Ascorbic acid changes in cultured rabbit lenses after microwave irradiation.

Abstract

Whole body exposure of rabbits to microwave radiation causes a decrease in ascorbic acid in the lens. In our study, rabbit lenses maintained in culture medium (37 degrees C) were exposed to either pulsed or continuous wave S-band radiation for 10-15 min at power densities between 0 and 200 mW/cm-2. Total ascorbic acid was measured in selected lenses 1-3 days after irradiation. The temperature of the culture medium was measured during irradiation. Matched control lenses were exposed to similar time-temperature environments, but without microwave irradiation. Ascorbic acid decreased significantly in lenses exposed to microwave radiation. No differences were found, however, between irradiated and control lenses subjected to identical time-temperature conditions. At a given average power density, the time-temperature variation was independent of modulation. A decrease in ascorbic acid is apparently a direct thermal effect of microwave radiation in rabbit lens culture.

AI evidence extraction

Main findings

Cultured rabbit lenses exposed to pulsed or continuous wave S-band microwave radiation (10–15 min; 0–200 mW/cm^2) showed a significant decrease in total ascorbic acid 1–3 days after irradiation. However, when irradiated lenses were compared with matched control lenses subjected to identical time–temperature conditions without microwaves, no differences in ascorbic acid were found; the decrease was interpreted as a direct thermal effect.

Outcomes measured

• Lens total ascorbic acid
• Culture medium temperature/time-temperature variation during irradiation

Limitations

• Frequency not reported beyond 'S-band'
• Sample size not reported
• In vitro lens culture model; may not generalize to whole-animal or human exposure
• Outcome assessed 1–3 days post-irradiation only

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "10–15 min"
    },
    "population": "Cultured rabbit lenses (rabbit lens culture)",
    "sample_size": null,
    "outcomes": [
        "Lens total ascorbic acid",
        "Culture medium temperature/time-temperature variation during irradiation"
    ],
    "main_findings": "Cultured rabbit lenses exposed to pulsed or continuous wave S-band microwave radiation (10–15 min; 0–200 mW/cm^2) showed a significant decrease in total ascorbic acid 1–3 days after irradiation. However, when irradiated lenses were compared with matched control lenses subjected to identical time–temperature conditions without microwaves, no differences in ascorbic acid were found; the decrease was interpreted as a direct thermal effect.",
    "effect_direction": "unclear",
    "limitations": [
        "Frequency not reported beyond 'S-band'",
        "Sample size not reported",
        "In vitro lens culture model; may not generalize to whole-animal or human exposure",
        "Outcome assessed 1–3 days post-irradiation only"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave irradiation",
        "S-band",
        "pulsed",
        "continuous wave",
        "power density",
        "rabbit lens",
        "lens culture",
        "ascorbic acid",
        "thermal effect",
        "temperature control"
    ],
    "suggested_hubs": []
}

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