[Effect of an industrial-frequency electromagnetic field on protein biosynthesic processes of embryonal fibroblasts in tissue culture].

Abstract

Using radioautography, the influence of the industrial frequency electromagnetic field (50 cps) (EMF i/f), with the tension of 50 and 150 kv/m was studied, on the synthesis of collagen and non-collagen proteins in cultured fibroblast-like human embryo cells in the presence and absense in the medium of adrenaline and of cyclic adenosine monophosphate (cAMP). The EMF depresses protein proportionally to its intesity and duration. The specific function of fibroblasts -- the synthesis of collagen -- is subject to this influence to a great extent. The preliminary administration of adrenaline or cAMP prevented the above changes. The cyclic AMP exerted a much stronger protective effect.

AI evidence extraction

Main findings

In cultured fibroblast-like human embryo cells, exposure to an industrial-frequency electromagnetic field (50 cps) at 50 or 150 kV/m depressed protein synthesis proportionally to field intensity and duration, with collagen synthesis affected to a greater extent. Preliminary administration of adrenaline or cAMP prevented these changes, with cAMP described as having a stronger protective effect.

Outcomes measured

• Collagen protein synthesis
• Non-collagen protein synthesis
• Effect modification by adrenaline
• Effect modification by cyclic adenosine monophosphate (cAMP)

Limitations

• In vitro tissue culture model; findings may not generalize to in vivo human exposure
• Sample size not reported in abstract
• Exposure duration not specified in abstract
• Details of experimental design and statistical analysis not provided in abstract

Suggested hubs

• occupational-exposure (0.35)
  Study uses industrial-frequency (50 cps) field exposure, relevant to occupational/industrial EMF contexts.

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": "other",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Cultured fibroblast-like human embryo cells (embryonal fibroblasts)",
    "sample_size": null,
    "outcomes": [
        "Collagen protein synthesis",
        "Non-collagen protein synthesis",
        "Effect modification by adrenaline",
        "Effect modification by cyclic adenosine monophosphate (cAMP)"
    ],
    "main_findings": "In cultured fibroblast-like human embryo cells, exposure to an industrial-frequency electromagnetic field (50 cps) at 50 or 150 kV/m depressed protein synthesis proportionally to field intensity and duration, with collagen synthesis affected to a greater extent. Preliminary administration of adrenaline or cAMP prevented these changes, with cAMP described as having a stronger protective effect.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro tissue culture model; findings may not generalize to in vivo human exposure",
        "Sample size not reported in abstract",
        "Exposure duration not specified in abstract",
        "Details of experimental design and statistical analysis not provided in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "industrial frequency",
        "50 cps",
        "ELF",
        "electric field",
        "kV/m",
        "fibroblasts",
        "human embryo cells",
        "collagen synthesis",
        "protein synthesis",
        "adrenaline",
        "cAMP",
        "radioautography"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.34999999999999997779553950749686919152736663818359375,
            "reason": "Study uses industrial-frequency (50 cps) field exposure, relevant to occupational/industrial EMF contexts."
        }
    ]
}

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