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Filters: tag: Protein Expression Clear

Proteomic Characterization of Human Peripheral Blood Mononuclear Cells Exposed to a 50 Hz Magnetic Field

Research RF Safe Research Library Jan 1, 2025

This in vitro study compared proteomic profiles of PBMCs from three human donors after 24-hour exposure to a 50 Hz, 1 mT extremely low-frequency magnetic field versus unexposed cells. The abstract reports broad protein expression changes, including upregulation of proteins associated with metabolic processes and…

Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy.

Research RF Safe Research Library Jan 1, 2017

This study reports a technology that records a specific radiofrequency electromagnetic energy signal associated with EGFR siRNA and applies it to cells. The abstract describes reductions in EGFR mRNA and protein expression versus controls, with specificity assessed using RNA microarray and antibody dot blot array. It…

[Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation].

Research RF Safe Research Library Jan 1, 2016

This in vitro study exposed rat calvarial osteoblasts to 50 Hz, 0.6 mT pulsed electromagnetic fields for 1.5 hours and measured early differentiation-related markers over 0–12 hours. The abstract reports increased ALP activity at 3 hours and time-dependent changes in ALP, Runx2, and OSX transcription and Runx2/OSX…

Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

Research RF Safe Research Library Jan 1, 2014

This in vitro study exposed SH-SY5Y human neuroblastoma cells to a 2 mT, 50 Hz magnetic field for 3 hours and assessed protein expression using proteomics. The authors report that 189 proteins changed expression in exposed cells compared with controls, with PCA/clustering separating samples by condition. They…

Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2013

This in vitro study examined whether extremely low frequency electromagnetic field (ELF-EMF) exposure alters MMP-2 expression in cultured human fetal scleral fibroblasts and whether puerarin modifies this response. The authors report increased MMP-2 mRNA and protein expression after 0.2 mT ELF-EMF exposure for 24…

Low-frequency electromagnetic fields do not alter responses of inflammatory genes and proteins in human monocytes and immune cell lines.

Research RF Safe Research Library Jan 1, 2012

This in vitro study examined whether low-frequency electromagnetic fields (a complex multiple waveform field and a 50 Hz sine wave) alter inflammatory gene and protein responses in human immune cells. Across time course experiments in THP-1 cells and primary monocytes/macrophages, the authors report no significant…

Down-regulation of metallothionein 1 and 2 after exposure to electromagnetic field in mouse testis.

Research RF Safe Research Library Jan 1, 2011

This animal study examined mouse testis after 8 weeks of exposure to a 3 MT electromagnetic field (4 hours/day). The authors report significant histological changes (increased primary spermatocytes, increased Leydig cells, and increased basement membrane thickness) in exposed mice versus controls. MT1 and MT2…

[A aquaporin 4 expression and effects in rat hippocampus after microwave radiation].

Research RF Safe Research Library Jan 1, 2009

This rat study examined hippocampal aquaporin 4 (AQP4) expression after microwave exposure at 10, 30, or 100 mW/cm^2. The authors report increased AQP4 protein and gene expression after exposure, with recovery at lower power density but more persistent elevation at higher power density. They conclude that increased…

Proteomic analysis in human fibroblasts by continuous exposure to extremely low-frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2007

This in vitro study examined whether continuous extremely low-frequency electromagnetic fields (3 Hz, 4 mT for 3 hours) alter protein expression in human fibroblast cells using proteomics. The authors report that some protein expressions differed between exposed and sham cells. They identify reduced expression (about…

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