RF EMR exposure effects on amygdala morphology, place preference behavior and brain caspase-3 activity in rats

Abstract

RF EMR exposure effects on amygdala morphology, place preference behavior and brain caspase-3 activity in rats Narayanan, SN, Mohapatra, N, John, P, Suresh Kumar, NKR,. Nayak, SB, Gopalakrishna Bhat, P. Radiofrequency electromagnetic radiation exposure effects on amygdala morphology, place preference behavior and brain caspase-3 activity in rats. Environmental Toxicology and Pharmacology. 58:220-229. 2018. Abstract The purpose of the study was to evaluate the changes in amygdala morphology and emotional behaviors, upon exposure to chronic RF-EMR in adolescent rats. Four weeks old male albino Wistar rats were exposed to 900 MHz (power density:146.60 μW/cm2) from a mobile phone in silent-mode for 28 days. Amygdala morphology was studied using cresyl violet, TUNEL and Golgi-Cox staining. Place preference behavior was studied using light/dark chamber test and following this brain caspase-3 activity was determined. Number of healthy neurons was decreased in the basolateral amygdala and cortical amygdala but not in the central amygdala after RF-EMR exposure. It also induced apoptosis in the amygdala. RF-EMR exposure altered dendritic arborization pattern in basolateral amygdala but not in the central amygdala. Altered place preference and hyperactivity-like behavior was evident after RF-EMR exposure, but brain caspase-3 activity did not change. RF- EMR exposure perturbed normal cellular architecture of amygdala and this was associated with altered place preference. doi.org

AI evidence extraction

Main findings

Rats exposed to 900 MHz RF-EMR from a mobile phone for 28 days showed decreased numbers of healthy neurons in the basolateral and cortical amygdala (not central amygdala), evidence of apoptosis in the amygdala, and altered dendritic arborization in the basolateral amygdala (not central). Altered place preference and hyperactivity-like behavior were observed, while brain caspase-3 activity did not change.

Outcomes measured

• Amygdala morphology (cresyl violet staining)
• Apoptosis in amygdala (TUNEL)
• Dendritic arborization in amygdala (Golgi-Cox staining)
• Place preference / emotional behavior (light/dark chamber test)
• Brain caspase-3 activity

Suggested hubs

• mobile-phones (0.9)
  Exposure source described as a mobile phone (silent-mode) at 900 MHz.

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    "study_type": "animal",
    "exposure": {
        "band": "RF",
        "source": "mobile phone",
        "frequency_mhz": 900,
        "sar_wkg": null,
        "duration": "28 days (chronic exposure; started at 4 weeks of age)"
    },
    "population": "Four weeks old male albino Wistar rats (adolescent rats)",
    "sample_size": null,
    "outcomes": [
        "Amygdala morphology (cresyl violet staining)",
        "Apoptosis in amygdala (TUNEL)",
        "Dendritic arborization in amygdala (Golgi-Cox staining)",
        "Place preference / emotional behavior (light/dark chamber test)",
        "Brain caspase-3 activity"
    ],
    "main_findings": "Rats exposed to 900 MHz RF-EMR from a mobile phone for 28 days showed decreased numbers of healthy neurons in the basolateral and cortical amygdala (not central amygdala), evidence of apoptosis in the amygdala, and altered dendritic arborization in the basolateral amygdala (not central). Altered place preference and hyperactivity-like behavior were observed, while brain caspase-3 activity did not change.",
    "effect_direction": "harm",
    "limitations": [],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "radiofrequency electromagnetic radiation",
        "RF-EMR",
        "900 MHz",
        "mobile phone",
        "rats",
        "amygdala",
        "apoptosis",
        "TUNEL",
        "Golgi-Cox",
        "dendritic arborization",
        "place preference",
        "light/dark chamber",
        "caspase-3"
    ],
    "suggested_hubs": [
        {
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            "weight": 0.90000000000000002220446049250313080847263336181640625,
            "reason": "Exposure source described as a mobile phone (silent-mode) at 900 MHz."
        }
    ]
}

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