This in vitro study compared proteomic profiles of PBMCs from three human donors after 24-hour exposure to a 50 Hz, 1 mT extremely low-frequency magnetic field versus unexposed cells. The abstract reports broad protein expression changes, including upregulation of proteins associated with metabolic processes and downregulation of proteins linked to T cell costimulation/activation and immune processes. No effects were observed on cell proliferation, viability, or cell cycle progression. The authors interpret the proteomic shifts as metabolic reprogramming with potential implications for immune regulation.

This animal study examined continual 2.4 GHz Wi‑Fi exposure (200–500 μW/m²) during 9 days of chicken embryo incubation and assessed the mesonephros at day 9. The authors report no adverse effects on general mesonephros development, but describe moderate degenerative changes and vascular congestion without inflammatory infiltrate. They also report significantly increased apoptotic and proliferating cells and up-regulation of caspase‑1 gene expression, interpreted as disruption of regulatory processes during development.

This in vitro study exposed HT-1080 human fibrosarcoma cells for 4 days to weak extremely low frequency magnetic fields (10 μT, 12–33 Hz) superimposed on a 45 μT static field. The authors report frequency- and amplitude-dependent increases or decreases in cell growth, including sharp inversions near 16.5 Hz with small parameter changes or reversal of the static field direction. Associated changes in membrane potential, intracellular calcium, and mitochondrial superoxide are presented as supporting a bioenergetic mechanism.

This in vitro study exposed glioblastoma (CT2A), neuroblastoma (N2A), and non-tumor astrocyte (C8D1A) cell models to a 100 μT magnetic field across 20–100 Hz for 24–72 hours. The abstract reports decreased viability and proliferation in the tumor cell models within a frequency window centered at 50 Hz, while astrocyte viability increased at 20 and 40 Hz. The authors conclude that frequency is a key determinant of cell-type-specific responses consistent with a “biological window” model.

This review summarizes reported biological effects of extremely low frequency (ELF) electric and magnetic fields, describing them as significant and often acting as stressors. Reported outcomes include metabolic, hormonal, and body weight changes in rodents, lethality at high exposure levels in mice and insects, and increased mitotic index in mouse tissues/cells under specified exposure conditions. The review suggests many effects may be mediated through neuroendocrine, nervous system, or behavioral responses to field exposure.