[The cardiac injury effect of microwave radiation on rabbit and its mechanism].

Abstract

OBJECTIVE: To investigate the cardiac injury effect of different intensities microwave radiation on rabbits and its possible mechanism. METHODS: Rabbits were radiated by intensity of 50, 100, 150 and 200 mW/cm2 2450 MHz microwave for 20 min. 6 h after microwave radiation, the heart tissue was taken. ATP and mitochondria complex IV and V were measured in myocardial cells. The changes of myocardial tissue were observed by light microscopic. The expression of Caspase-3 and HSP 70 were detected by western blotting. RESULTS: The activity of ATP and mitochondria complex IV and V decreased significantly compared with normal control in cardiac tissue. 100, 150 and 200 mW/cm2 microwave radiation group vs. control group (P <0. 05). The HE staining result showed that myocardial cell appears edema, muscle fiber malalignment, cells appeared obvious injury. Results of western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in different dosage radiation group (P <0. 05). CONCLUSION: Microwave radiation has injury effect on rabbit heart. The possible mechanism may be related with inducing cell apoptosis by changing of stress level in myocardial cell.

AI evidence extraction

Main findings

Rabbits exposed to 2450 MHz microwave radiation (50–200 mW/cm2 for 20 min) showed decreased ATP and mitochondrial complex IV and V activity in cardiac tissue, with significant decreases reported for 100, 150, and 200 mW/cm2 vs control (P<0.05). Histology indicated myocardial edema, muscle fiber malalignment, and apparent cellular injury, and western blotting showed increased Caspase-3 and HSP70 expression in exposed groups (P<0.05).

Outcomes measured

• Cardiac tissue injury (histology/HE staining)
• Myocardial ATP activity
• Mitochondrial complex IV activity
• Mitochondrial complex V activity
• Caspase-3 protein expression
• HSP70 protein expression

Limitations

• Sample size not reported in abstract.
• Timing limited to a single post-exposure time point (6 h).
• Exposure metric reported as power density (mW/cm2); SAR not provided.
• Details on randomization/blinding and control conditions not described in abstract.

Suggested hubs

• rf-health-effects (0.86)
  Animal experiment assessing cardiac effects of 2450 MHz microwave (RF) exposure.

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2450,
        "sar_wkg": null,
        "duration": "20 min"
    },
    "population": "Rabbits",
    "sample_size": null,
    "outcomes": [
        "Cardiac tissue injury (histology/HE staining)",
        "Myocardial ATP activity",
        "Mitochondrial complex IV activity",
        "Mitochondrial complex V activity",
        "Caspase-3 protein expression",
        "HSP70 protein expression"
    ],
    "main_findings": "Rabbits exposed to 2450 MHz microwave radiation (50–200 mW/cm2 for 20 min) showed decreased ATP and mitochondrial complex IV and V activity in cardiac tissue, with significant decreases reported for 100, 150, and 200 mW/cm2 vs control (P<0.05). Histology indicated myocardial edema, muscle fiber malalignment, and apparent cellular injury, and western blotting showed increased Caspase-3 and HSP70 expression in exposed groups (P<0.05).",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract.",
        "Timing limited to a single post-exposure time point (6 h).",
        "Exposure metric reported as power density (mW/cm2); SAR not provided.",
        "Details on randomization/blinding and control conditions not described in abstract."
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "2450 MHz",
        "rabbit",
        "heart",
        "myocardial injury",
        "ATP",
        "mitochondria complex IV",
        "mitochondria complex V",
        "Caspase-3",
        "HSP70",
        "apoptosis",
        "power density"
    ],
    "suggested_hubs": [
        {
            "slug": "rf-health-effects",
            "weight": 0.85999999999999998667732370449812151491641998291015625,
            "reason": "Animal experiment assessing cardiac effects of 2450 MHz microwave (RF) exposure."
        }
    ]
}

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