Enhancement of nitric oxide generation by low frequency electromagnetic field.

PAPER pubmed Pathophysiology : the official journal of the International Society for Pathophysiology 2000 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.1016/s0928-4680(00)00040-7 PMID: 10927193 Evidence Lab

Abstract

Oxidative stress is implicated in the intracellular signal transduction pathways for nitric oxide synthase (NOS) induction. The electromagnetic field (EMF) is believed to increase the free radical lifespan [S. Roy, Y. Noda, V. Eckert, M.G. Traber, A. Mori, R. Liburdy, L. Packer, The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field, FEBS Lett. 376 (1995) 164-6; F.S. Prato, M. Kavaliers, J.J. Carson, Behavioural evidence that magnetic field effects in the land snail, Cepaea nemoralis, might not depend on magnetite or induced electric currents, Bioelectromagnetics 17 (1996) 123-30; A.L. Hulbert, J. Metcalfe, R. Hesketh, Biological response to electromagnetic fields, FASEB 12 (1998) 395-420]. We tested the effects of EMF on endotoxin induced nitric oxide (NO) generation in vivo. Male BALB/C mice were injected with lipopolysaccharide (LPS) intraperitoneously (i.p.), followed by the exposure to EMF (0.1 mT, 60 Hz). Five hours and 30 min after the LPS administration, mice were administered with a NO spin trap, ferrous N-methyl-D-glucaminedithiocarbamate (MGD-Fe). Thirty minutes later, mice were sacrificed, and their livers were removed. The results were compared to three control groups: group A (LPS (-) EMF(-)); group B (LPS(-) EMF(+)); group C (LPS(+) EMF(-)). The ESR spectra of obtained livers were examined at room temperature. Three-line spectra of NO adducts were observed in the livers of all groups. In groups A and B very weak signals were observed, but in groups C and D strong spectra were observed. The signal intensity of the NO adducts in Group D was also significantly stronger than that in Group C. EMF itself did not induce NO generation, however, it enhanced LPS induced NO generation in vivo.

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Male BALB/C mice

Sample size

—

Exposure

ELF · 6.0E-5 MHz

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

In mice, exposure to a 0.1 mT, 60 Hz electromagnetic field did not induce nitric oxide (NO) generation by itself, but significantly enhanced lipopolysaccharide (LPS)-induced NO generation in vivo as measured by ESR spectra of NO adducts in liver.

Outcomes measured

Nitric oxide (NO) generation in vivo (ESR detection of NO adducts in liver)

Limitations

Sample size not reported in abstract.
Exposure duration/timing details for EMF not fully specified in abstract.
Outcome measured in liver only; generalizability to other tissues not addressed in abstract.
Animal model with LPS challenge; relevance to typical human exposures not established in abstract.

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "ELF",
        "source": null,
        "frequency_mhz": 6.00000000000000015200514458246772164784488268196582794189453125e-5,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Male BALB/C mice",
    "sample_size": null,
    "outcomes": [
        "Nitric oxide (NO) generation in vivo (ESR detection of NO adducts in liver)"
    ],
    "main_findings": "In mice, exposure to a 0.1 mT, 60 Hz electromagnetic field did not induce nitric oxide (NO) generation by itself, but significantly enhanced lipopolysaccharide (LPS)-induced NO generation in vivo as measured by ESR spectra of NO adducts in liver.",
    "effect_direction": "harm",
    "limitations": [
        "Sample size not reported in abstract.",
        "Exposure duration/timing details for EMF not fully specified in abstract.",
        "Outcome measured in liver only; generalizability to other tissues not addressed in abstract.",
        "Animal model with LPS challenge; relevance to typical human exposures not established in abstract."
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "extremely low frequency",
        "ELF",
        "magnetic field",
        "60 Hz",
        "0.1 mT",
        "nitric oxide",
        "NO",
        "nitric oxide synthase",
        "NOS",
        "lipopolysaccharide",
        "LPS",
        "oxidative stress",
        "electron spin resonance",
        "ESR",
        "mouse",
        "liver"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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