Study of the Effects of 50Hz Homogeneous Magnetic Field on Expression of Bax, Bcl-2 and Caspase-3 of SNU Cells In Vitro.

Abstract

We investigate the effects of magnetic field on expression of Bax, Bcl-2 and Caspase-3 of SNU (human low differentiated gastric adenocarcinoma cell line) in this article. SNU cells were randomly divided into control groups and four treatment groups, the treatment groups were respectively irradiated by 50Hz homogeneous ferromagnetic field (0.097T) 5min, 30min, 60min and 120min. SNU cells were harvested for detection of Bax, Bcl-2 and Caspase-3 antigen with flow cytometric analysis (FCM), immunocytochemical staining and Western blot. The expression of Bax and Caspase-3 in all magnetic field exposure groups were higher than that in control by FCM, immunocytochemical stain and Western blot, but the expression of Bcl-2 in all magnetic field exposure groups were lower than that in control by FCM, immunocytochemical stain and Western blot. We concluded that possible mechanisms of apoptosis of SNU cells after 50Hz homogeneous ferromagnetic field (0.097T) treatment may be Up-regulating the expression of Bax and Caspase-3, dowm-regulating the expression of Bcl-2.

AI evidence extraction

Main findings

Compared with control, all 50 Hz magnetic field exposure groups (0.097 T; 5–120 min) showed higher Bax and Caspase-3 expression and lower Bcl-2 expression by flow cytometry, immunocytochemical staining, and Western blot.

Outcomes measured

• Bax expression
• Bcl-2 expression
• Caspase-3 expression

Limitations

• In vitro cell-line study; findings may not generalize to humans or in vivo conditions
• Sample size not reported in abstract
• Exposure metric reported as magnetic flux density (T) without additional dosimetry details

Suggested hubs

• elf-emf (0.9)
  50 Hz magnetic field exposure (ELF) in vitro.

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        "source": null,
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        "duration": "5, 30, 60, or 120 min"
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    "population": "SNU (human low differentiated gastric adenocarcinoma) cell line",
    "sample_size": null,
    "outcomes": [
        "Bax expression",
        "Bcl-2 expression",
        "Caspase-3 expression"
    ],
    "main_findings": "Compared with control, all 50 Hz magnetic field exposure groups (0.097 T; 5–120 min) showed higher Bax and Caspase-3 expression and lower Bcl-2 expression by flow cytometry, immunocytochemical staining, and Western blot.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell-line study; findings may not generalize to humans or in vivo conditions",
        "Sample size not reported in abstract",
        "Exposure metric reported as magnetic flux density (T) without additional dosimetry details"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
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        "0.097 T",
        "SNU cells",
        "gastric adenocarcinoma cell line",
        "apoptosis",
        "Bax",
        "Bcl-2",
        "Caspase-3",
        "flow cytometry",
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        "Western blot"
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