Human osteosarcoma cells in response to ELF-MF: Morphological remodeling compared to cell proliferation.

PAPER pubmed Journal of cancer research and therapeutics 2023 In vitro study Effect: harm Evidence: Low

Read original paper → DOI: 10.4103/jcrt.jcrt_1848_21 PMID: 37787300 Evidence Lab

Abstract

PURPOSE: The present study aimed to assess the effects of extremely low-frequency electromagnetic fields (ELF-MF) on structural changes of human osteosarcoma cells by analyzing the stained cytoskeleton for assessing the relationship between the fractal dimension parameter and proliferation rate of radiation-induced cells. MATERIALS AND METHODS: In this study, 2-mT magnetic fields with various waveforms, including sinusoidal, triangular, and pulsed shapes, were employed to determine the biological effects of ELF-EMF on the human osteosarcoma MG-63 cell line. All experiments were performed in two modes: continuous exposure at 3 h and fractionated irradiations at 3 consecutive days. Afterward, the proliferation assay was implemented for assessing the cell proliferation in each group. Moreover, immunofluorescence staining and confocal imaging were performed to determine the cell shape index. Furthermore, fractal dimension analysis was carried out by processing morphological images. RESULTS: The proliferation and shape index parameters of radiation-induced osteosarcomas significantly decreased compared with non-irradiated cells. In addition, fractal dimensions significantly increased following fractionated exposure at 3 consecutive days. CONCLUSIONS: Assessing the fractal dimensions can be considered as a new morphological index for the prognosis of the structural remodeling of human osteosarcoma cells in response to fractionated irradiation of ELF-MF. In addition, various waveforms induce a similar effect on morphological remodeling and cell proliferation.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

harm

Population

Human osteosarcoma MG-63 cell line

Sample size

—

Exposure

ELF · Continuous exposure at 3 h; fractionated irradiations at 3 consecutive days

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

Exposure to 2 mT ELF-MF (sinusoidal, triangular, and pulsed waveforms) was associated with significant decreases in proliferation and shape index compared with non-irradiated cells. Fractal dimensions significantly increased following fractionated exposure over 3 consecutive days. The authors report similar effects across the different waveforms on morphological remodeling and cell proliferation.

Outcomes measured

Cell proliferation (proliferation assay)
Cell morphology/shape index (immunofluorescence staining, confocal imaging)
Cytoskeleton structural changes
Fractal dimension of morphological images

Limitations

Sample size not reported in abstract
Frequency of ELF-MF not specified in abstract
In vitro cell-line study; generalizability to humans/clinical outcomes not addressed in abstract
Details of exposure conditions and controls beyond duration/waveforms not provided in abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "Continuous exposure at 3 h; fractionated irradiations at 3 consecutive days"
    },
    "population": "Human osteosarcoma MG-63 cell line",
    "sample_size": null,
    "outcomes": [
        "Cell proliferation (proliferation assay)",
        "Cell morphology/shape index (immunofluorescence staining, confocal imaging)",
        "Cytoskeleton structural changes",
        "Fractal dimension of morphological images"
    ],
    "main_findings": "Exposure to 2 mT ELF-MF (sinusoidal, triangular, and pulsed waveforms) was associated with significant decreases in proliferation and shape index compared with non-irradiated cells. Fractal dimensions significantly increased following fractionated exposure over 3 consecutive days. The authors report similar effects across the different waveforms on morphological remodeling and cell proliferation.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract",
        "Frequency of ELF-MF not specified in abstract",
        "In vitro cell-line study; generalizability to humans/clinical outcomes not addressed in abstract",
        "Details of exposure conditions and controls beyond duration/waveforms not provided in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low-frequency electromagnetic fields",
        "ELF-MF",
        "2 mT",
        "waveform",
        "sinusoidal",
        "triangular",
        "pulsed",
        "osteosarcoma",
        "MG-63",
        "cell proliferation",
        "cytoskeleton",
        "morphological remodeling",
        "shape index",
        "fractal dimension",
        "fractionated exposure"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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