Ouabain inhibition of kidney ATPase is altered by 9.14 GHz radiation.

Abstract

At each of several stabilized temperatures between 7.0 and 43.8 degrees C, increases in dog-kidney, Na(+)-, K(+)-ATPase catalytic activity were usually observed in association with exposure for 5 min to 9.14 GHz CW microwave radiation in a thin tubular reactor. However, at 24.9 degrees C, a 23% decrease occurred. Comparisons of activity of ouabain-inhibited reactions revealed that the efficacy of the cardiac glycoside as an inhibitor of ATPase activity was severely diminished by the microwave field. The ouabain-site control mechanism may be a specific microwave target at this exposure frequency. Experimental results can be interpreted in terms of molecular structural changes or direct energy input. The estimated SAR of energy that was incident on preparations is 20 W/kg.

AI evidence extraction

Main findings

Across several stabilized temperatures (7.0–43.8 °C), exposure for 5 min to 9.14 GHz continuous-wave microwave radiation was usually associated with increased dog-kidney Na(+), K(+)-ATPase catalytic activity, but at 24.9 °C a 23% decrease was observed. In ouabain-inhibited reactions, the efficacy of ouabain as an inhibitor was reported to be severely diminished by the microwave field.

Outcomes measured

• Na(+), K(+)-ATPase catalytic activity
• Ouabain inhibition efficacy of ATPase activity

Limitations

• Sample size not reported in abstract.
• In vitro enzyme/preparation study; generalizability to in vivo outcomes not addressed in abstract.
• Temperature-dependent effects noted; mechanism not directly established in abstract.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 9140,
        "sar_wkg": 20,
        "duration": "5 min"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Na(+), K(+)-ATPase catalytic activity",
        "Ouabain inhibition efficacy of ATPase activity"
    ],
    "main_findings": "Across several stabilized temperatures (7.0–43.8 °C), exposure for 5 min to 9.14 GHz continuous-wave microwave radiation was usually associated with increased dog-kidney Na(+), K(+)-ATPase catalytic activity, but at 24.9 °C a 23% decrease was observed. In ouabain-inhibited reactions, the efficacy of ouabain as an inhibitor was reported to be severely diminished by the microwave field.",
    "effect_direction": "mixed",
    "limitations": [
        "Sample size not reported in abstract.",
        "In vitro enzyme/preparation study; generalizability to in vivo outcomes not addressed in abstract.",
        "Temperature-dependent effects noted; mechanism not directly established in abstract."
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "9.14 GHz",
        "microwave radiation",
        "continuous wave",
        "SAR 20 W/kg",
        "dog kidney",
        "Na(+), K(+)-ATPase",
        "ouabain",
        "enzyme activity",
        "temperature dependence"
    ],
    "suggested_hubs": []
}

Comments

Log in to comment.

No comments yet.