The relationship between colony-forming ability, chromosome aberrations and incidence of micronuclei in V79 Chinese hamster cells exposed to microwave radiation.

Abstract

Cultured V79 Chinese hamster fibroblast cells were exposed to continuous radiation, frequency 7.7 GHz, power density 0.5 mW/cm2 for 15, 30 and 60 min. The effect of microwave radiation on cell survival and on the incidence and frequency of micronuclei and structural chromosome aberrations was investigated. The decrease in the number of irradiated V79 cell colonies was related to the power density applied and to the time of exposure. In comparison with the control samples there was a significantly higher frequency of specific chromosome aberrations such as dicentric and ring chromosomes in irradiated cells. The presence of micronuclei in irradiated cells confirmed the changes that had occurred in chromosome structure. These results suggest that microwave radiation can induce damage in the structure of chromosomal DNA.

AI evidence extraction

Main findings

V79 cells exposed to continuous 7.7 GHz radiation at 0.5 mW/cm2 for 15–60 min showed a decrease in colony number related to power density and exposure time. Irradiated cells had a significantly higher frequency of specific chromosome aberrations (dicentric and ring chromosomes) versus controls, and micronuclei were present in irradiated cells.

Outcomes measured

• colony-forming ability/cell survival
• micronuclei incidence and frequency
• structural chromosome aberrations (dicentric and ring chromosomes)

Limitations

• In vitro cell model (V79 Chinese hamster fibroblasts), not human or in vivo
• Sample size not reported in abstract
• Only one frequency (7.7 GHz) and one stated power density (0.5 mW/cm2) in abstract
• SAR/temperature control not described in abstract

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 7700,
        "sar_wkg": null,
        "duration": "15, 30 and 60 min"
    },
    "population": "Cultured V79 Chinese hamster fibroblast cells",
    "sample_size": null,
    "outcomes": [
        "colony-forming ability/cell survival",
        "micronuclei incidence and frequency",
        "structural chromosome aberrations (dicentric and ring chromosomes)"
    ],
    "main_findings": "V79 cells exposed to continuous 7.7 GHz radiation at 0.5 mW/cm2 for 15–60 min showed a decrease in colony number related to power density and exposure time. Irradiated cells had a significantly higher frequency of specific chromosome aberrations (dicentric and ring chromosomes) versus controls, and micronuclei were present in irradiated cells.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro cell model (V79 Chinese hamster fibroblasts), not human or in vivo",
        "Sample size not reported in abstract",
        "Only one frequency (7.7 GHz) and one stated power density (0.5 mW/cm2) in abstract",
        "SAR/temperature control not described in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "V79 cells",
        "Chinese hamster fibroblasts",
        "microwave radiation",
        "7.7 GHz",
        "power density 0.5 mW/cm2",
        "colony-forming ability",
        "cell survival",
        "chromosome aberrations",
        "dicentric chromosomes",
        "ring chromosomes",
        "micronuclei",
        "genotoxicity"
    ],
    "suggested_hubs": []
}

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