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Exposure to radiofrequency electromagnetic fields and IARC carcinogen assessment: Risk of Bias preliminary literature assessment for 10 key characteristics of human carcinogens

Research RF Safe Research Library Jan 1, 2025

This review examined experimental literature on whether RF-EMF exposures within ICNIRP (2020) limits affect IARC key characteristics of human carcinogens. It identified 159 articles and found that 38% of in vitro/in vivo measurements reported statistically significant effects, but higher study quality was associated with fewer reported effects and there was no consistent exposure-response pattern. The authors state that study diversity and generally poor quality prevent high-confidence conclusions for most key characteristics, while recommending replication of the few higher-quality positive findings under stringent standards.

The effects of short-term and long-term 2100 MHz radiofrequency radiation on adult rat auditory brainstem response

Research RF Safe Research Library Jan 1, 2025

This animal study examined 2100 MHz radiofrequency radiation exposure effects on auditory brainstem responses and brain oxidative/ultrastructural markers in adult rats. The 1-week exposure group showed prolonged ABR latencies and biochemical/structural changes consistent with oxidative stress and cellular injury. The authors report no harmful effects in the 10-week exposure condition with rest days under the studied protocol.

Electromagnetic fields from mobile phones: A risk for maintaining energy homeostasis?

Research RF Safe Research Library Jan 1, 2025

This narrative review discusses low-intensity RF-EMF exposure, primarily from mobile phones, with a focus on thermoregulation and energy homeostasis. It reports that many rodent studies at 900 MHz describe cold-like thermoregulatory and behavioral responses and molecular findings suggestive of WAT browning, while BAT transcriptional changes typical of cold exposure were not observed. The authors indicate short-term adaptations may not disrupt homeostasis, but emphasize uncertainty about long-term consequences and call for further research, including at 5G-relevant frequencies.

Characterization of the Core Temperature Response of Free-Moving Rats to 1.95 GHz Electromagnetic Fields

Research RF Safe Research Library Jan 1, 2025

This animal study measured core body temperature in free-moving male and female Sprague Dawley rats during and after 3-hour exposure to 1.95 GHz RF-EMF at multiple whole-body average SAR levels. A measurable thermal response was reported at 4 W/kg, while lower SAR conditions showed smaller or no significant temperature increases. The authors note that temperature dropped quickly after exposure ended, implying post-exposure measurements may underestimate peak heating.

Active matter as the underpinning agency for extraordinary sensitivity of biological membranes to electric fields

Research RF Safe Research Library Jan 1, 2025

This biophysics paper presents a nonequilibrium (active matter) statistical mechanics model for electromechanical biological membranes. It argues that energy-driven activity in membranes could enable detection of electric fields far below equilibrium thermal-noise limits, and reports that the model can reproduce experimental observations by tuning activity. The abstract frames this as a potential mechanistic link between weak electromagnetic fields and biological responses, while also noting future modeling directions and possible implications for exposure safety discussions.

Single exposure to near-threshold 5G millimeter wave modifies restraint stress responses in rats

Research RF Safe Research Library Jan 1, 2025

In a rat experiment (n=59), a single 40-minute whole-body 28 GHz exposure at near-threshold WBA-SAR levels was evaluated under normal and heat conditions with restraint. After accounting for sham-related restraint stress, exposure was associated with increased serum-free corticosterone 1–3 days later, especially when rectal temperature rose by >1°C. Urinary catecholamines suggested an immediate inhibitory effect on stress response (notably noradrenaline), with heat amplifying effects and linking noradrenaline to tail surface temperature.

Electromagnetic wireless remote control of mammalian transgene expression

Research RF Safe Research Library Jan 1, 2025

This animal proof-of-concept study describes an engineered nanoparticle–cell interface (EMPOWER) enabling wireless regulation of transgene expression using a 1-kHz magnetic field. Chitosan-coated multiferroic nanoparticles reportedly generate intracellular ROS that activates KEAP1/NRF2 biosensors connected to ROS-responsive promoters. In a mouse model of type 1 diabetes, implanted engineered cells expressing an EMPOWER-controlled insulin system reportedly normalized blood glucose in response to a weak magnetic field.

The Frequency of a Magnetic Field Determines the Behavior of Tumor and Non-Tumor Nerve Cell Models

Research RF Safe Research Library Jan 1, 2025

This in vitro study exposed glioblastoma (CT2A), neuroblastoma (N2A), and non-tumor astrocyte (C8D1A) cell models to a 100 μT magnetic field across 20–100 Hz for 24–72 hours. The abstract reports decreased viability and proliferation in the tumor cell models within a frequency window centered at 50 Hz, while astrocyte viability increased at 20 and 40 Hz. The authors conclude that frequency is a key determinant of cell-type-specific responses consistent with a “biological window” model.

Extremely low frequency magnetic field distracts zebrafish from a visual cognitive task

Research RF Safe Research Library Jan 1, 2025

This animal study trained adult zebrafish to perform a conditioned avoidance response to a visual cue. The visual cue was presented alone or together with an extremely low frequency sinusoidally changing magnetic field (0.3 Hz) at 0.015 mT or 0.06 mT. The abstract reports that the 0.06 mT magnetic field condition impaired learning performance and response behavior, suggesting a cross-modal distraction effect.

Sensation of electric fields in the Drosophila melanogaster larva

Research RF Safe Research Library Jan 1, 2025

This animal study reports that Drosophila melanogaster larvae can sense electric fields and exhibit robust electrotaxis toward the cathode in controlled environments. The authors identify head-tip sensory neurons required for this behavior and report calcium-imaging evidence that Gr66a-positive neurons encode field strength and orientation. The work supports electrosensation as a functional sensory modality in Drosophila larvae and demonstrates measurable neural and behavioral responses to electric fields under the studied conditions.

Electric and Magnetic Field Technologies in Agriculture: Plant Responses, Experimental Limitations, and Future Directions

Research RF Safe Research Library Jan 1, 2024

This narrative review discusses how electric and magnetic field technologies are applied in agriculture and how plants may respond. It proposes a model to explain potential mechanistic convergence between electric and magnetic field effects. The review highlights several suggested mechanisms, including membrane permeability changes, reactive oxygen species/antioxidant responses, altered ion transport, and DNA/gene expression changes.

Mitigating Heat-Induced Sperm Damage and Testicular Tissue Abnormalities: The Protective Role of Radiofrequency Radiation from Wi-Fi Routers in Rodent Models

Research RF Safe Research Library Jan 1, 2024

This rodent study examined whether 2.45 GHz Wi‑Fi router RF-EMF exposure could mitigate heat-stress-related reproductive damage in male rats. The combined RF-EMF + heat group reportedly had improved testicular structure measures and sperm quality versus heat-only, while RF-EMF alone was also reported to alter testis and sperm parameters. The authors interpret the findings as potentially consistent with an adaptive response and call for more research on mechanisms and safety.

The effects of radiofrequency exposure on male fertility: A systematic review of human observational studies with dose-response meta-analysis (SR 3)

Research RF Safe Research Library Jan 1, 2024

This systematic review and dose-response meta-analysis synthesizes human observational studies on radiofrequency EMF exposure and male fertility outcomes. It reports evidence of an association between RF exposure and poorer sperm parameters, including reduced quality, motility, and viability. The authors frame the findings as consistent with potential reproductive health risks and call for continued risk assessment and guideline development.

Effect of Exposure to 900 MHz GSM Mobile Phone Radiofrequency Radiation on Estrogen Receptor Methylation Status in Colon Cells of Male Sprague Dawley Rats

Research RF Safe Research Library Mar 1, 2017

This animal study exposed male Sprague-Dawley rats to 900 MHz GSM mobile phone RF radiation for 4 hours and assessed ERα promoter methylation in colon tissue. The authors report altered ERα methylation patterns versus controls after RF exposure. They also report no radioadaptive response in ERα methylation following a subsequent 3 Gy Co-60 gamma challenge.

Microwave frequency electromagnetic fields (EMFs) produce widespread neuropsychiatric effects including depression

Research RF Safe Research Library Jan 1, 2016

This 2016 narrative review proposes that non-thermal microwave/lower-frequency EMFs act primarily through activation of voltage-gated calcium channels (VGCCs), with calcium channel blockers reported to block EMF effects. It summarizes animal, occupational, and epidemiological literature and reports that exposures from base stations, heavy mobile phone use, and wireless smart meters are associated with neuropsychiatric symptoms, sometimes with doseresponse patterns. The author concludes that multiple lines of evidence collectively support that non-thermal microwave EMF exposures can produce diverse neuropsychiatric effects including depression.

Biological effects of extremely low frequency electric and magnetic fields: a review

Research RF Safe Research Library Jan 1, 1977

This review summarizes reported biological effects of extremely low frequency (ELF) electric and magnetic fields, describing them as significant and often acting as stressors. Reported outcomes include metabolic, hormonal, and body weight changes in rodents, lethality at high exposure levels in mice and insects, and increased mitotic index in mouse tissues/cells under specified exposure conditions. The review suggests many effects may be mediated through neuroendocrine, nervous system, or behavioral responses to field exposure.

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