Effect of Luteolin on Biochemical, Immunohistochemical, and Morphometrical Changes in Rat Spinal Cord following Exposure to a 900 MHz Electromagnetic Field

PAPER manual 2020 Animal study Effect: harm Evidence: Low

Read original paper → DOI: 10.3967/bes2020.078 Evidence Lab

Abstract

Effect of Luteolin on Biochemical, Immunohistochemical, and Morphometrical Changes in Rat Spinal Cord following Exposure to a 900 MHz Electromagnetic Field Ahmad Yahyazadeh, Berrin Zuhal Altunkaynak. Effect of Luteolin on Biochemical, Immunohistochemical, and Morphometrical Changes in Rat Spinal Cord following Exposure to a 900 MHz Electromagnetic Field. Biomed Environ Sci. 2020 Aug 20;33(8):593-602. doi: 10.3967/bes2020.078. Abstract Objective: This study aimed to investigate the effect of exposure to a 900 MHz electromagnetic field (EMF) on the cervical spinal cord (CSC) of rats and the possible protective effect of luteolin (LUT) against CSC tissue damage. Methods: Quantitative data were obtained via stereological, biochemical, immunohistochemical, and histopathological techniques. We investigated morphometric value, superoxide dismutase (SOD) level, and the expression of high-mobility group box 1 protein molecules, as well as histological changes. Results: The total number of motor neurons in the EMF group significantly decreased in comparison with that in the control group ( P < 0.05). In the EMF + LUT group, we found a significant increase in the total number of motor neurons compared with that in the EMF group ( P < 0.05). SOD enzyme activity in the EMF group significantly increased in comparison with that in the control group ( P < 0.05). By contrast, the EMF+LUT group exhibited a decrease in SOD level compared with the EMF group ( P < 0.05). Conclusion: Our results suggested that exposure to EMF could be deleterious to CSC tissues. Furthermore, the protective efficacy of LUT against SC damage might have resulted from the alleviation of oxidative stress caused by EMF. Open access paper: besjournal.com

AI evidence extraction

At a glance

Study type

Animal study

Effect direction

harm

Population

Rats

Sample size

—

Exposure

RF · 900 MHz

Evidence strength

Low

Confidence: 78% · Peer-reviewed: yes

Main findings

Compared with controls, the EMF-exposed group had a significantly decreased total number of motor neurons and a significantly increased SOD activity. Compared with the EMF group, the EMF+LUT group showed a significant increase in motor neuron number and a decrease in SOD activity; authors conclude EMF exposure could be deleterious to CSC tissue and LUT may be protective.

Outcomes measured

Cervical spinal cord (CSC) morphometry / motor neuron number
Superoxide dismutase (SOD) enzyme activity
High-mobility group box 1 (HMGB1) expression
Histopathological / histological changes
Effect of luteolin (LUT) on EMF-associated changes

Limitations

Exposure duration not reported in abstract
SAR/dosimetry not reported in abstract
Sample size not reported in abstract
Randomization/blinding not described in abstract
Exposure source/setup not described in abstract

View raw extracted JSON

{
    "study_type": "animal",
    "exposure": {
        "band": "RF",
        "source": null,
        "frequency_mhz": 900,
        "sar_wkg": null,
        "duration": null
    },
    "population": "Rats",
    "sample_size": null,
    "outcomes": [
        "Cervical spinal cord (CSC) morphometry / motor neuron number",
        "Superoxide dismutase (SOD) enzyme activity",
        "High-mobility group box 1 (HMGB1) expression",
        "Histopathological / histological changes",
        "Effect of luteolin (LUT) on EMF-associated changes"
    ],
    "main_findings": "Compared with controls, the EMF-exposed group had a significantly decreased total number of motor neurons and a significantly increased SOD activity. Compared with the EMF group, the EMF+LUT group showed a significant increase in motor neuron number and a decrease in SOD activity; authors conclude EMF exposure could be deleterious to CSC tissue and LUT may be protective.",
    "effect_direction": "harm",
    "limitations": [
        "Exposure duration not reported in abstract",
        "SAR/dosimetry not reported in abstract",
        "Sample size not reported in abstract",
        "Randomization/blinding not described in abstract",
        "Exposure source/setup not described in abstract"
    ],
    "evidence_strength": "low",
    "confidence": 0.7800000000000000266453525910037569701671600341796875,
    "peer_reviewed_likely": "yes",
    "keywords": [
        "900 MHz",
        "electromagnetic field",
        "RF",
        "rat",
        "spinal cord",
        "cervical spinal cord",
        "motor neurons",
        "oxidative stress",
        "superoxide dismutase",
        "HMGB1",
        "luteolin",
        "histopathology",
        "immunohistochemistry",
        "stereology"
    ],
    "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract/metadata and may be incomplete or incorrect. This content is for informational purposes only and is not medical advice.

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