[Influence of magnetic field on nitric oxide in hypothalamus and its relation to hypothalamic neuroendocrine nuclei].

Abstract

By employing nitric acid reductase-spectrophotometry and NADPH-diaphorase/AVP cytochemistry technique, the effects of magnetic field on NO in hypothalamus and relations to Paraventricular Nucleus (PVN), Periventricular Nucleus (PEN), Supraoptic Nucleus (SON) and Suprachiasmatic Nucleus (SCN) were investigated. It was found that the NADPH-d positive neurons and some NADPH-d/AVP dually stained neurons existed in PVN, PEN, SON, but not in SCN, and the magnetic field induced NO (OD) increase there and the high NO (OD) level lasted for 3 hours. The results suggested that NO (OD) increase after the treatment of magnetic field in hypothalamus may result from strong expression of NOergic neurons in the PVN, PEN and SON. The coexistance of NO and AVP may play important role in the regulation of endocrine and neuroendocrine by the magnetic field.

AI evidence extraction

Main findings

Magnetic field exposure was associated with an increase in hypothalamic NO (OD) in PVN, PEN, and SON, with elevated NO (OD) lasting for 3 hours. NADPH-d positive neurons and some NADPH-d/AVP dually stained neurons were observed in PVN, PEN, and SON but not in SCN.

Outcomes measured

• Nitric oxide (NO) level (OD) in hypothalamus
• NADPH-diaphorase positive neurons distribution in PVN, PEN, SON, SCN
• NADPH-d/AVP dually stained neurons presence

Limitations

• No magnetic field parameters (e.g., intensity, frequency) reported in abstract
• Species/animal model not specified in abstract
• Sample size not reported
• Outcome is a biochemical/cytochemical marker; clinical or functional endpoints not reported

Suggested hubs

• animal-studies (0.78)
  Study investigates magnetic-field effects on hypothalamic NO and neuroendocrine nuclei using cytochemistry, consistent with an animal experimental design.

{
    "study_type": "animal",
    "exposure": {
        "band": null,
        "source": "magnetic field",
        "frequency_mhz": null,
        "sar_wkg": null,
        "duration": "high NO (OD) level lasted for 3 hours"
    },
    "population": null,
    "sample_size": null,
    "outcomes": [
        "Nitric oxide (NO) level (OD) in hypothalamus",
        "NADPH-diaphorase positive neurons distribution in PVN, PEN, SON, SCN",
        "NADPH-d/AVP dually stained neurons presence"
    ],
    "main_findings": "Magnetic field exposure was associated with an increase in hypothalamic NO (OD) in PVN, PEN, and SON, with elevated NO (OD) lasting for 3 hours. NADPH-d positive neurons and some NADPH-d/AVP dually stained neurons were observed in PVN, PEN, and SON but not in SCN.",
    "effect_direction": "harm",
    "limitations": [
        "No magnetic field parameters (e.g., intensity, frequency) reported in abstract",
        "Species/animal model not specified in abstract",
        "Sample size not reported",
        "Outcome is a biochemical/cytochemical marker; clinical or functional endpoints not reported"
    ],
    "evidence_strength": "very_low",
    "confidence": 0.61999999999999999555910790149937383830547332763671875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "magnetic field",
        "hypothalamus",
        "nitric oxide",
        "NADPH-diaphorase",
        "arginine vasopressin",
        "PVN",
        "PEN",
        "SON",
        "SCN",
        "neuroendocrine"
    ],
    "suggested_hubs": [
        {
            "slug": "animal-studies",
            "weight": 0.7800000000000000266453525910037569701671600341796875,
            "reason": "Study investigates magnetic-field effects on hypothalamic NO and neuroendocrine nuclei using cytochemistry, consistent with an animal experimental design."
        }
    ]
}

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