Microwave radiation-induced chromosomal aberrations in corneal epithelium of Chinese hamsters.

Abstract

A microwave diathermy machine was used to irradiate the eyes of 5-month-old female Chinese hamsters. The right eye of each of seven animals was irradiated with 75 mW/cm2 radiation density for 10 minutes. After one month, slit-lamp examinations revealed lens opacities in the exposed eyes of two animals. Next, the right eye of each of 32 animals was irradiated with 100 mW/cm2 radiation density for 30, 20, 10, or 5 minutes. Epithelial cells of the cornea were collected to make chromosomal preparations. There were 0.1562, 0.0794, 0.0819, and 0.0488 chromosomal breaks per cell, respectively. No chromosomal breaks were observed in three sham-exposed animals. The percentage of abnormal cells and the number of chromosomal breaks per cell in animals that had exposures of 100 mW/cm2 radiation density for 30 minutes were higher than those in control animals. These results were statistically significant at the 5 percent level.

AI evidence extraction

Main findings

After 75 mW/cm2 for 10 minutes, lens opacities were observed in 2 of 7 exposed eyes at one month. After 100 mW/cm2 exposures, chromosomal breaks per cell were reported as 0.1562 (30 min), 0.0794 (20 min), 0.0819 (10 min), and 0.0488 (5 min), while no chromosomal breaks were observed in three sham-exposed animals. The percentage of abnormal cells and chromosomal breaks per cell were higher than controls for the 100 mW/cm2, 30-minute exposure and were statistically significant at the 5% level.

Outcomes measured

• Chromosomal aberrations (chromosomal breaks per cell; % abnormal cells) in corneal epithelium
• Lens opacities (slit-lamp examination)

Limitations

• Frequency not reported
• SAR not reported
• Small sham-exposed control group (n=3) reported for chromosomal breaks
• Only right eye exposed; details of randomization/blinding not described in abstract
• Limited follow-up information (lens opacities assessed at one month only for the 75 mW/cm2 condition)

Suggested hubs

• animal-studies (0.7)
  In vivo hamster eye irradiation with microwave diathermy and cytogenetic outcomes

{
    "study_type": "animal",
    "exposure": {
        "band": "microwave",
        "source": "microwave diathermy machine",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "75 mW/cm2 for 10 minutes (n=7); 100 mW/cm2 for 30, 20, 10, or 5 minutes (n=32)"
    },
    "population": "5-month-old female Chinese hamsters",
    "sample_size": 39,
    "outcomes": [
        "Chromosomal aberrations (chromosomal breaks per cell; % abnormal cells) in corneal epithelium",
        "Lens opacities (slit-lamp examination)"
    ],
    "main_findings": "After 75 mW/cm2 for 10 minutes, lens opacities were observed in 2 of 7 exposed eyes at one month. After 100 mW/cm2 exposures, chromosomal breaks per cell were reported as 0.1562 (30 min), 0.0794 (20 min), 0.0819 (10 min), and 0.0488 (5 min), while no chromosomal breaks were observed in three sham-exposed animals. The percentage of abnormal cells and chromosomal breaks per cell were higher than controls for the 100 mW/cm2, 30-minute exposure and were statistically significant at the 5% level.",
    "effect_direction": "harm",
    "limitations": [
        "Frequency not reported",
        "SAR not reported",
        "Small sham-exposed control group (n=3) reported for chromosomal breaks",
        "Only right eye exposed; details of randomization/blinding not described in abstract",
        "Limited follow-up information (lens opacities assessed at one month only for the 75 mW/cm2 condition)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave",
        "diathermy",
        "Chinese hamster",
        "cornea",
        "corneal epithelium",
        "chromosomal aberrations",
        "chromosomal breaks",
        "lens opacities",
        "eye exposure"
    ],
    "suggested_hubs": [
        {
            "slug": "animal-studies",
            "weight": 0.6999999999999999555910790149937383830547332763671875,
            "reason": "In vivo hamster eye irradiation with microwave diathermy and cytogenetic outcomes"
        }
    ]
}

Comments

Log in to comment.

No comments yet.