Effect of rmEGF combined with ELF-EMF on promoting wound healing in rats.
Research
RF Safe Research Library
Jan 1, 2024
This randomized animal study evaluated whether recombinant murine EGF combined with ELF-EMF improves wound healing in rats. The combined ELF-EMF+rmEGF group showed better wound healing rates and more favorable histological features than comparison groups at multiple time points. All groups had complete wound closure…
Exposure to the extremely low-frequency electromagnetic field induces changes in the epigenetic regulation of gene expression in the endometrium.
Research
RF Safe Research Library
Jan 1, 2024
This in vitro study exposed porcine endometrial slices from days 15–16 of pregnancy to a 50 Hz extremely low-frequency electromagnetic field for 2 hours. The authors report changes in several epigenetic-related markers, including DNA methylation machinery, histone-related measures (HDAC activity), global DNA…
Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study tested whether ELF-EMF exposure (50 Hz, 1 mT; 30 min/day) influences osteogenic differentiation markers in rat bone-marrow-derived stem cells cultured in osteogenic medium or in human adipose-stem cell-conditioned medium. The OM + EMF condition showed higher ALP activity and higher Runx2 and Ocn…
The Effect of Extremely Low-Frequency Electromagnetic Fields on Inflammation and Performance-Related Indices in Trained Athletes: A Double-Blinded Crossover Study.
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RF Safe Research Library
Jan 1, 2023
This double-blinded crossover study tested an ELF-EMF mattress used immediately after high-intensity cycling and overnight in nine trained male athletes. The authors report no significant overall effects on performance and inflammation. However, between 60 minutes and 24 hours post-exercise, the active condition…
P53 status, and G2/M cell cycle arrest, are determining factors in cell-death induction mediated by ELF-EMF in glioblastoma.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study tested ELF-EMF exposure in U87 and U251 glioblastoma cell lines and assessed apoptosis, cell-cycle distribution, cell size, and mRNA expression of cell-cycle genes. The authors report higher apoptosis induction and increased G2/M arrest in U87 compared with U251, alongside increased U87 cell size…
Extremely low-frequency electromagnetic field exposure alters DNA methylation levels in the endometrium of pigs during the peri-implantation period.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study exposed porcine endometrial slices from the peri-implantation period (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed promoter DNA methylation in selected genes. The authors report increased methylation for EGR2, ID2, and PTGER4, decreased methylation for IL1RAP and NOS3, and no…
Synergistic cytotoxic effects of an extremely low-frequency electromagnetic field with doxorubicin on MCF-7 cell line.
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Jan 1, 2023
This in vitro study tested whether a 50 Hz, 20 mT extremely low-frequency electromagnetic field (ELF-EMF) enhances the cytotoxic effects of doxorubicin (DOX) in the MCF-7 breast cancer cell line. The authors report that combining ELF-EMF with low-dose DOX reduced cell viability at lower DOX concentrations than DOX…
Measuring and assessing the effects of extremely low-frequency electromagnetic fields (ELF-EMF) on blood parameters and liver enzymes of personnel working in high voltage power stations in a petrochemical industry.
Research
RF Safe Research Library
Jan 1, 2023
This cross-sectional study in a petrochemical industry compared 50 high-voltage power-station workers exposed to ELF-EMF with 50 controls. Electric and magnetic fields were measured at 200 points, and blood parameters and liver enzymes were extracted from medical records. The study reports no significant differences…
Impact of ketamine administration on chronic unpredictable stress-induced rat model of depression during extremely low-frequency electromagnetic field exposure: Behavioral, histological and molecular study.
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Jan 1, 2023
This animal study evaluated ketamine administration combined with extremely low-frequency electromagnetic field (ELF-EMF) exposure in a chronic unpredictable stress (CUS) rat model. The authors report that the combined treatment increased depression-like behavior and neuronal degeneration, reduced GFAP(+) cells in…
Effect of extremely low frequency electromagnetic field on the pathogenicity of Magnaporthe oryzae.
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Jan 1, 2023
This laboratory study examined Magnaporthe oryzae pathogenicity under extremely low frequency electromagnetic field exposure (50 Hz, 3 mT). The authors report increased infection ability, growth rate, and conidia generation with ELF-EMF exposure. They also report increased tolerance to a cell wall-perturbing agent…
Effects of repeated exposure to 50 Hz electromagnetic field on breast cancer cells.
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Jan 1, 2022
This in vitro study exposed two human breast cancer cell lines to a 50 Hz ELF-EMF (20 mT) for 3 hours/day for up to four days. The authors report reduced viability and apoptosis-related findings in MDA-MB-231 cells, with no significant viability change in MCF-7 cells. Cell-cycle effects differed by cell line,…
Cone Beam Computed Tomography in the Assessment of the Effectiveness of Physical Therapy with the Use of the Electromagnetic Field Combined with Light Radiation Emitted by LEDs in the Treatment of Inflammation of the Paranasal Sinuses-A Case Study.
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Jan 1, 2022
This case study used cone beam computed tomography (CBCT) to assess a physical therapy approach combining extremely low frequency electromagnetic field (ELF-EMF) exposure with LED light for paranasal sinusitis. In one 39-year-old female patient, CBCT findings reportedly showed near-complete restoration of sinus…
Effects of prolonged exposure to ELF-EMF on HERVs expression in human melanoma cells.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined whether 96-hour exposure to 50 Hz ELF-EMF affects expression of human endogenous retroviruses (HERV-H, HERV-K, HERV-W) in SK-MEL-37 human melanoma cells. Cells were continuously exposed at 1.5 or 3 mT and HERV env mRNA levels were measured by qPCR. The authors report significant…
Thermomagnetic Resonance Effect of the Extremely Low Frequency Electromagnetic Field on Three-Dimensional Cancer Models.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study tested an ELF-EMF at a calculated thermal resonant frequency in 2D and 3D culture models of human pancreatic cancer, glioblastoma, and breast cancer. The authors report reduced cell growth and a metabolic shift, including potentiation of coupled and uncoupled respiration and altered transcription…
Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering.
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Jan 1, 2022
This in vitro study examined intermittent extremely low-frequency electromagnetic field (ELF-EMF) exposure (6 h/day) in human periodontal ligament mesenchymal stem cells during osteogenic differentiation. After 10 days, ELF-EMF-exposed cells showed increased proliferation and calcium deposition versus sham.…
Bidirectional Effect of Repeated Exposure to Extremely Low-Frequency Electromagnetic Field (50 Hz) of 1 and 7 mT on Oxidative/Antioxidative Status in Rat's Brain: The Prediction for the Vulnerability to Diseases.
Research
RF Safe Research Library
Jan 1, 2022
This rat study examined repeated 50 Hz ELF-EMF exposure (1 or 7 mT) and measured oxidative stress and antioxidant defense markers in the prefrontal cortex, including responses to a subsequent open-field stressor. The authors report intensity- and repetition-dependent effects: 1 mT produced weak changes and was…
Extremely Low-Frequency Electromagnetic Fields Increase Cytokines in Human Hair Follicles through Wnt/β-Catenin Signaling.
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Jan 1, 2022
This in vitro/ex vivo study examined extremely low-frequency EMF exposure in human hair bulb spheroids and hair follicle organ cultures. The authors report no toxicity and increased expression of anagen-related markers and multiple cytokines/growth factors at 5–20 G, with 10 G for 60 minutes associated with increased…
Treatment with Pulsed Extremely Low Frequency Electromagnetic Field (PELF-EMF) Exhibit Anti-Inflammatory and Neuroprotective Effect in Compression Spinal Cord Injury Model.
Research
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Jan 1, 2022
This animal study tested a pulsed extremely low frequency electromagnetic field (PELF-EMF) SEQEX device in a mouse compression spinal cord injury model. Mice received 4 hours of daily exposure for two months starting 2 hours after injury. The study reports reduced inflammatory and astrocyte activation markers,…
Anticancer and antibacterial potentials induced post short-term exposure to electromagnetic field and silver nanoparticles and related pathological and genetic alterations: in vitro study.
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Jan 1, 2022
This in vitro study evaluated short-term extremely low frequency electromagnetic field (ELF-EMF) exposure and silver nanoparticles (AgNPs), alone and combined, for antibacterial effects and apoptosis induction in MCF-7 breast cancer cells. The authors report time-dependent reductions in bacterial viable counts, with…
Cancer on-target: Selective enhancement of 3-bromopyruvate action by an electromagnetic field in vitro.
Research
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Jan 1, 2022
This in vitro study tested continuous or pulsed ELF electromagnetic field exposure in human cancer cells treated with the anticancer agent 3-bromopyruvate (3BP). The authors report that ELF-EMF enhanced 3BP anti-cancer activity, which they link to reduced TNFα secretion, p21/p27-associated G2/M arrest, and cancer…
The Short-Term Effect of Occupational Levels of 50 Hz Electromagnetic Field on Human Heart Rate Variability.
Research
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Jan 1, 2021
This double-blind randomized sham-controlled repeated-measures study examined short-term 50 Hz ELF-EMF exposure (28 μT) to the chest and its effects on HR and HRV in 34 healthy young men. HR deceleration occurred in both EMF and sham conditions, but several HRV indices (SDNN, RMSSD, LF, HF) increased only in the EMF…
Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.
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RF Safe Research Library
Jan 1, 2021
This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…
Comparing the Effects of Long-term Exposure to Extremely Low-frequency Electromagnetic Fields With Different Values on Learning, Memory, Anxiety, and β-amyloid Deposition in Adult Rats.
Research
RF Safe Research Library
Jan 1, 2021
This animal study exposed adult male rats to 50 Hz ELF-EMF at 1, 100, 500, or 2000 μT for 1 hour/day over two months and assessed behavior and brain markers. The authors report increased memory retention (higher step-through latency) alongside increased anxiety-like behavior (reduced open-arm entries), particularly…
EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…
The Time-Dependence of Three Different Modes of ELF-EMF Stimulation on LTP at Schaffer Collateral-CA1 Synapses.
Research
RF Safe Research Library
Jan 1, 2021
This animal ex vivo study tested whether short-term 15 Hz/2 mT ELF-EMF exposure alters LTP at rat hippocampal SC-CA1 synapses. Across sinusoidal, pulsed, and rhythm-pulsed modes, the authors report reduced LTP versus control, with greater inhibition at longer exposure durations (10–60 s). Sinusoidal stimulation…