Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note

PAPER manual 2022 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.1016/j.bbrep.2022.101232 Evidence Lab

Abstract

Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note Ehsan Khodamoradi, Shima Afrashi, Karim Khoshgard, Farshid Fathi, Soodeh Shahasavari, Rasool Azmoonfar, Masoud Najafi. Simultaneous effect of gamma and Wi-Fi radiation on gamma-H2Ax expression in peripheral blood of rat: A radio-protection note. Biochemistry and Biophysics Reports. Volume 30, 2022. doi: 10.1016/j.bbrep.2022.101232. Abstract Introduction Nuclear medicine patients are isolated in a room after the injection of a radiopharmaceutical. They may be active Wi-Fi option of its smartphone mobile or other environmental radiofrequency waves. The hypothesis of this study was the evaluation of increased biological effects of the simultaneous exposure to gamma-ray and the Wi-Fi waves by measuring the level of the increased double strand-breaks DNA in peripheral blood lymphocyte in the rat. Materials and methods Fifty male Wistar rats were exposed for 2, 24, and 72 h only by Wi-Fi, 99m Tc, and simultaneously by Wi-Fi and 99m Tc. The power density levels of Wi-Fi emitter at 15 cm was 4.2nW/ cm2. An activity of 100 μCi of 99m Tc was injected intraperitoneally. Blood samples were taken by cardiac puncture following general anesthesia. Mononuclear cells are extraction by Ficoll-Hypaque density gradient centrifugation. The number of gamma-H2AX foci per nucleus was counted by flow cytometry. The statistical differences between experimental groups at 2, 24, and 72 h were determined with a repeated measure's analysis of variance. The significant difference between groups at the same time was analyzed with the Kruskal- Wallis Test. Results The manner of gamma-H2AX expression was not the same for three groups in time. The number of gamma-H2AX foci between the three groups was a significant difference after 72 h. Conclusion Simultaneous Wi-Fi and gamma-ray exposures can increase the number of double-strand break DNA in peripheral blood lymphocytes to exposure of gamma-ray to 72 h after technetium injection in the rat. Open access paper: sciencedirect.com

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Male Wistar rats

Sample size

Exposure

RF wi-fi · 2, 24, and 72 h

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

Gamma-H2AX expression over time differed across the three exposure groups (Wi-Fi only, 99mTc only, and simultaneous Wi-Fi + 99mTc). The number of gamma-H2AX foci differed significantly between groups after 72 h, and the authors conclude that simultaneous Wi-Fi and gamma-ray exposures can increase double-strand break markers compared with gamma-ray exposure alone at 72 h after technetium injection in rats.

Outcomes measured

gamma-H2AX expression (gamma-H2AX foci per nucleus)
DNA double-strand breaks in peripheral blood lymphocytes

Limitations

Wi-Fi frequency not reported
SAR not reported
Details of gamma exposure/dose rate not reported beyond 99mTc activity and injection route
Outcome reporting in the abstract is limited (no group-wise values or effect sizes provided)

Suggested hubs

animal-studies (0.9)
Study conducted in Wistar rats assessing biomarker response to RF (Wi-Fi) and gamma exposure.

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "RF",
        "source": "wi-fi",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "2, 24, and 72 h"
    },
    "population": "Male Wistar rats",
    "sample_size": 50,
    "outcomes": [
        "gamma-H2AX expression (gamma-H2AX foci per nucleus)",
        "DNA double-strand breaks in peripheral blood lymphocytes"
    ],
    "main_findings": "Gamma-H2AX expression over time differed across the three exposure groups (Wi-Fi only, 99mTc only, and simultaneous Wi-Fi + 99mTc). The number of gamma-H2AX foci differed significantly between groups after 72 h, and the authors conclude that simultaneous Wi-Fi and gamma-ray exposures can increase double-strand break markers compared with gamma-ray exposure alone at 72 h after technetium injection in rats.",
    "effect_direction": "harm",
    "limitations": [
        "Wi-Fi frequency not reported",
        "SAR not reported",
        "Details of gamma exposure/dose rate not reported beyond 99mTc activity and injection route",
        "Outcome reporting in the abstract is limited (no group-wise values or effect sizes provided)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "Wi-Fi",
        "radiofrequency",
        "gamma radiation",
        "technetium-99m",
        "99mTc",
        "gamma-H2AX",
        "DNA double-strand breaks",
        "peripheral blood lymphocytes",
        "rat"
    ],
    "suggested_hubs": [
        {
            "slug": "animal-studies",
            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Study conducted in Wistar rats assessing biomarker response to RF (Wi-Fi) and gamma exposure."
        }
    ]
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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