Fifty Hertz electromagnetic field exposure stimulates secretion of beta-amyloid peptide in cultured human neuroglioma.

Abstract

Recent epidemiological studies raise the possibility that individuals with occupational exposure to low frequency (50-60 Hz) electromagnetic fields (LF-EMF), are at increased risk of Alzheimer's disease (AD). However, the mechanisms through which LF-EMF may affect AD pathology are unknown. We here tested the hypothesis that the exposure to LF-EMF may affect amyloidogenic processes. We examined the effect of exposure to 3.1 mT 50 Hz LF-EMF on Abeta secretion in H4 neuroglioma cells stably overexpressing human mutant amyloid precursor protein. We found that overnight exposure to LF-EMF induces a significant increase of amyloid-beta peptide (Abeta) secretion, including the isoform Abeta 1-42, without affecting cell survival. These findings show for the first time that exposure to LF-EMF stimulates Abeta secretion in vitro, thus alluding to a potential link between LF-EMF exposure and APP processing in the brain.

AI evidence extraction

Main findings

Overnight exposure to 3.1 mT 50 Hz low-frequency electromagnetic fields significantly increased amyloid-beta (Abeta) secretion, including Abeta 1-42, in H4 neuroglioma cells overexpressing mutant amyloid precursor protein. Cell survival was not affected.

Outcomes measured

• Amyloid-beta (Abeta) secretion
• Abeta 1-42 secretion
• Cell survival

Limitations

• In vitro cell culture model; findings may not generalize to humans or in vivo brain physiology
• Mechanistic details beyond increased Abeta secretion are not provided in the abstract
• Sample size and replication details are not reported in the abstract

Suggested hubs

• occupational-exposure (0.35)
  Abstract frames the motivation around occupational exposure to low-frequency (50–60 Hz) EMF and Alzheimer's disease risk.

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    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": 0.05000000000000000277555756156289135105907917022705078125,
        "sar_wkg": null,
        "duration": "overnight"
    },
    "population": "H4 human neuroglioma cells stably overexpressing human mutant amyloid precursor protein",
    "sample_size": null,
    "outcomes": [
        "Amyloid-beta (Abeta) secretion",
        "Abeta 1-42 secretion",
        "Cell survival"
    ],
    "main_findings": "Overnight exposure to 3.1 mT 50 Hz low-frequency electromagnetic fields significantly increased amyloid-beta (Abeta) secretion, including Abeta 1-42, in H4 neuroglioma cells overexpressing mutant amyloid precursor protein. Cell survival was not affected.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell culture model; findings may not generalize to humans or in vivo brain physiology",
        "Mechanistic details beyond increased Abeta secretion are not provided in the abstract",
        "Sample size and replication details are not reported in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "50 Hz",
        "low frequency electromagnetic fields",
        "ELF-EMF",
        "3.1 mT",
        "H4 neuroglioma",
        "amyloid precursor protein",
        "APP processing",
        "amyloid-beta",
        "Abeta 1-42",
        "Alzheimer's disease"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.34999999999999997779553950749686919152736663818359375,
            "reason": "Abstract frames the motivation around occupational exposure to low-frequency (50–60 Hz) EMF and Alzheimer's disease risk."
        }
    ]
}

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