Stimulation of protein kinase A activity and induced terminal differentiation of human skin fibroblasts in culture by low-frequency electromagnetic fields.

PAPER pubmed Toxicology letters 1998 In vitro study Effect: mixed Evidence: Low

Read original paper → DOI: 10.1016/s0378-4274(98)00095-2 PMID: 9820690 Evidence Lab

Abstract

In the present study experiments are described, which indicate that the exposure of normal human skin fibroblasts (HSFs) to a low-frequency electromagnetic field of 20 Hz and 7-8 mT does induce terminal differentiation within 14 days of daily exposure of 12 h. As demonstrated by the analysis of the expression level of the proto-oncogene c-myc, the induction of terminal differentiation of progenitor fibroblasts to postmitotic fibrocytes does most likely not involve changes in the c-myc protein. As one possible candidate being involved in the ELF-EMF-mediated inhibition of fibroblast growth and the subsequent induction of terminal differentiation the cyclic AMP-dependent protein kinase A (PKA) could be characterised. Thus, the data presented clearly indicate that the specific EMF field of 20 Hz and 7-8 mT significantly interfere with regulatory processes of fibroblast proliferation and differentiation.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

mixed

Population

Normal human skin fibroblasts (HSFs) in culture

Sample size

—

Exposure

ELF · 2.0E-5 MHz · 14 days; daily exposure 12 h

Evidence strength

Low

Confidence: 74% · Peer-reviewed: yes

Main findings

Exposure of normal human skin fibroblasts to a 20 Hz, 7–8 mT electromagnetic field induced terminal differentiation within 14 days with 12 h/day exposure. The induction of terminal differentiation was reported to most likely not involve changes in c-myc protein, and PKA was characterized as a possible candidate involved in ELF-EMF-mediated growth inhibition and subsequent differentiation.

Outcomes measured

Terminal differentiation of human skin fibroblasts
Fibroblast proliferation/growth inhibition
Protein kinase A (PKA) activity (cyclic AMP-dependent)
c-myc proto-oncogene expression level / c-myc protein involvement

Limitations

In vitro cell culture study; findings may not generalize to in vivo human health outcomes
Sample size and replication details not provided in the abstract
Mechanistic conclusions (e.g., PKA involvement) described as a possible candidate rather than definitively established

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 2.000000000000000163606107828062619091724627651274204254150390625e-5,
        "sar_wkg": null,
        "duration": "14 days; daily exposure 12 h"
    },
    "population": "Normal human skin fibroblasts (HSFs) in culture",
    "sample_size": null,
    "outcomes": [
        "Terminal differentiation of human skin fibroblasts",
        "Fibroblast proliferation/growth inhibition",
        "Protein kinase A (PKA) activity (cyclic AMP-dependent)",
        "c-myc proto-oncogene expression level / c-myc protein involvement"
    ],
    "main_findings": "Exposure of normal human skin fibroblasts to a 20 Hz, 7–8 mT electromagnetic field induced terminal differentiation within 14 days with 12 h/day exposure. The induction of terminal differentiation was reported to most likely not involve changes in c-myc protein, and PKA was characterized as a possible candidate involved in ELF-EMF-mediated growth inhibition and subsequent differentiation.",
    "effect_direction": "mixed",
    "limitations": [
        "In vitro cell culture study; findings may not generalize to in vivo human health outcomes",
        "Sample size and replication details not provided in the abstract",
        "Mechanistic conclusions (e.g., PKA involvement) described as a possible candidate rather than definitively established"
    ],
    "evidence_strength": "low",
    "confidence": 0.7399999999999999911182158029987476766109466552734375,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "ELF-EMF",
        "20 Hz",
        "7-8 mT",
        "human skin fibroblasts",
        "terminal differentiation",
        "proliferation",
        "protein kinase A",
        "PKA",
        "c-myc"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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