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Showing results for: in vitro

Nanosecond pulse electric field activation of platelet-rich plasma reduces myocardial infarct size and improves left ventricular mechanical function in the rabbit heart.

Research RF Safe Research Library Jan 1, 2012

This animal and in vitro study evaluated nanosecond pulsed electric fields (nsPEF) as a nonchemical method to activate platelet-rich plasma (PRP) for cardioprotection. In rabbits undergoing brief coronary occlusion followed by reperfusion, PRP injection into ischemic myocardium was associated with reduced infarct…

[Effects of static magnetic field at different times on the proliferation and differentiation of osteoblasts in vitro].

Research RF Safe Research Library Jan 1, 2012

This in vitro study exposed newborn rat calvarial osteoblasts to a 3.9 mT static magnetic field for varying durations (0–4 hours). The authors report increased cell proliferation and markers of osteoblast differentiation and mineralization versus control, including higher ALP activity, calcium content, and…

Evaluation of the packaging and encapsulation reliability in fully integrated, fully wireless 100 channel Utah Slant Electrode Array (USEA): Implications for long term functionality.

Research RF Safe Research Library Jan 1, 2012

This engineering study evaluated long-term encapsulation and packaging reliability of a fully wireless 100-channel Utah Slant Electrode Array system in vitro. The device was soaked in PBS for over 12 months while being wirelessly powered/configured at 2.765 MHz and transmitting an FSK-modulated 900 MHz RF signal. The…

New insights into bioprotective effectiveness of disaccharides: an FTIR study of human haemoglobin aqueous solutions exposed to static magnetic fields.

Research RF Safe Research Library Jan 1, 2012

This in vitro FTIR study examined human haemoglobin aqueous solutions exposed to a 200 mT static magnetic field for 3 and 7 hours, with and without the disaccharides sucrose and trehalose. The authors report spectral changes (amide A decrease and reduced CH(3) stretching) in haemoglobin dissolved in bi-distilled…

Electric Field Controlled Self-Assembly of Hierarchically Ordered Membranes.

Research RF Safe Research Library Jan 1, 2012

This in vitro study examined how an externally applied electric field influences the self-assembly of oppositely charged molecules in water into an ordered membrane. The authors report that electric fields changed the kinetics of membrane formation and altered membrane thickness, nanofiber growth orientation, and…

Low-frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines.

Research RF Safe Research Library Jan 1, 2012

This in vitro study examined whether low-frequency electromagnetic fields (a complex multiple waveform field and a 50 Hz sine wave) alter inflammatory gene and protein responses in human immune cells. Across time course experiments in THP-1 cells and primary monocytes/macrophages, the authors report no significant…

[Time effect of sinusoidal electromagnetic field on enhancing the maturation and mineralization of osteoblasts in vitro].

Research RF Safe Research Library Jan 1, 2011

This in vitro study exposed newborn rat calvarial osteoblasts to 50 Hz sinusoidal electromagnetic fields at 1.8 mT for different daily exposure times (0.5–3.0 h). The authors report inhibited osteoblast proliferation at longer exposure times, alongside increased differentiation markers (ALP activity) and…

Human keratinocytes in culture exhibit no response when exposed to short duration, low amplitude, high frequency (900 MHz) electromagnetic fields in a reverberation chamber.

Research RF Safe Research Library Jan 1, 2011

This in vitro study exposed normal human epidermal keratinocytes to non-thermal 900 MHz electromagnetic fields in a mode-stirred reverberation chamber under two short-duration conditions. Microarray analysis found only limited gene modulation (20 genes) with small expression ratios and no shared genes across…

Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2011

This in vitro study examined whether extremely low-frequency magnetic fields affect chondrogenic differentiation of human mesenchymal stem cells in pellet culture. Cells exposed for 3 weeks to a homogeneous sinusoidal 5 mT magnetic field showed increased collagen type II expression (significant at passage 6) and…

In vitro tests reveal sample radiofrequency identification readers inducing clinically significant electromagnetic interference to implantable pacemakers and implantable cardioverter-defibrillators.

Research RF Safe Research Library Jan 1, 2010

This in vitro study tested electromagnetic compatibility between passive RFID readers and 15 pacemakers plus 15 ICDs across 134 kHz, 13.56 MHz, and 915 MHz bands. EMI reactions were common with low-frequency RFID and rare with high-frequency RFID, while no reactions were observed with ultra-high-frequency or…

In vitro magnetic stimulation of pig phrenic nerve with transverse and longitudinal induced electric fields: analysis of the stimulation site.

Research RF Safe Research Library Jan 1, 2009

This in vitro study analyzed where magnetic stimulation activates pig phrenic nerves when induced electric fields are oriented longitudinally versus transversely to the nerve trunk. Longitudinal-field stimulation localized activation near the negative peak of the spatial derivative of the longitudinal electric field,…

Role of cortical cell type and morphology in subthreshold and suprathreshold uniform electric field stimulation in vitro.

Research RF Safe Research Library Jan 1, 2009

This in vitro study examined how cortical neuron morphology and cell type relate to responses under subthreshold and suprathreshold uniform electric fields in rat motor cortex slices. The authors report that morphology correlates with subthreshold somatic polarization and that layer V pyramidal neurons are predicted…

[Effects of 50 Hz sinusoidal magnetic field on Ca2+ release channel ryanodine receptor of sarcoplasmic reticulum vesicles].

Research RF Safe Research Library Jan 1, 2009

This in vitro study examined whether a 50 Hz sinusoidal magnetic field affects the sarcoplasmic reticulum calcium release channel RyR1. A 0.4 mT exposure for 30 minutes increased SR Ca2+ release rate and [(3)H]-ryanodine binding under NADH/NAD+ conditions. The authors also report increased NADH oxidation and…

[Effect of microwave radiation on primary cultured Sertoli cells].

Research RF Safe Research Library Jan 1, 2009

This in vitro study exposed primary cultured Sertoli cells to microwave radiation at 0, 30, or 100 mW/cm^2 for 5 minutes and assessed outcomes 6 hours later. The authors report no differences in apoptosis/death or intracellular Ca2+ at 30 mW/cm^2, though cell-cycle distribution shifted. At 100 mW/cm^2, intracellular…

[A preliminary study on role of acid sphingomyelinase in receptor clustering induced by 50-Hz magnetic fields].

Research RF Safe Research Library Jan 1, 2009

This in vitro study exposed human amnion (FL) cells to a 50-Hz sinusoidal magnetic field (0.4 mT) for 15 minutes and assessed epidermal growth factor receptor (EGFR) clustering. The authors report that magnetic field exposure induced EGFR clustering, and that this effect was blocked by imipramine, an acid…

Velocity modulation of microtubules in electric fields.

Research RF Safe Research Library Jan 1, 2008

This in vitro study reports that electric fields can control the velocity of microtubules gliding over a kinesin-coated surface. Assisting fields increased speed up to fivefold, while opposing fields slowed motion to zero, and sideways fields induced significant motion. The authors present a grab-and-release model to…

[Effect of 1.8 GHz radiofrequency electromagnetic fields on gene expression of rat neurons].

Research RF Safe Research Library Jan 1, 2008

This in vitro study exposed primary cultured rat neurons to 1.8 GHz RF-EMF at an average SAR of 2 W/kg and assessed gene expression changes. The authors report multiple differentially expressed genes after 24 h intermittent exposure and confirm exposure-dependent changes in Egr-1, Mbp, and Plp by semi-quantitative…

Use of terahertz electromagnetic radiation for correction of blood rheology parameters in patients with unstable angina under conditions of treatment with isoket, an NO donor.

Research RF Safe Research Library Jan 1, 2008

This in vitro study examined terahertz electromagnetic radiation at NO-related spectral frequencies and its effects on blood rheology in patients with unstable angina treated with the NO donor isoket. The abstract reports that irradiated isoket had a better normalizing effect on blood viscosity and erythrocyte…

Noninvasive radiofrequency field-induced hyperthermic cytotoxicity in human cancer cells using cetuximab-targeted gold nanoparticles.

Research RF Safe Research Library Jan 1, 2008

This in vitro study reports that shortwave (MHz-range) RF exposure can induce strong cytotoxicity in EGFR-overexpressing human cancer cells when those cells have internalized cetuximab-targeted gold nanoparticles. Control nanoparticles showed minimal uptake and substantially lower cytotoxicity under RF exposure. An…

Intestinal MMC-related electric fields and pancreatic juice control the adhesion of Gram-positive and Gram-negative bacteria to the gut epithelium--in vitro study.

Research RF Safe Research Library Jan 1, 2008

This in vitro study tested whether a weak electric field reconstructed from duodenal spiking potentials during an MMC cycle affects bacterial adhesion to Caco-2 intestinal epithelial cells. The MMC-related electric field increased adhesion of Lactobacillus and Lactococcus strains, while two pathogenic strains…

In vivo quantification of contrast agent concentration using the induced magnetic field for time-resolved arterial input function measurement with MRI.

Research RF Safe Research Library Jan 1, 2008

This Medical Physics article presents a phase-based MRI method to quantify paramagnetic contrast agent concentration by inverting susceptibility-induced magnetic field shifts, aiming to improve arterial input function (AIF) measurement in DCE-MRI. After in vitro validation, the approach was applied in vivo in 14…

Proteomic analysis in human fibroblasts by continuous exposure to extremely low-frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2007

This in vitro study examined whether continuous extremely low-frequency electromagnetic fields (3 Hz, 4 mT for 3 hours) alter protein expression in human fibroblast cells using proteomics. The authors report that some protein expressions differed between exposed and sham cells. They identify reduced expression (about…

Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field.

Research RF Safe Research Library Jan 1, 2007

This in vitro study measured biophoton/chemiluminescence emission from MDCK cells after inducing reactive oxygen species with hydrogen peroxide. The authors report that photon emission increased with hydrogen peroxide dose and that photomultiplier tube measurements agreed with fluorescence microscopy observations.…

[Fluorescence spectra analysis of papain treated by pulsed electric field].

Research RF Safe Research Library Jan 1, 2007

This in vitro study used fluorescence excitation, emission, and polarization spectra to assess structural changes in papain after pulsed electric field (PEF) treatment. Under the reported PEF conditions (50 kV/cm, 1500 Hz, 40 µs pulses, 19,800 pulses), papain activity decreased by 56.5%. Spectral changes (increased…

Electric field-induced astrocyte alignment directs neurite outgrowth.

Research RF Safe Research Library Jan 1, 2006

This in vitro study examined whether astrocyte alignment induced by an applied electric field influences neurite outgrowth. Astrocytes were pre-aligned using a physiological-strength electric field (500 mV mm(-1)), and dorsal root ganglia cells were then seeded onto them. Neurite outgrowth at 24 and 48 hours was…

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