Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro.
Research
RF Safe Research Library
Jan 1, 2023
This in vitro study tested whether ELF-EMF exposure (50 Hz, 1 mT; 30 min/day) influences osteogenic differentiation markers in rat bone-marrow-derived stem cells cultured in osteogenic medium or in human adipose-stem cell-conditioned medium. The OM + EMF condition showed higher ALP activity and higher Runx2 and Ocn…
Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study examined intermittent extremely low-frequency electromagnetic field (ELF-EMF) exposure (6 h/day) in human periodontal ligament mesenchymal stem cells during osteogenic differentiation. After 10 days, ELF-EMF-exposed cells showed increased proliferation and calcium deposition versus sham.…
Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage.
Research
RF Safe Research Library
Jan 1, 2022
This in vitro study exposed human adipose-derived mesenchymal stem progenitor cells to an alternating EMF generated by the IteraCoil device at 0.05 and 1 kHz. The authors report that EMF exposure was associated with osteoblastic differentiation in the absence of exogenous pro-osteogenic supplements, assessed by…
Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…
Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro and rat-model study evaluated sinusoidal low-frequency EMF combined with a PCL/nHA scaffold seeded with BMSCs for intervertebral fusion. The authors report enhanced osteogenic differentiation of BMSCs and pro-osteogenic effects of conditioned medium from EMF-treated BMSCs, with suggested involvement of…
Effects of electromagnetic fields treatment on rat critical-sized calvarial defects with a 3D-printed composite scaffold.
Research
RF Safe Research Library
Jan 1, 2020
This animal and in vitro study evaluated electromagnetic fields (EMF) treatment combined with a 3D-printed PLA/HA scaffold seeded with rat bone marrow mesenchymal stem cells (BMSCs) for cranial defect repair. The authors report that EMF enhanced BMSC proliferation and osteogenic differentiation and was partly…
Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction.
Research
RF Safe Research Library
Jan 1, 2019
This study examined how electromagnetic field (EMF) treatment influences osteogenic differentiation mechanisms in human bone marrow mesenchymal stem cells and tested a combined therapy in an ovariectomized rat osteoporosis model. The authors report that EMF exposure increased P2X7 receptor expression during…
Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study examined rat adipose-derived mesenchymal stem cells exposed to zinc sulphate in the presence of an ELF electromagnetic field (50 Hz, 20 mT). The authors report increased osteogenic differentiation markers (gene expression, ALP activity, and calcium levels) under combined ZnSO4+EMF conditions,…