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Ameliorative Role of Coenzyme Q10 in RF Radiation-Associated Testicular and Oxidative Impairments in a 3.5-GHz Exposure Model

Research RF Safe Research Library Jan 1, 2026

This animal experiment assessed GSM-modulated 3.5 GHz RF exposure in male Wistar rats and reported hormonal, oxidative, and histological changes consistent with testicular impairment. RF exposure was associated with lower testosterone, LH, and FSH, higher oxidative stress (increased MDA and TOS), and degenerative testicular histology. Coenzyme Q10 supplementation partially mitigated several reported changes. The authors caution against generalizing these results to FR1 5G NR signals and call for further research.

The effects of acute and chronic exposure of 3G UMTS 2100 MHz radiofrequency radiation on rat mismatch negativity

Research RF Safe Research Library Jan 1, 2026

This rat study examined acute (1-week) and chronic (10-week) exposure to 2100 MHz radiofrequency radiation (3G UMTS-like) and assessed auditory mismatch negativity (MMN) alongside biochemical and histological brain measures. The abstract reports that acute exposure was associated with reduced MMN-related electrophysiological parameters and changes in GluR2 and GFAP with observed brain ultrastructural alterations. Chronic exposure showed opposite protein trends and enhanced MMN parameters versus chronic controls, and lipid peroxidation was not significantly different.

U.S. policy on wireless technologies and public health protection: regulatory gaps and proposed reforms

Research RF Safe Research Library Jan 1, 2025

This policy-focused paper contends that U.S. oversight of radiofrequency radiation from wireless technologies is outdated and insufficient, with exposure limits and testing approaches not aligned with modern long-term, chronic exposure scenarios. It emphasizes gaps in protections for children, pregnancy, vulnerable populations, workers, and wildlife, and describes limited monitoring, research, and enforcement capacity. The author proposes reforms to improve independent research, science-based limits, surveillance, and regulatory transparency.

Effects of paternal 5G RFR exposure on health of male offspring mice

Research RF Safe Research Library Jan 1, 2025

This animal study examined whether paternal exposure to 4.9 GHz (5G) radiofrequency radiation affects male offspring in C57BL/6 mice. It reports increased anxiety-like behavior and reduced sperm quality in adult F1 males from exposed fathers, alongside reported LRGUK hypermethylation and reduced LRGUK expression in testes. The abstract reports no significant effects on depression-like behavior, learning/memory, or fertility across F1–F2 generations.

Development and Testing of a Novel Whole-body Exposure System for Investigative Studies of Radiofrequency Radiation in Rodents (NIEHS)

Research RF Safe Research Library Jan 1, 2025

This NIEHS report describes the development and testing of a flexible whole-body radiofrequency radiation exposure system for rats and mice using updated signals relevant to wireless technologies. In 5-day studies with CDMA- and GSM-modulated signals, no visible behavioral responses were observed and comet assays reported no DNA damage in multiple tissues. The report notes technical challenges, particularly difficulty obtaining reliable body temperature measurements during exposure, and positions the system as a prototype for future mechanistic toxicology studies.

Radiofrequency radiation from mobile phones and the risk of breast cancer: A multicenter case-control study with an additional suspected comparison group

Research RF Safe Research Library Jan 1, 2025

This multicenter case-control study in Iran (n=226) examined associations between mobile phone use and breast cancer outcomes in women. Reporting more than 60 minutes/day of phone conversations was associated with higher odds of confirmed invasive breast cancer and of being classified as a suspected case versus <10 minutes/day. The authors emphasize that the results do not establish causation and may be influenced by self-reported exposure and residual confounding, warranting cautious interpretation.

Radiofrequency radiation-induced gene expression

Research RF Safe Research Library Jan 1, 2025

This review summarizes studies reporting radiofrequency radiation (RFR)-associated changes in gene expression across biological systems. Reported affected genes relate to cellular stress responses, oxidative processes, apoptosis, DNA damage detection/repair, protein repair, and neural function regulation. The authors highlight reported gene expression effects at or below 0.4 W/kg SAR and argue this challenges current guideline assumptions, while noting that not all studies find significant effects.

Male Reproductive and Cellular Damage After Prenatal 3.5 GHz Radiation Exposure: One-Year Postnatal Effects

Research RF Safe Research Library Jan 1, 2025

This animal study examined whether prenatal exposure to 3.5 GHz radiofrequency radiation (2 hours/day) affects male reproductive outcomes later in life. Male rat offspring assessed at 12 months showed multiple adverse testicular and cellular findings in exposed groups versus sham controls, including impaired spermatogenesis markers, increased abnormal sperm morphology, increased DNA damage, and increased apoptosis, with full-gestation exposure generally most pronounced. The authors interpret the results as evidence of persistent reproductive toxicity from prenatal exposure and call for further mechanistic work and precautionary actions.

The WHO-commissioned systematic reviews on health effects of radiofrequency radiation provide no assurance of safety

Research RF Safe Research Library Jan 1, 2025

This paper evaluates and critiques 12 WHO-commissioned systematic reviews and meta-analyses on RF-EMF health effects across outcomes including cancer and reproductive endpoints. It argues that serious methodological flaws and limitations in the WHO reviews prevent them from providing assurance of safety for cell phones and other wireless devices. The authors highlight reported evidence in the animal cancer review (high certainty for heart schwannomas; moderate certainty for brain gliomas) and describe dose-related adverse effects on male fertility and reproductive outcomes, including at exposure levels below current ICNIRP thresholds.

Carcinogenicity of Radio-Frequency Radiation: Similarities and Differences Between Outcomes of Two Studies

Research RF Safe Research Library Jan 1, 2025

This review compares two epidemiologic studies of radio-frequency radiation (RFR) exposure among military personnel with overlapping patient groups. One study reported a statistically significant increase in cancer among exposed individuals, while the other did not, which the review attributes to a smaller sample size. The review highlights similar cancer patterns across both studies, including a high proportion of hematolymphoid cancers and earlier onset among exposed individuals, and interprets these similarities as evidence of carcinogenic effects.

Protective effects of quercetin against 3.5 GHz RF radiation-induced thyroid dysfunction and oxidative stress in rats

Research RF Safe Research Library Jan 1, 2025

This rat study examined repeated 3.5 GHz RF exposure (2 hours/day, 5 days/week for 30 days) and thyroid-related outcomes, with and without quercetin. The abstract reports altered thyroid hormones (lower T3/T4, higher TSH) and increased oxidative stress in thyroid tissue after RF exposure. Quercetin appeared partially protective, though effects were not uniformly statistically significant, and SAR simulations indicated relatively higher absorption in the thyroid region.

The effects of short-term and long-term 2100 MHz radiofrequency radiation on adult rat auditory brainstem response

Research RF Safe Research Library Jan 1, 2025

This animal study examined 2100 MHz radiofrequency radiation exposure effects on auditory brainstem responses and brain oxidative/ultrastructural markers in adult rats. The 1-week exposure group showed prolonged ABR latencies and biochemical/structural changes consistent with oxidative stress and cellular injury. The authors report no harmful effects in the 10-week exposure condition with rest days under the studied protocol.

Exploring the impact of environmental factors on male reproductive health through epigenetics

Research RF Safe Research Library Jan 1, 2025

This narrative review discusses how environmental factors may affect male reproductive health through epigenetic mechanisms, including DNA methylation, histone modifications, chromatin remodeling, and non-coding RNA regulation. It reports that electromagnetic radiation, particularly from mobile phones and wireless devices, is linked in the reviewed literature to reduced sperm count and motility, increased oxidative stress, and chromatin damage. The authors conclude there is a substantive connection between EMF exposure and adverse male reproductive outcomes and suggest practical risk-reduction guidance.

A Mini-Review of the Potential Health Impacts of Indoor Radiation Exposure in Companion Animals

Research RF Safe Research Library Jan 1, 2025

This mini-review discusses indoor radiation sources that may affect companion animals, including radon, radionuclides in feed, radiofrequency sources (phones, Wi-Fi, pet tracking devices), solar radiation, and extremely low frequency radiation. It reports that indoor radiation may negatively impact companion animal health and well-being. The authors conclude that preventive and precautionary measures are necessary to protect companion animals from indoor radiation exposure.

Understanding Electromagnetic Hypersensitivity (EHS) From Mobile Phone Radiofrequency Radiation (RFR) Exposure: A Mixed-Method Study Protocol

Research RF Safe Research Library Jan 1, 2025

This paper presents a mixed-method study protocol examining electromagnetic hypersensitivity (EHS) in relation to mobile phone radiofrequency radiation exposure among undergraduate students. The quantitative component aims to identify predictors of EHS using a biopsychosocial model, while the qualitative component explores individual experiences through in-depth interviews. The abstract provides study design details and sample size but does not report study results.

High Radiofrequency Radiation in the Surroundings of 10 Schools in Örebro, Sweden

Research RF Safe Research Library Jan 1, 2025

This exposure assessment measured outdoor RF radiation from 4G/5G base stations near 10 schools in Örebro, Sweden (October 2024). The authors report maximum levels of 10,716–68,452 μW/m² and state these are far above EUROPAEM EMF guideline ranges; two locations also showed higher peak readings with a second meter. The paper frames these findings as indicating a significant health risk, particularly for children and sensitive groups, though no health outcomes were measured in this study.

Effects of 700MHz radiofrequency radiation (5G lower band) on the reproductive parameters of female Wistar rats

Research RF Safe Research Library Jan 1, 2025

This animal study examined short- and long-term 700 MHz (lower-band 5G) radiofrequency exposure in female Wistar rats, comparing control, sham, and exposed groups. It reports no DNA damage and no change in estrous cycle length, but increased ovarian oxidative stress markers in exposed animals. Long-term exposure was associated with ovarian histopathological alterations, while estradiol and progesterone stayed within normal ranges and testosterone increased slightly but significantly.

The effects of radiofrequency radiation on male reproductive health and potential mechanisms (Review)

Research RF Safe Research Library Jan 1, 2025

This narrative review summarizes human and animal research on radiofrequency (RF) radiation exposure (e.g., mobile phones, Wi‑Fi, occupational sources) and male reproductive outcomes. It reports that the literature links RF exposure with reduced sperm quality and increased DNA damage, often alongside oxidative stress and other proposed biological changes. Although inconsistencies are acknowledged, the authors conclude the overall evidence suggests harmful associations and call for standardized, long-term studies and reconsideration of guidelines.

Use of Mobile and Cordless Phones and the Association with Prostate Cancer

Research RF Safe Research Library Jan 1, 2025

This pooled analysis of two prior human studies reports increased odds of prostate cancer associated with mobile phone use, with higher estimates for longer latency and higher cumulative hours. Cordless phone use is reported to show increased risk but not statistically significant overall, with one mid-range cumulative use category showing an elevated OR. The abstract also reports higher risks among men with more aggressive prostate cancer and among those with a family history of prostate cancer.

The CB1R of mPFC is involved in anxiety-like behavior induced by 0.8/2.65 GHz dual-frequency electromagnetic radiation

Research RF Safe Research Library Jan 1, 2025

This animal study reports that dual-frequency RF EMR exposure (0.8/2.65 GHz, 4 W/kg) induced anxiety-like behavior in mice. It also reports reduced CB1R and endocannabinoid levels in the mPFC and altered endocannabinoid system markers in the BLA. CB1R overexpression or knockdown in the mPFC reportedly decreased or increased anxiety-like behavior, respectively, suggesting a mechanistic link in this model.

Is Cellphone Carrying Below the Waist (Exposure to Non-Ionizing Radiation) Contributing to the Rapid Rise in Early-Onset Colorectal Cancer?

Research RF Safe Research Library Jan 1, 2024

This conference abstract reports a pilot matched case-control study examining whether carrying a cellphone below the waist is associated with early-onset colorectal cancer (EOCRC). The authors report higher EOCRC likelihood among those who carried phones below the waist, with the strongest association for ipsilateral carrying (same side as the tumor) and high cumulative hours. Details on exposure measurement, confounding control, and full statistical reporting are not provided in the abstract.

Evaluation of the Thyroids of Offsprings Exposed to 2450 MHz Radiofrequency Radiation During Pregnancy: A Sixth Month Data

Research RF Safe Research Library Jan 1, 2024

This animal study examined whether continuous prenatal exposure to 2450 MHz radiofrequency radiation (simulating Wi‑Fi) affects thyroid tissue in rat offspring at 6 months. The exposed group showed significantly increased mononuclear cell infiltration and vascular congestion in thyroid histology. TUNEL-positive cell percentage and H2A.X antibody levels did not differ significantly between groups.

Electromagnetic fields and DNA damage

Research RF Safe Research Library Jan 1, 2009

This review discusses the comet assay and summarizes research on non-ionizing EMF exposure and DNA/chromosomal damage. It describes both positive and negative findings across studies, noting no consistent overall pattern for radiofrequency radiation (RFR). The authors nonetheless conclude that under certain exposure conditions RFR appears genotoxic and may affect DNA damage and repair, with evidence discussed as most applicable to exposures typical of cell phone use.

Cell phones and brain tumors: a review including the long-term epidemiologic data

Research RF Safe Research Library Jan 1, 2009

This paper presents a meta-analysis of 11 peer-reviewed epidemiologic studies examining long-term (>=10 years) cell phone use with laterality analyses. It reports that long-term use is associated with an approximately doubled risk of an ipsilateral brain tumor. The abstract states statistical significance for glioma and acoustic neuroma, but not for meningioma.

Genetic damage in mammalian somatic cells exposed to radiofrequency radiation: a meta-analysis of data from 63 publications (1990-2005)

Research RF Safe Research Library Jan 1, 2008

A meta-analysis of 63 publications assessed whether radiofrequency (RF) radiation exposure is associated with genetic damage in mammalian somatic cells using multiple genotoxicity endpoints. Overall differences between RF-exposed and control conditions were reported as small, though statistically significant increases were observed for some endpoints under certain exposure conditions. Mean chromosomal aberration and micronucleus indices were reported to fall within historical spontaneous levels, and the analysis found considerable evidence of publication bias.

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