[Effect of low-intensity microwave radiation on proliferation of cultured epithelial cells of rabbit lens].

Abstract

OBJECTIVE: To compare the effects of different doses of microwave on the proliferative activity and cell cycle of cultured epithelial cells of rabbit lens, and to investigate the limit tolerant of microwave exposure. METHODS: Cultured epithelial cells of rabbit lens were exposed to microwave radiation with frequency of 2,450 MHz and power density of 0.10, 0.25, 0.50, 1.00, 2.00 mW/cm(2) for 8 h in vitro. HE staining was used to observe the morphological changes of lens epithelial cells, the proliferative activity and cell cycle were measured by MTT assay and PI fluorescent staining. RESULTS: 8 h after radiation, 0.50, 1.00 and 2.00 mW/cm(2) microwave could decrease the proliferation of lens epithelial cells, make the cells disordered arrangement, shrinkage, detachment, and inhibit the synthesis of cell DNA. The percentage of G(0)/G(1) phase cells were 71.95% +/- 2.12%, 75.68% +/- 3.35% and 82.40% +/- 8.68% respectively, which were higher than that in control group (61.68% +/- 5.76%, P < 0.05 or P < 0.01). The percentage of S phase cells were 19.32% +/- 3.07%, 16.08% +/- 4.91% and 12.98% +/- 8.08% respectively, which were lower than that in control group (28.05% +/- 5.12%, P < 0.05 or P < 0.01). No obvious changes could be detected in 0.10, 0.25 mW/cm(2) microwave groups (P > 0.05). CONCLUSION: Microwave exceeding 0.50 mW/cm(2) may make injury to lens epithelial cells after 8 hour radiation, which may be related to the effect of microwave radiation on cell cycle.

AI evidence extraction

Main findings

After 8 h exposure at 2450 MHz, power densities of 0.50, 1.00, and 2.00 mW/cm^2 decreased proliferation, produced morphological changes (disordered arrangement, shrinkage, detachment), and were associated with increased G0/G1 phase and decreased S phase compared with controls (P<0.05 or P<0.01). No obvious changes were detected at 0.10 or 0.25 mW/cm^2 (P>0.05). The authors conclude that exposure exceeding 0.50 mW/cm^2 may injure lens epithelial cells after 8 h, potentially related to cell-cycle effects.

Outcomes measured

• Cell proliferation (MTT assay)
• Cell cycle distribution (PI fluorescent staining; G0/G1 and S phase percentages)
• Morphological changes (HE staining)
• DNA synthesis inhibition (as described)

Limitations

• In vitro study; findings may not translate to in vivo or human exposure
• Sample size not reported in abstract
• No SAR or dosimetry details beyond power density provided
• Exposure limited to a single duration (8 h)

{
    "study_type": "in_vitro",
    "exposure": {
        "band": "microwave",
        "source": null,
        "frequency_mhz": 2450,
        "sar_wkg": null,
        "duration": "8 h"
    },
    "population": "Cultured epithelial cells of rabbit lens (in vitro)",
    "sample_size": null,
    "outcomes": [
        "Cell proliferation (MTT assay)",
        "Cell cycle distribution (PI fluorescent staining; G0/G1 and S phase percentages)",
        "Morphological changes (HE staining)",
        "DNA synthesis inhibition (as described)"
    ],
    "main_findings": "After 8 h exposure at 2450 MHz, power densities of 0.50, 1.00, and 2.00 mW/cm^2 decreased proliferation, produced morphological changes (disordered arrangement, shrinkage, detachment), and were associated with increased G0/G1 phase and decreased S phase compared with controls (P<0.05 or P<0.01). No obvious changes were detected at 0.10 or 0.25 mW/cm^2 (P>0.05). The authors conclude that exposure exceeding 0.50 mW/cm^2 may injure lens epithelial cells after 8 h, potentially related to cell-cycle effects.",
    "effect_direction": "harm",
    "limitations": [
        "In vitro study; findings may not translate to in vivo or human exposure",
        "Sample size not reported in abstract",
        "No SAR or dosimetry details beyond power density provided",
        "Exposure limited to a single duration (8 h)"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "microwave radiation",
        "2450 MHz",
        "power density",
        "rabbit lens epithelial cells",
        "cell proliferation",
        "cell cycle",
        "MTT assay",
        "PI staining",
        "in vitro"
    ],
    "suggested_hubs": []
}

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