Stable morphological-physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study evaluated combined effects of extremely low frequency EMF (50 Hz, 20 mT) and nitric oxide (NO) on rat bone marrow mesenchymal stem cells. The authors report reduced viability and morphology changes with high NO in the presence of EMF, along with increased calcium ion entry. They also report…
Different profiles of the mRNA levels of DNA repair genes in MCF-7 and SH-SY5Y cells after treatment with combination of cisplatin, 50-Hz electromagnetic field and bleomycin.
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RF Safe Research Library
Jan 1, 2017
This in vitro study examined whether a 50-Hz, 0.50 mT intermittent electromagnetic field modifies responses to cisplatin and bleomycin in MCF-7 and SH-SY5Y cells. The authors report increased susceptibility in MCF-7 cells with EMF added to cisplatin+bleomycin, while SH-SY5Y susceptibility was unchanged. They also…
Effects of radiofrequency exposure emitted from a GSM mobile phone on proliferation, differentiation, and apoptosis of neural stem cells.
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RF Safe Research Library
Jan 1, 2017
This in vitro study evaluated adult murine neural stem cells exposed to GSM 900-MHz radiofrequency emissions from a mobile phone across different exposure durations. The authors report significant decreases in neurosphere number/size and neuronal differentiation with increasing exposure duration. They report no…
Changes in viability of rat adipose-derived stem cells isolated from abdominal/perinuclear adipose tissue stimulated with pulsed electromagnetic field.
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RF Safe Research Library
Jan 1, 2017
This in vitro study exposed rat adipose-derived stem cells to a pulsed electromagnetic field (7 Hz, 30 mT) and assessed viability by flow cytometry. The authors report that a low-fat diet was protective in PEMF-exposed ADSCs, especially in pups. They also report a higher proportion of early apoptotic cells in…
Cytotoxicity of temozolomide on human glioblastoma cells is enhanced by the concomitant exposure to an extremely low-frequency electromagnetic field (100Hz, 100G).
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RF Safe Research Library
Jan 1, 2017
This in vitro study tested whether an extremely low-frequency EMF (100 Hz, 100 G) could enhance temozolomide cytotoxicity in human glioma cell lines (U87 and T98G). The authors report that combined EMF and temozolomide increased apoptosis relative to either treatment alone and was accompanied by changes in…
Vitamin C and Vitamin E Protected B and Balb/c-3T3 Cells from Apoptosis Induced by Intermittent 50Hz ELF-EMF Radiation.
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RF Safe Research Library
Jan 1, 2017
This in vitro study exposed B cells and Balb/c-3T3 cells to intermittent 50 Hz power-line ELF-EMF and assessed apoptosis. The authors report that preincubation with vitamin C or vitamin E reduced apoptosis compared with the exposed group. They conclude that vitamins C and E have protective effects, with stronger…
Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways.
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RF Safe Research Library
Jan 1, 2017
This in vitro study examined rat adipose-derived mesenchymal stem cells exposed to zinc sulphate in the presence of an ELF electromagnetic field (50 Hz, 20 mT). The authors report increased osteogenic differentiation markers (gene expression, ALP activity, and calcium levels) under combined ZnSO4+EMF conditions,…
Semi-quantitative proteomics of mammalian cells upon short-term exposure to non-ionizing electromagnetic fields.
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RF Safe Research Library
Jan 1, 2017
This in vitro study used proteome-wide semi-quantitative mass spectrometry to assess molecular changes after short-term exposure to ELF (50 Hz), UMTS (2.1 GHz), and WiFi (5.8 GHz) in human and mouse cell types. The authors report that fewer than 1% of quantified proteins changed slightly in abundance and that…
Evaluation of the Effect of Radiofrequency Radiation Emitted From Wi-Fi Router and Mobile Phone Simulator on the Antibacterial Susceptibility of Pathogenic Bacteria and .
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RF Safe Research Library
Jan 1, 2017
This in vitro study assessed whether RF-EMF exposure from a GSM 900 MHz mobile phone simulator and a 2.4 GHz Wi-Fi router alters bacterial susceptibility to antibiotics. The authors report significant changes in inhibition zone diameters and bacterial growth rate after exposure. They also describe an exposure window…
Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization.
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RF Safe Research Library
Jan 1, 2017
This in vitro study exposed human microvascular endothelial cells to high-power microwave radiation at multiple power densities for 6 minutes and assessed cellular injury and cytoskeletal outcomes. The authors report dose-dependent injury from microwave exposure, including increased LDH leakage and reduced viability…
MICROWAVE RADIATION INDUCED SYNTHESIS OF HYDROXYPROPYL METHYLCELLULOSE-GRAFT- (POLYVINYLALCOHAL-CO-ACRYLIC ACID) POLYMERIC NETWORK AND ITS IN VITRO EVALUATION.
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RF Safe Research Library
Jan 1, 2017
This in vitro materials study compares microwave irradiation versus conventional water-bath heating for synthesizing HPMC-graft-(PVA-co-acrylic acid) hydrogels loaded with captopril. The authors report that microwave-synthesized hydrogels had more uniformly porous structures and higher swelling ratios, with…
Disinfection effect of microwave radiation on Bacillus subtilis as indicator organism on contaminated dental stone casts under dry and wet conditions.
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RF Safe Research Library
Jan 1, 2017
This in vitro study evaluated microwave disinfection of dental stone casts contaminated with Bacillus subtilis under wet and dry conditions. After a drying step for the dry group (450 W for 15 minutes), both wet and dry casts were exposed to 900 W for 5 minutes and cultured to quantify CFU/mL. The authors report…
A comparison of the efficacy of mechanical, chemical, and microwave radiation methods in disinfecting complete dentures.
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RF Safe Research Library
Jan 1, 2017
This in vitro study compared microwave irradiation with chemical (2% glutaraldehyde, Corega tablets) and mechanical (brushing) methods for disinfecting complete dentures contaminated with two microorganisms. Microwave irradiation (650 W for 3 minutes) and both chemical methods showed no detectable bacterial growth at…
Microwave radiation is effective at disinfecting dental stone surfaces without changing their physical properties.
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study tested 900-W microwave radiation regimens (3, 5, or 7 minutes) to disinfect type IV dental stone surfaces contaminated with S. aureus, E. coli, or C. albicans. The authors report that 5 and 7 minutes achieved sterilization for S. aureus, while 3 minutes did not fully sterilize; all times were…
[Protective effects of Genistein on human renal tubular epithelial cells damage of microwave radiation].
Research
RF Safe Research Library
Jan 1, 2017
This in vitro study exposed human proximal renal tubular epithelial cells (HKC) to microwave irradiation for 24 hours and assessed cell injury, oxidative stress, and cell death. The authors report increased injury markers (LDH, NAG), increased oxidative stress (higher MDA, lower SOD), and more apoptosis/necrosis…
Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field.
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RF Safe Research Library
Jan 1, 2016
This in vitro study describes a method to improve DNA ligation by immobilizing T4 DNA ligase on ferromagnetic particles and applying a radio frequency alternating magnetic field. The field causes the particles to dissipate heat, which the authors report selectively heats and activates the ligase while minimally…
Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field.
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RF Safe Research Library
Jan 1, 2016
This paper describes an in vitro gene transfection technique termed water-in-oil droplet electroporation, using an intense DC electric field to deform a droplet between electrodes. The deformation can cause an instantaneous short circuit, during which transient membrane pores are reported to form and permit exogenous…
Targeted Magnetic Nanoparticles for Mechanical Lysis of Tumor Cells by Low-Amplitude Alternating Magnetic Field.
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RF Safe Research Library
Jan 1, 2016
This animal and in vitro study reports a method to lyse tumor cells mechanically using targeted superparamagnetic iron oxide nanoparticles rotated by a weak alternating magnetic field. Nude mice with head and neck tumors received cetuximab-coated nanoparticles and 4 Hz field exposures for 30 minutes on alternating…
Research on in vitro release of Isoniazid (INH) super paramagnetic microspheres in different magnetic fields.
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RF Safe Research Library
Jan 1, 2016
This in vitro study examined how different external magnetic field conditions affect isoniazid release from superparamagnetic iron oxide microspheres. The abstract reports that magnetic fields accelerated drug release, with faster release in acidic (pH 3) conditions and the fastest completion (2 hours) under an…
[The influence of alternating magnetic field on in vitro litholysis of uroliths in blemaren water solutions].
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RF Safe Research Library
Jan 1, 2016
This in vitro study examined whether exposing Blemaren water solutions (pH 6.15) to a 2 Hz alternating magnetic field for one hour changes their ability to dissolve uroliths. The authors report higher dissolution efficiency (about 1.922d7) in pre-exposed solutions compared with non-exposed controls. They also report…
[Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation].
Research
RF Safe Research Library
Jan 1, 2016
This in vitro study exposed rat calvarial osteoblasts to 50 Hz, 0.6 mT pulsed electromagnetic fields for 1.5 hours and measured early differentiation-related markers over 0–12 hours. The abstract reports increased ALP activity at 3 hours and time-dependent changes in ALP, Runx2, and OSX transcription and Runx2/OSX…
Effect of long-term exposure to mobile phone radiation on alpha-Int1 gene sequence of Candida albicans.
Research
RF Safe Research Library
Jan 1, 2016
This in vitro study evaluated whether long-term 900 MHz radiation exposure induces mutations in the alpha-Int1 gene of Candida albicans. Yeast samples were exposed for 10 to 490 hours at 4°C and compared with unexposed controls. Sequencing of the PCR-amplified alpha-Int1 fragment found no mutations in exposed samples…
Unraveling the effects of static magnetic field stress on cytosolic proteins of Salmonella by using a proteomic approach.
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RF Safe Research Library
Jan 1, 2016
This in vitro study examined how Salmonella enterica serovar Hadar adapts to a 200 mT static magnetic field for 9 hours using proteomics. The authors report 35 cytosolic proteins with >2-fold expression changes after exposure, spanning metabolism, oxidative stress response, energy homeostasis, translation elongation,…
Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway
Research
RF Safe Research Library
Jan 1, 2015
This in vitro/ex vivo study examined whether low-frequency electromagnetic field exposure affects human neutrophil extracellular trap (NET) formation. The authors report that LF EMF exposure enhanced NET formation and that this effect depended on the NADPH oxidase pathway and reactive oxygen species production. They…
[Synergistic inhibitory effect of static magnetic field and antitumor drugs on Hepa1-6 cells].
Research
RF Safe Research Library
Jan 1, 2015
This in vitro study tested whether a static magnetic field (SMF) could enhance the effects of cisplatin or adriamycin in Hepa1-6 tumor cells. The abstract reports that SMF combined with either drug increased growth inhibition and was associated with cell-cycle arrest, increased DNA damage, and more severe membrane…