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Changes in Free Radical Processes under the Influence of Low-Frequency Electromagnetic Field in Rats.

Research RF Safe Research Library Jan 1, 2022

This in vivo animal study examined how low-frequency electromagnetic field exposure affects antioxidant status in Wistar rats. The authors report frequency-dependent changes in antioxidant enzyme activity and hydroperoxide content in blood plasma. They also propose that LF EMF may stimulate low levels of ROS that…

Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…

The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…

Necroptosis triggered by ROS accumulation and Ca overload, partly explains the inflammatory responses and anti-cancer effects associated with 1Hz, 100 mT ELF-MF in vivo.

Research RF Safe Research Library Jan 1, 2021

This animal and in vitro study examined ELF magnetic field exposure in a mouse breast tumor model and a breast cancer cell line. The authors report tumor growth suppression and reduced expression of Ki-67, CD31, VEGFR2, and MMP-9 after exposure. They also report increased IFN-γ and TNF-α and increased…

Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed HaCaT human keratinocytes to 1760 MHz RF-EMF (SAR 4.0 W/kg; 2 h/day for 4 days). The authors report increased intracellular ROS and increased expression/activity of several matrix metalloproteinases, along with increased phosphorylation of FoxO3a and ERK1/2. Cell growth/viability and…

Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.

Research RF Safe Research Library Jan 1, 2020

This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…

Impact of electromagnetic fields on in vitro toxicity of silver and graphene nanoparticles.

Research RF Safe Research Library Jan 1, 2019

This in vitro study examined whether RF electromagnetic fields (900, 2400, 7500 MHz) modify the toxicity of silver and graphene nanoparticles in human fibroblast cultures. The authors report EMF-associated changes in cytotoxicity and ROS that depended on nanoparticle type, shape, and concentration, with the strongest…

A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels.

Research RF Safe Research Library Jan 1, 2018

This in vitro study examined effects of a 6 mT, 60 Hz uniform ELF electromagnetic field on HeLa cells and primary IMR-90 fibroblasts. Single or repetitive exposure did not induce DNA damage or change viability, but continuous exposure over 168 hours was reported to increase proliferation in both cell types in a…

Effects of Fifty-Hertz Electromagnetic Fields on Granulocytic Differentiation of ATRA-Treated Acute Promyelocytic Leukemia NB4 Cells.

Research RF Safe Research Library Jan 1, 2018

This in vitro study examined whether 50 Hz, 2 mT extremely low-frequency electromagnetic fields affect ATRA-driven granulocytic differentiation in NB4 acute promyelocytic leukemia cells. The authors report reduced proliferation and enhanced maturation/differentiation markers with ELF-EMF exposure. They also report…

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells.

Research RF Safe Research Library Jan 1, 2017

This in vitro study compared ginsenosides extracted using pulsed electric field (PEF) versus solvent cold soak extraction (SCSE). The abstract reports that PEF extracts had higher antioxidant capacity and provided greater protection against hydrogen peroxide-induced oxidative stress in HEK-293 cells, including…

Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544.

Research RF Safe Research Library Jan 1, 2017

This in vitro study exposed human keratinocyte cells (NCTC 2544) to 50 Hz ELF-EMF at 0.25–2 G and assessed oxidative stress-related endpoints. Significant increases in ROS were reported at 0.5 and 1 G, with associated short-term changes in antioxidant and lipid peroxidation markers. The authors report that…

Exposure to 2100 MHz electromagnetic field radiations induces reactive oxygen species generation in roots.

Research RF Safe Research Library Jan 1, 2017

This experimental study examined whether 2100 MHz cell phone-related RF-EMF exposure affects oxidative stress markers in onion roots. After 1–4 hours of continuous-wave exposure in a single day, the study reports increased lipid peroxidation and ROS-related measures (MDA, hydrogen peroxide, superoxide anion).…

Cytotoxicity of temozolomide on human glioblastoma cells is enhanced by the concomitant exposure to an extremely low-frequency electromagnetic field (100Hz, 100G).

Research RF Safe Research Library Jan 1, 2017

This in vitro study tested whether an extremely low-frequency EMF (100 Hz, 100 G) could enhance temozolomide cytotoxicity in human glioma cell lines (U87 and T98G). The authors report that combined EMF and temozolomide increased apoptosis relative to either treatment alone and was accompanied by changes in…

Low-Frequency Electromagnetic Field Exposure Enhances Extracellular Trap Formation by Human Neutrophils through the NADPH Pathway

Research RF Safe Research Library Jan 1, 2015

This in vitro/ex vivo study examined whether low-frequency electromagnetic field exposure affects human neutrophil extracellular trap (NET) formation. The authors report that LF EMF exposure enhanced NET formation and that this effect depended on the NADPH oxidase pathway and reactive oxygen species production. They…

The effect of electromagnetic radiation emitted by display screens on cell oxygen metabolism - in vitro studies.

Research RF Safe Research Library Jan 1, 2015

This in vitro study exposed human blood platelet suspensions to 1 kHz electromagnetic fields at 150 or 220 V/m for 30 or 60 minutes. The authors report increased ROS and MDA (at 220 V/m, 30 min) and decreased antioxidant enzyme activities (SOD-1 and catalase), with the largest changes at 220 V/m for 60 minutes. They…

Assessment of the neurotoxic potential of exposure to 50Hz extremely low frequency electromagnetic fields (ELF-EMF) in naïve and chemically stressed PC12 cells

Research RF Safe Research Library Jan 1, 2014

This in vitro study tested acute (30 min) and sub-chronic (48 h) 50 Hz ELF-EMF exposure up to 1000 µT in naïve and chemically stressed PC12 cells. Across assays of Ca2+ homeostasis, ROS production, and membrane integrity, the study reports no consistent or measurable neurotoxic effects under the tested conditions.…

Reactive oxygen species elevation and recovery in Drosophila bodies and ovaries following short-term and long-term exposure to DECT base EMF.

Research RF Safe Research Library Jan 1, 2014

This animal study measured ROS in Drosophila bodies and ovaries after short- and long-term exposure to DECT base RF (1.88–1.90 GHz; SAR 0.009 W/kg). The abstract reports increased ROS in bodies after 6 h exposure and in ovaries after 0.5–1 h exposure, with apparent saturation under longer continuous exposure. It also…

Nanosecond pulse electric field activation of platelet-rich plasma reduces myocardial infarct size and improves left ventricular mechanical function in the rabbit heart.

Research RF Safe Research Library Jan 1, 2012

This animal and in vitro study evaluated nanosecond pulsed electric fields (nsPEF) as a nonchemical method to activate platelet-rich plasma (PRP) for cardioprotection. In rabbits undergoing brief coronary occlusion followed by reperfusion, PRP injection into ischemic myocardium was associated with reduced infarct…

900 MHz radiofrequency-induced histopathologic changes and oxidative stress in rat endometrium: protection by vitamins E and C.

Research RF Safe Research Library Jan 1, 2007

This rat study examined 900 MHz radiofrequency exposure (30 min/day for 30 days) and reported biochemical and histopathologic changes in endometrial tissue consistent with oxidative stress and tissue injury. The abstract reports increased NO and MDA and decreased antioxidant enzyme activities (SOD, CAT, GSH-Px) in…

Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field.

Research RF Safe Research Library Jan 1, 2007

This in vitro study measured biophoton/chemiluminescence emission from MDCK cells after inducing reactive oxygen species with hydrogen peroxide. The authors report that photon emission increased with hydrogen peroxide dose and that photomultiplier tube measurements agreed with fluorescence microscopy observations.…

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