In vitro exposure to electromagnetic fields: changes in tumour cell properties.

Abstract

Two human colon cancer cell lines, Colo 205 and Colo 320 DM, have been studied for their responses to 60 Hz-generated electromagnetic fields (EMF) using soft agar cloning and monoclonal antibody binding assays to assess exposure-induced changes. Cellular responses have been studied after 24 h continuous exposure of cells concurrently to four experimental conditions; i.e. no EMF (E-M-), magnetic field only (M+, 1.0 G rms), electric field only (E+, 300 mA/m2 rms), and combined electric plus magnetic fields at these intensities (E+M+). Under these conditions, both cell lines demonstrated significantly increased colony formation in soft agar and increased expression of tumor associated antigens after exposure to E+M+ and to M+ as compared to unexposed controls.

AI evidence extraction

Main findings

After 24 h continuous exposure, both colon cancer cell lines showed significantly increased colony formation in soft agar and increased expression of tumor-associated antigens following combined electric+magnetic field exposure (E+M+) and magnetic-field-only exposure (M+) compared with unexposed controls.

Outcomes measured

• Soft agar colony formation (cloning)
• Monoclonal antibody binding / expression of tumor-associated antigens

Limitations

• Sample size not reported in abstract
• In vitro cancer cell lines; findings may not generalize to in vivo or human health outcomes
• Only one frequency (60 Hz) and specific field intensities tested
• Short exposure duration (24 h)

Suggested hubs

• elf-emf (0.9)
  Study examines 60 Hz (ELF) electric and magnetic field exposures.
• in-vitro-studies (0.85)
  In vitro experiments on human tumor cell lines assessing biological changes.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "laboratory-generated electromagnetic fields",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "24 h continuous exposure"
    },
    "population": "Two human colon cancer cell lines (Colo 205 and Colo 320 DM)",
    "sample_size": null,
    "outcomes": [
        "Soft agar colony formation (cloning)",
        "Monoclonal antibody binding / expression of tumor-associated antigens"
    ],
    "main_findings": "After 24 h continuous exposure, both colon cancer cell lines showed significantly increased colony formation in soft agar and increased expression of tumor-associated antigens following combined electric+magnetic field exposure (E+M+) and magnetic-field-only exposure (M+) compared with unexposed controls.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "In vitro cancer cell lines; findings may not generalize to in vivo or human health outcomes",
        "Only one frequency (60 Hz) and specific field intensities tested",
        "Short exposure duration (24 h)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF",
        "60 Hz",
        "electromagnetic fields",
        "magnetic field",
        "electric field",
        "colon cancer cell lines",
        "Colo 205",
        "Colo 320 DM",
        "soft agar cloning",
        "tumor-associated antigens",
        "monoclonal antibody binding"
    ],
    "suggested_hubs": [
        {
            "slug": "elf-emf",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study examines 60 Hz (ELF) electric and magnetic field exposures."
        },
        {
            "slug": "in-vitro-studies",
            "weight": 0.84999999999999997779553950749686919152736663818359375,
            "reason": "In vitro experiments on human tumor cell lines assessing biological changes."
        }
    ]
}

Comments

Log in to comment.

No comments yet.