Artrosphira platensis.

Abstract

This paper assesses the application of pulsed electric fields (PEF) to the fresh biomass of Artrhospira platensis in order to enhance the extraction of C-phycocyanin into aqueous media. Electroporation of A. platensis depended on both electric field strength and treatment duration. The minimum electric field intensity for detecting C-phycocyanin in the extraction medium was 15kV/cm after the application of a treatment time 150μs (50 pulses of 3μs). However higher electric field strength were required when shorter treatment times were applied. Response surface methodology was used in order to investigate the influence of electric field strength (15-25kV/cm), treatment time (60-150μs), and temperature of application of PEF (10-40°C) on C-phycocyanin extraction yield (PEY). The increment of the temperature PEF treatment reduced the electric field strength and the treatment time required to obtain a given PEY and, consequently decreased the total specific energy delivered by the treatment. For example, the increment of temperature from 10°C to 40°C permitted to reduce the electric field strength required to extract 100mg/g d of C-phycocyanin from 25 to 18kV/cm, and the specific energy input from 106.7 to 67.5kJ/Kg. Results obtained in this investigation demonstrated PEF's potential for selectively extraction C-phycocyanin from fresh A. platensis biomass. The purity of the C-phycocyanin extract obtained from the electroporated cells was higher than that obtained using other techniques based on the cell complete destruction.

AI evidence extraction

Main findings

Pulsed electric field (PEF) treatment enhanced extraction of C-phycocyanin from fresh Arthrospira platensis biomass, with electroporation depending on electric field strength and treatment duration. The minimum reported field intensity to detect C-phycocyanin in the extraction medium was 15 kV/cm after 150 μs (50 pulses of 3 μs). Increasing treatment temperature (10–40°C) reduced the field strength and treatment time needed for a given extraction yield and lowered specific energy input (e.g., for 100 mg/g d extraction, 25 to 18 kV/cm and 106.7 to 67.5 kJ/kg when temperature increased from 10°C to 40°C). Purity of extract from electroporated cells was higher than extracts obtained using techniques based on complete cell destruction.

Outcomes measured

• C-phycocyanin extraction yield (PEY) into aqueous media
• Detection of C-phycocyanin in extraction medium
• Purity of C-phycocyanin extract
• Electroporation dependence on field strength and treatment duration
• Specific energy input (kJ/kg)

Limitations

• Sample size not reported in abstract
• No details provided on experimental replication or statistical uncertainty beyond mention of response surface methodology
• Study is on algal biomass processing, not human/animal health outcomes

{
    "study_type": "other",
    "exposure": {
        "band": null,
        "source": "pulsed electric field (PEF) food/biomass processing",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "60–150 μs (e.g., 50 pulses of 3 μs; minimum reported 150 μs)"
    },
    "population": "Fresh biomass of Arthrospira (Artrhospira) platensis",
    "sample_size": null,
    "outcomes": [
        "C-phycocyanin extraction yield (PEY) into aqueous media",
        "Detection of C-phycocyanin in extraction medium",
        "Purity of C-phycocyanin extract",
        "Electroporation dependence on field strength and treatment duration",
        "Specific energy input (kJ/kg)"
    ],
    "main_findings": "Pulsed electric field (PEF) treatment enhanced extraction of C-phycocyanin from fresh Arthrospira platensis biomass, with electroporation depending on electric field strength and treatment duration. The minimum reported field intensity to detect C-phycocyanin in the extraction medium was 15 kV/cm after 150 μs (50 pulses of 3 μs). Increasing treatment temperature (10–40°C) reduced the field strength and treatment time needed for a given extraction yield and lowered specific energy input (e.g., for 100 mg/g d extraction, 25 to 18 kV/cm and 106.7 to 67.5 kJ/kg when temperature increased from 10°C to 40°C). Purity of extract from electroporated cells was higher than extracts obtained using techniques based on complete cell destruction.",
    "effect_direction": "benefit",
    "limitations": [
        "Sample size not reported in abstract",
        "No details provided on experimental replication or statistical uncertainty beyond mention of response surface methodology",
        "Study is on algal biomass processing, not human/animal health outcomes"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "pulsed electric field",
        "PEF",
        "electroporation",
        "Arthrospira platensis",
        "C-phycocyanin",
        "extraction yield",
        "temperature",
        "specific energy"
    ],
    "suggested_hubs": []
}

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