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Extremely Low-Frequency Electromagnetic Fields Increase Cytokines in Human Hair Follicles through Wnt/β-Catenin Signaling.

Research RF Safe Research Library Jan 1, 2022

This in vitro/ex vivo study examined extremely low-frequency EMF exposure in human hair bulb spheroids and hair follicle organ cultures. The authors report no toxicity and increased expression of anagen-related markers and multiple cytokines/growth factors at 5–20 G, with 10 G for 60 minutes associated with increased…

Changes in Free Radical Processes under the Influence of Low-Frequency Electromagnetic Field in Rats.

Research RF Safe Research Library Jan 1, 2022

This in vivo animal study examined how low-frequency electromagnetic field exposure affects antioxidant status in Wistar rats. The authors report frequency-dependent changes in antioxidant enzyme activity and hydroperoxide content in blood plasma. They also propose that LF EMF may stimulate low levels of ROS that…

Physiological changes and symptoms associated with short-term exposure to electromagnetic fields: a randomized crossover provocation study.

Research RF Safe Research Library Jan 1, 2022

This randomized blinded crossover provocation study compared short-term RF-EMF exposure mimicking mobile phone base stations versus sham in people with IEI-EMF and controls. Symptom reporting and physiological measures (blood pressure, heart rate, heart rate variability) were similar between provocation and sham…

Treatment with Pulsed Extremely Low Frequency Electromagnetic Field (PELF-EMF) Exhibit Anti-Inflammatory and Neuroprotective Effect in Compression Spinal Cord Injury Model.

Research RF Safe Research Library Jan 1, 2022

This animal study tested a pulsed extremely low frequency electromagnetic field (PELF-EMF) SEQEX device in a mouse compression spinal cord injury model. Mice received 4 hours of daily exposure for two months starting 2 hours after injury. The study reports reduced inflammatory and astrocyte activation markers,…

Physiotherapeutic Reduction of Orofacial Pain Using Extremely Low-Frequency Electromagnetic Field and Light-Emitting Diode Therapy-A Pilot Study.

Research RF Safe Research Library Jan 1, 2022

This randomized pilot study evaluated whether adding combined extremely low-frequency electromagnetic field (ELF EMF) and LED physiotherapy to standard analgesics reduces postoperative orofacial pain after orthognathic surgery. The physiotherapy group reportedly experienced faster pain reduction than controls. The…

Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined how low-frequency electromagnetic fields (LF-EMF) at different magnetic flux densities influence skeletal myogenesis in C2C12 cells. The authors report that 2.0 mT LF-EMF activated cells and increased PAX7, while 1.5 mT increased MyoD and myogenin expression. They conclude that different…

Anticancer and antibacterial potentials induced post short-term exposure to electromagnetic field and silver nanoparticles and related pathological and genetic alterations: in vitro study.

Research RF Safe Research Library Jan 1, 2022

This in vitro study evaluated short-term extremely low frequency electromagnetic field (ELF-EMF) exposure and silver nanoparticles (AgNPs), alone and combined, for antibacterial effects and apoptosis induction in MCF-7 breast cancer cells. The authors report time-dependent reductions in bacterial viable counts, with…

Cancer on-target: Selective enhancement of 3-bromopyruvate action by an electromagnetic field in vitro.

Research RF Safe Research Library Jan 1, 2022

This in vitro study tested continuous or pulsed ELF electromagnetic field exposure in human cancer cells treated with the anticancer agent 3-bromopyruvate (3BP). The authors report that ELF-EMF enhanced 3BP anti-cancer activity, which they link to reduced TNFα secretion, p21/p27-associated G2/M arrest, and cancer…

Health disturbances and exposure to radiofrequency electromagnetic fields from mobile-phone base stations in French urban areas.

Research RF Safe Research Library Jan 1, 2021

This cross-sectional survey in five French cities assessed whether measured RF-EMF exposure from mobile-phone base stations was associated with self-reported non-specific and insomnia-like symptoms among residents living within 250 m of a base station. Overall, measured base-station exposure was not associated with…

The Short-Term Effect of Occupational Levels of 50 Hz Electromagnetic Field on Human Heart Rate Variability.

Research RF Safe Research Library Jan 1, 2021

This double-blind randomized sham-controlled repeated-measures study examined short-term 50 Hz ELF-EMF exposure (28 μT) to the chest and its effects on HR and HRV in 34 healthy young men. HR deceleration occurred in both EMF and sham conditions, but several HRV indices (SDNN, RMSSD, LF, HF) increased only in the EMF…

Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…

Comparing the Effects of Long-term Exposure to Extremely Low-frequency Electromagnetic Fields With Different Values on Learning, Memory, Anxiety, and β-amyloid Deposition in Adult Rats.

Research RF Safe Research Library Jan 1, 2021

This animal study exposed adult male rats to 50 Hz ELF-EMF at 1, 100, 500, or 2000 μT for 1 hour/day over two months and assessed behavior and brain markers. The authors report increased memory retention (higher step-through latency) alongside increased anxiety-like behavior (reduced open-arm entries), particularly…

Artificial Neural Network-Based Uplink Power Prediction From Multi-Floor Indoor Measurement Campaigns in 4G Networks.

Research RF Safe Research Library Jan 1, 2021

This paper describes multi-floor indoor LTE measurement campaigns aimed at enabling assessment and monitoring of RF-EMF exposure, with emphasis on uplink exposure via mobile phone transmit power. Because uplink transmit power is not directly available from off-the-shelf devices, the authors propose an artificial…

EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.

Research RF Safe Research Library Jan 1, 2021

This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…

The Time-Dependence of Three Different Modes of ELF-EMF Stimulation on LTP at Schaffer Collateral-CA1 Synapses.

Research RF Safe Research Library Jan 1, 2021

This animal ex vivo study tested whether short-term 15 Hz/2 mT ELF-EMF exposure alters LTP at rat hippocampal SC-CA1 synapses. Across sinusoidal, pulsed, and rhythm-pulsed modes, the authors report reduced LTP versus control, with greater inhibition at longer exposure durations (10–60 s). Sinusoidal stimulation…

Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…

Necroptosis triggered by ROS accumulation and Ca overload, partly explains the inflammatory responses and anti-cancer effects associated with 1Hz, 100 mT ELF-MF in vivo.

Research RF Safe Research Library Jan 1, 2021

This animal and in vitro study examined ELF magnetic field exposure in a mouse breast tumor model and a breast cancer cell line. The authors report tumor growth suppression and reduced expression of Ki-67, CD31, VEGFR2, and MMP-9 after exposure. They also report increased IFN-γ and TNF-α and increased…

Electromagnetic Field (EMF) Radiation Alters Estrogen Release from the Pig Myometrium during the Peri-Implantation Period.

Research RF Safe Research Library Jan 1, 2021

This ex vivo animal-tissue study tested whether ELF-EMF exposure (50 or 120 Hz for 2 or 4 hours) alters estrogenic activity in pig myometrium during the peri-implantation period. The authors report EMF-associated changes in estrogen-related gene transcripts and aromatase abundance, with progesterone diminishing some…

Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2021

This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…

Effect of extremely low frequency electromagnetic field parameters on the proliferation of human breast cancer.

Research RF Safe Research Library Jan 1, 2021

This in vitro study tested how varying ELF-EMF parameters (frequency, flux density, duration, and continuous vs switching exposure) affect breast cancer and normal breast epithelial cell lines. The authors report that an optimized switching exposure pattern (7.83 ± 0.3 Hz, 1 mT) reduced viability of MDA-MB-231 cells…

Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.

Research RF Safe Research Library Jan 1, 2021

This in vitro study examined whether low-frequency electromagnetic field (LF-EMF) exposure affects viability of human oral mucosa keratinocytes. Apoptosis (early/late) and necrosis were measured by flow cytometry, including conditions with lipopolysaccharide (LPS) or minocycline. The authors report that LF-EMF, alone…

Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia.

Research RF Safe Research Library Jan 1, 2021

This animal study evaluated whether ELF-EMF exposure affects recovery-related outcomes after cerebral ischemia in rats. Rats exposed to 50 Hz, 1 mT ELF-EMF for 2 hours daily over 28 days showed better Morris water maze performance than untreated rats. The study also reports increased hippocampal neurogenesis markers…

[Jurisprudence on occupational radiofrequency diseases].

Research RF Safe Research Library Jan 1, 2020

This article reviews Italian jurisprudence on alleged occupational tumours linked to RF EMF exposure (notably from wireless phones), with some cases also involving other RF sources or ELF magnetic fields. It contrasts court decisions that recognized occupational causation with others that rejected it, emphasizing how…

Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells.

Research RF Safe Research Library Jan 1, 2020

This in vitro study examined 50 Hz, 1 mT ELF-EMF exposure in LPS-treated THP-1 cells for 1–24 hours. The authors report increased HO-1 expression and Nrf2-related signaling, with nuclear enrichment of HO-1 and modulation of SIRT1/NF-κB pathways. They interpret these molecular changes as suggesting a protective,…

Electromagnetic field affects the voltage-dependent potassium channel Kv1.3.

Research RF Safe Research Library Jan 1, 2020

This in vitro study tested whether extremely low-frequency electromagnetic fields (ELF-EMF) modulate the voltage-gated potassium channel Kv1.3 in Kv1.3-expressing CHO-K1 cells. Cells were exposed to a 20 Hz field at 268 µT or 902 µT while Kv1.3 currents were measured by whole-cell patch clamp. The authors report a…

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