Exposure of human lymphocytes to sweeping-frequency extremely low frequency magnetic field

Abstract

Category: Radiobiology Tags: ELF magnetic fields, human lymphocytes, DNA damage, apoptosis, electromagnetic exposure, ion-cyclotron frequencies, health effects DOI: 10.1080/09553002.2025.2542322 URL: pubmed.ncbi.nlm.nih.gov Overview This study examines how exposure to sweeping-frequency extremely low frequency magnetic fields (ELF MF) impacts human lymphocytes. Study Design & Methods - Human umbilical cord blood lymphocytes (UCBL) were isolated and exposed for 48 hours to a sinusoidal ELF MF sweeping from 3–26 Hz. - Six different amplitudes were tested, ranging from 6 μT to 24 μT, using an oblong coil. - DNA double-strand breaks (DSB) were evaluated by assessing γH2AX, 53BP1, and co-localized γH2AX/53BP1 DNA repair foci. - Cell viability and apoptosis stages were measured, identifying viable cells, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells. Findings - No statistically significant effects on DNA repair foci or apoptosis induction were observed after 48 hours of ELF MF exposure. - A notable finding: at 8 μT amplitude, there was more than a two-fold decrease (p = .064) in γH2AX foci compared to controls, suggesting reduced DNA double-strand breaks. - Additionally, for the same field intensity, data pointed to a decrease in viable cell count. Conclusion These findings suggest potential preventive or blocking effects of sweeping-frequency ELF MF on DNA double-strand break (DSB) formation in lymphocytes. This indicates a possibility of using such magnetic fields as a protective measure against genotoxic agents and highlights the importance of thoroughly testing frequency and intensity variations for optimal biological effects. Note: Any exposure to electromagnetic fields can influence biological systems, and this study signals the relevance of further research to fully understand both potential health risks and protective effects of ELF MF exposures.

AI evidence extraction

Main findings

After 48 hours of exposure to a sinusoidal ELF magnetic field sweeping 3–26 Hz at 6–24 µT, no statistically significant effects were observed on DNA repair foci or apoptosis induction. At 8 µT, γH2AX foci were reported as more than two-fold decreased versus controls (p=.064), and data at this intensity also pointed to a decrease in viable cell count.

Outcomes measured

• DNA double-strand breaks (γH2AX, 53BP1, co-localized γH2AX/53BP1 foci)
• Cell viability
• Apoptosis stages (viable, early apoptotic, late apoptotic/necrotic)

Limitations

• Sample size not reported in the provided abstract/metadata
• Most reported outcomes described as not statistically significant; the highlighted γH2AX change at 8 µT had p=.064 (not conventionally significant)
• In vitro study using isolated lymphocytes; generalizability to in vivo human health effects is not established in the abstract

Suggested hubs

• elf-emf (0.9)
  Study investigates extremely low frequency (3–26 Hz) magnetic field exposure and biological endpoints.

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        "sar_wkg": null,
        "duration": "48 hours; sinusoidal ELF MF sweeping 3–26 Hz; amplitudes 6–24 µT (six levels)"
    },
    "population": "Human umbilical cord blood lymphocytes (UCBL)",
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    "main_findings": "After 48 hours of exposure to a sinusoidal ELF magnetic field sweeping 3–26 Hz at 6–24 µT, no statistically significant effects were observed on DNA repair foci or apoptosis induction. At 8 µT, γH2AX foci were reported as more than two-fold decreased versus controls (p=.064), and data at this intensity also pointed to a decrease in viable cell count.",
    "effect_direction": "mixed",
    "limitations": [
        "Sample size not reported in the provided abstract/metadata",
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        "In vitro study using isolated lymphocytes; generalizability to in vivo human health effects is not established in the abstract"
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        "DNA double-strand breaks",
        "γH2AX",
        "53BP1",
        "apoptosis",
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