[Effects of high-frequency electromagnetic field on morphology of hippocampal cells in female rats].

Abstract

OBJECTIVE: To analyze the effects of high-frequency electromagnetic field (HF-EMF, 30 MHz, 0-1600 V/m) on the apoptosis and ultramicrostructure of the hippocamp and demonstrate the cytotoxicity of hippocamp. METHODS: 120 Wistar female adult rats were randomly divided into ten groups based on body weight with different levels of 30 MHz electromagnetic field (0, 25, 100, 400, 1600 V/m) for eight hours daily. Five group rats were irradiated for three days. The other five group rats were irradiated for fifty-six days. Weekly the rats were continuously exposed five days. The apoptotic rate of the hippocamp was detected with TUNEL System. Meanwhile, the ultramicrostructure was observed with the transmission electron microscope. RESULTS: (1) There was no significant difference on the apoptotic rate and pathological change of the hippocamp cell between the exposure and the control groups through short term experiment (P > 0.05). (2) The apoptotic rate of the granulocyte on the DG campus of the hippocamp in the 400 V/m group and the 1600 V/m group (0.165% +/- 0.049%, 0.189% +/- 0.049% respectively) were increased significantly (P < 0.01) through inferior chronic experiment compared with the control group (0.052% +/- 0.016%). Along with the increase of radiation dose, the ultramicrostructure of the neuron cell appeared more abnormal cells. Especially there were marked change on the neuron in the 1600 V/m group. CONCLUSIONS: There is no association between cell apoptotic rate of the hippocamp and short period exposure to HF-EMF (30 MHz, 25-1600 V/m). However inferior chronic exposures to HF-EMF might induce the cytotoxicity, especially in the high dose exposure (1600 V/m) under our experiment.

AI evidence extraction

Main findings

Short-term exposure (3 days) to 30 MHz HF-EMF at 25-1600 V/m showed no significant effect on hippocampal cell apoptosis or morphology. Chronic exposure (56 days) at higher intensities (400 and 1600 V/m) significantly increased apoptotic rates and caused abnormal neuronal ultrastructure, especially at 1600 V/m.

Outcomes measured

• apoptotic rate of hippocamp cells
• ultramicrostructure of hippocamp neurons

Limitations

• Study conducted in rats, limiting direct human applicability
• Exposure intensity measured in V/m without SAR values
• Only female rats studied
• No behavioral or functional outcomes assessed

Suggested hubs

• occupational-exposure (0.8)
  Study of high-frequency EMF exposure effects relevant to occupational settings

{
    "study_type": "randomized_trial",
    "exposure": {
        "band": "high-frequency",
        "source": null,
        "frequency_mhz": 30,
        "sar_wkg": null,
        "duration": "3 days or 56 days, 8 hours daily, 5 days per week"
    },
    "population": "120 Wistar female adult rats",
    "sample_size": 120,
    "outcomes": [
        "apoptotic rate of hippocamp cells",
        "ultramicrostructure of hippocamp neurons"
    ],
    "main_findings": "Short-term exposure (3 days) to 30 MHz HF-EMF at 25-1600 V/m showed no significant effect on hippocampal cell apoptosis or morphology. Chronic exposure (56 days) at higher intensities (400 and 1600 V/m) significantly increased apoptotic rates and caused abnormal neuronal ultrastructure, especially at 1600 V/m.",
    "effect_direction": "harm",
    "limitations": [
        "Study conducted in rats, limiting direct human applicability",
        "Exposure intensity measured in V/m without SAR values",
        "Only female rats studied",
        "No behavioral or functional outcomes assessed"
    ],
    "evidence_strength": "moderate",
    "confidence": 0.6999999999999999555910790149937383830547332763671875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "high-frequency electromagnetic field",
        "hippocampus",
        "apoptosis",
        "ultrastructure",
        "rats",
        "cytotoxicity"
    ],
    "suggested_hubs": [
        {
            "slug": "occupational-exposure",
            "weight": 0.8000000000000000444089209850062616169452667236328125,
            "reason": "Study of high-frequency EMF exposure effects relevant to occupational settings"
        }
    ]
}

Comments

Log in to comment.

No comments yet.