Effects induced by a 50 Hz electromagnetic field and doxorubicin on Walker-256 carcinosarcoma growth and hepatic redox state in rats.
Research
RF Safe Research Library
Jan 1, 2021
This animal study compared 50 Hz extremely low-frequency EMF exposure with doxorubicin (and their combination) in Walker-256 carcinosarcoma-bearing rats, assessing tumor growth and liver redox-related outcomes. DOX and DOX+EMF were reported to inhibit tumor growth more than EMF alone, though EMF alone also showed an…
Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…
Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…
Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro and rat-model study evaluated sinusoidal low-frequency EMF combined with a PCL/nHA scaffold seeded with BMSCs for intervertebral fusion. The authors report enhanced osteogenic differentiation of BMSCs and pro-osteogenic effects of conditioned medium from EMF-treated BMSCs, with suggested involvement of…
Low-frequency electromagnetic field influences human oral mucosa keratinocyte viability in response to lipopolysaccharide or minocycline treatment in cell culture conditions.
Research
RF Safe Research Library
Jan 1, 2021
This in vitro study examined whether low-frequency electromagnetic field (LF-EMF) exposure affects viability of human oral mucosa keratinocytes. Apoptosis (early/late) and necrosis were measured by flow cytometry, including conditions with lipopolysaccharide (LPS) or minocycline. The authors report that LF-EMF, alone…
Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.
Research
RF Safe Research Library
Jan 1, 2020
This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…
Electromagnetic field affects the voltage-dependent potassium channel Kv1.3.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study tested whether extremely low-frequency electromagnetic fields (ELF-EMF) modulate the voltage-gated potassium channel Kv1.3 in Kv1.3-expressing CHO-K1 cells. Cells were exposed to a 20 Hz field at 268 µT or 902 µT while Kv1.3 currents were measured by whole-cell patch clamp. The authors report a…
Low-frequency electromagnetic fields promote hair follicles regeneration by injection a mixture of epidermal stem cells and dermal papilla cells.
Research
RF Safe Research Library
Jan 1, 2020
This animal study evaluated whether low-frequency EMF exposure affects bioengineered hair follicle generation in nude mice injected with a mixture of epidermal stem cells and dermal papilla cells. Histology (H&E) at 7 or 14 days suggested more hair follicle eruption and higher density of hair follicle-like structures…
Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study used spectroscopic and conductometry methods to examine binding between human hemoglobin and an angiotensin-converting enzyme inhibitory peptide, with and without electromagnetic field (EMF) exposure. The authors report that EMF changed the Hb–ACEIP interaction and that binding was associated with…
Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats.
Research
RF Safe Research Library
Jan 1, 2020
This rat study examined chronic extremely low-frequency electromagnetic field (ELF-EMF) exposure and diaphragm muscle function and structure. Rats were exposed to 50 Hz, 1.5 mT for 4 hours daily for up to 7 months and assessed with electrophysiological, biochemical, and electron-microscopy measures. The authors…
The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…
Exploring the form- And time-dependent effect of low-frequency electromagnetic fields on maintenance of hippocampal long-term potentiation.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study developed an MEA-compatible magnetic stimulation device to deliver low-frequency EMFs during hippocampal LTP experiments without interrupting induction. The authors report that sine-wave LF-EMFs applied post-induction or as priming reduced fEPSP slope during LTP maintenance, with stronger effects…
Immune response of mollusk Onchidium struma to extremely low-frequency electromagnetic fields (ELF-EMF, 50 Hz) exposure based on immune-related enzyme activity and De novo transcriptome analysis.
Research
RF Safe Research Library
Jan 1, 2020
This animal study exposed the mollusk Onchidium struma to 50 Hz ELF-EMF at 100 μT or 500 μT for up to one week and assessed immune-related cell counts, enzyme activities, and transcriptomic changes. The 100 μT group showed increased total coelomocyte and spherulocyte density versus control and 500 μT, while some cell…
Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.
Research
RF Safe Research Library
Jan 1, 2020
This in vitro study exposed human dermal papilla cells to 70 Hz extremely low-frequency electromagnetic fields at 5–100 G for four days. The abstract reports increased expression of several anagen-related molecules and increased proliferation, with 10 G described as the most potent condition. It also reports…
Lipidomic alteration and stress-defense mechanism of soil nematode Caenorhabditis elegans in response to extremely low-frequency electromagnetic field exposure.
Research
RF Safe Research Library
Jan 1, 2019
This animal (nematode) study exposed Caenorhabditis elegans to 50 Hz, 3 mT extremely low-frequency EMF and assessed multi-omics responses. The authors report significant lipidomic, proteomic, and transcriptomic alterations, including increased triacylglycerols. Pathway analyses suggest disrupted lipid metabolism,…
T-Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low-Frequency Electromagnetic Fields Via Induction of IL-2 Signaling.
Research
RF Safe Research Library
Jan 1, 2019
This in vitro study examined 60 Hz extremely low-frequency EMF exposure (0.8 mT) on CD4 T cells under Th9-polarizing conditions. The authors report increased Th9-associated responses including IL-9 secretion, transcription factor changes (including early GATA3), and increased STAT5/STAT6 phosphorylation alongside…
Low-frequency electromagnetic fields as an alternative to sanitize water of drinking systems in poultry production?
Research
RF Safe Research Library
Jan 1, 2019
This study tested pulsed low-frequency electromagnetic fields (up to 10,000 Hz; max 21 mT) as a potential alternative sanitation approach for poultry drinking water systems. LF-EMF exposure was linked to variable changes in culturable bacterial counts, with direction depending on system type and some early…
Impact of extremely low-frequency electromagnetic field (100 Hz, 100 G) exposure on human glioblastoma U87 cells during Temozolomide administration.
Research
RF Safe Research Library
Jan 1, 2019
This in vitro study evaluated whether extremely low-frequency EMF exposure (100 Hz, 100 G) modifies the effects of temozolomide (100 µM) in human glioblastoma U87 cells over 120–144 hours. The authors report that combined treatment reduced stemness-associated markers (Nestin, CD133, Notch4) and increased GFAP…
Correlation between the Lunar Phase and Tail-Lifting Behavior of Lizards () Exposed to an Extremely Low-Frequency Electromagnetic Field.
Research
RF Safe Research Library
Jan 1, 2019
This animal study examined long-term tail-lifting behavior in agamid lizards exposed to an ELF electromagnetic field (6 and 8 Hz; peak magnetic field 2.6 µT; peak electric field 10 V/m) versus controls, incorporating lunar phase and geomagnetic activity (K index) as explanatory variables. Regression analyses found…
Geobacter sulfurreducens-inoculated bioelectrochemical system reveals the potential of metabolic current in defining the effect of extremely low-frequency electromagnetic field on living cells.
Research
RF Safe Research Library
Jan 1, 2019
This laboratory study exposed a Geobacter sulfurreducens-inoculated bioelectrochemical system to 0–128 Gauss extremely low-frequency electromagnetic fields. The authors report increased and oscillatory metabolic current under ELF-EMF exposure, with dose-dependent effects and a linear relationship between oscillation…
Effects of single and combined low frequency electromagnetic fields and simulated microgravity on gene expression of human mesenchymal stem cells during chondrogenesis.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study exposed human mesenchymal stem cell pellets undergoing chondrogenesis to simulated microgravity (SMG), low-frequency EMF (5 mT, 15 Hz), or both for 3 weeks. SMG significantly changed gene expression and reduced chondrogenic marker expression (including COL2A1). LF-EMF alone showed no…
Acute exposure to high-induction electromagnetic field affects activity of model peripheral sensory neurons.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study examined acute low-frequency electromagnetic field (LF-EMF) exposure using high-induction magnetic stimulation in F11 cells, a dorsal root ganglia neuron-derived model of peripheral sensory neurons. Short-term exposure (
Assessment of an anti-scale low-frequency electromagnetic field device on drinking water biofilms.
Research
RF Safe Research Library
Jan 1, 2018
This laboratory study tested a low-intensity very low-frequency EMF anti-scale device for potential impacts on drinking water biofilms in flow-through reactors. Across soft and hard water and for nascent and mature biofilms, the EMF exposure did not significantly change biofilm cell numbers, cell integrity measures,…
Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis.
Research
RF Safe Research Library
Jan 1, 2018
This in vitro study used a 3D dermal papilla-like tissue model containing dermal papilla cells and melanocytes to assess melanogenesis after rice bran extract (RBE), extremely low-frequency EMF, or their combination. The combined RBE/EMF condition showed higher tyrosinase activity, increased melanogenesis-related…