Variable E-cadherin expression in a MNU-induced colon tumor model in rats which exposed with 50 Hz frequency sinusoidal magnetic field.

Abstract

Inactivation of the E-cadherin system by multiple mechanisms, including both genetic and epigenetic events, plays a significant role in multistage carcinogenesis. We have investigated the effects of sinusoidal electromagnetic fields (SMF) on E-cadherin expression in an MNU (N-methyl-N-nitrosurea)-induced colon tumor model. Male wistar albino rats were used for the study. The rats were classified into four groups: I (MNU), II (SMF+MNU), III (SMF) and IV (control). After administered at MNU in 1st and 2nd groups, 2nd and 3rd groups were exposed to a sinusoidal magnetic field (SMF, 50 Hz, 5 mT) for 6 hours/day for 8 months. The expression of E-cadherin were examined in four groups of rat colon tissues by immunohistochemistry on paraffin sections. For immunohistochemical analysis, the labeled streptavidin biotin method was performed using a Vectastain Universal Quick Kit with microwave accentuation. Fisher's exact test was used for statistical analysis between proportions. Immunohistochemical studies of E-cadherin expression in this model demonstrated significant differences for cytoplasmic expression pattern. These results suggest that the electromagnetic fields result in significant alterations in cell adhesion mechanisms. This study has implications for understanding the role of fields in cell detachment in cancer metastasis. Further work is required to determine the relative effect of the magnetic fields on these phenomena.

AI evidence extraction

Main findings

Rats exposed to a 50 Hz, 5 mT sinusoidal magnetic field showed significant differences in the cytoplasmic E-cadherin expression pattern in colon tissues compared with other groups. The authors interpret this as electromagnetic-field–related alterations in cell adhesion mechanisms.

Outcomes measured

• E-cadherin expression (immunohistochemistry; cytoplasmic expression pattern)

Limitations

• Sample size not reported in abstract
• Outcome limited to immunohistochemical E-cadherin expression patterns; clinical/tumor outcomes not described
• Details of between-group comparisons and direction/magnitude of changes not provided in abstract
• Animal model; generalizability to humans is uncertain

Suggested hubs

• animal-studies (0.9)
  Rat MNU-induced colon tumor model with ELF magnetic-field exposure
• elf-magnetic-fields (0.85)
  Exposure is 50 Hz sinusoidal magnetic field (5 mT)

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        "sar_wkg": null,
        "duration": "6 hours/day for 8 months"
    },
    "population": "Male Wistar albino rats (MNU-induced colon tumor model)",
    "sample_size": null,
    "outcomes": [
        "E-cadherin expression (immunohistochemistry; cytoplasmic expression pattern)"
    ],
    "main_findings": "Rats exposed to a 50 Hz, 5 mT sinusoidal magnetic field showed significant differences in the cytoplasmic E-cadherin expression pattern in colon tissues compared with other groups. The authors interpret this as electromagnetic-field–related alterations in cell adhesion mechanisms.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "Outcome limited to immunohistochemical E-cadherin expression patterns; clinical/tumor outcomes not described",
        "Details of between-group comparisons and direction/magnitude of changes not provided in abstract",
        "Animal model; generalizability to humans is uncertain"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "E-cadherin",
        "cell adhesion",
        "colon tumor",
        "MNU",
        "rat model",
        "sinusoidal magnetic field",
        "50 Hz",
        "5 mT",
        "ELF magnetic field",
        "immunohistochemistry"
    ],
    "suggested_hubs": [
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            "reason": "Rat MNU-induced colon tumor model with ELF magnetic-field exposure"
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