Continuous exposure to 900MHz GSM-modulated EMF alters morphological maturation of neural cells.

PAPER pubmed Neuroscience letters 2009 In vitro study Effect: harm Evidence: Low

Read original paper → DOI: 10.1016/j.neulet.2009.03.061 PMID: 19429115 Evidence Lab

Abstract

The effects of radiofrequency electromagnetic field (RF-EMF) exposure on neuronal phenotype maturation have been studied in two different in vitro models: murine SN56 cholinergic cell line and rat primary cortical neurons. The samples were exposed at a dose of 1W/kg at 900 MHz GSM modulated. The phenotype analysis was carried out at 48 and 72 h (24 and 48 h of SN56 cell line differentiation) or at 24, 72, 120 h (2, 4 and 6 days in vitro for cortical neurons) of exposure, on live and immunolabeled neurons, and included the morphological study of neurite emission, outgrowth and branching. Moreover, cortical neurons were studied to detect alterations in the expression pattern of cytoskeleton regulating factors, e.g. beta-thymosin, and of early genes, e.g. c-Fos and c-Jun through real-time PCR on mRNA extracted after 24h exposure to EMF. We found that RF-EMF exposure reduced the number of neurites generated by both cell systems, and this alteration correlates to increased expression of beta-thymosin mRNA.

AI evidence extraction

At a glance

Study type

In vitro study

Effect direction

harm

Population

Murine SN56 cholinergic cell line and rat primary cortical neurons (in vitro)

Sample size

—

Exposure

RF · 900 MHz · 1 W/kg · 24–120 h (timepoints at 24, 48, 72, 120 h depending on model)

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

In both in vitro models, 900 MHz GSM-modulated RF-EMF exposure at 1 W/kg reduced the number of neurites generated. This morphological alteration correlated with increased beta-thymosin mRNA expression after 24 h exposure.

Outcomes measured

Neuronal phenotype maturation (morphology)
Neurite emission/generation
Neurite outgrowth
Neurite branching
mRNA expression of cytoskeleton regulating factors (beta-thymosin)
mRNA expression of early genes (c-Fos, c-Jun)

Limitations

In vitro models only (murine cell line and rat primary neurons)
Sample size not reported in the abstract
Limited exposure conditions reported (single frequency/modulation and SAR)
Gene expression results described for beta-thymosin correlation; results for c-Fos/c-Jun not reported in the abstract

View raw extracted JSON

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "RF",
        "source": null,
        "frequency_mhz": 900,
        "sar_wkg": 1,
        "duration": "24–120 h (timepoints at 24, 48, 72, 120 h depending on model)"
    },
    "population": "Murine SN56 cholinergic cell line and rat primary cortical neurons (in vitro)",
    "sample_size": null,
    "outcomes": [
        "Neuronal phenotype maturation (morphology)",
        "Neurite emission/generation",
        "Neurite outgrowth",
        "Neurite branching",
        "mRNA expression of cytoskeleton regulating factors (beta-thymosin)",
        "mRNA expression of early genes (c-Fos, c-Jun)"
    ],
    "main_findings": "In both in vitro models, 900 MHz GSM-modulated RF-EMF exposure at 1 W/kg reduced the number of neurites generated. This morphological alteration correlated with increased beta-thymosin mRNA expression after 24 h exposure.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro models only (murine cell line and rat primary neurons)",
        "Sample size not reported in the abstract",
        "Limited exposure conditions reported (single frequency/modulation and SAR)",
        "Gene expression results described for beta-thymosin correlation; results for c-Fos/c-Jun not reported in the abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "RF-EMF",
        "900 MHz",
        "GSM",
        "SAR 1 W/kg",
        "in vitro",
        "SN56",
        "primary cortical neurons",
        "neurite outgrowth",
        "neurite branching",
        "beta-thymosin",
        "c-Fos",
        "c-Jun"
    ],
    "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

Comments

No comments yet.