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First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed adipose tissue-derived mesenchymal stem cells to a continuous 50 Hz electromagnetic field at 1.5 mT for 24, 48, or 72 hours and assessed micromolecular changes using FTIR spectroscopy. The authors report the largest FTIR spectral differences versus control in nucleic acids and proteins…

EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.

Research RF Safe Research Library Jan 1, 2021

This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits.

Research RF Safe Research Library Jan 1, 2021

This animal and in vitro study evaluated a composite osteochondral scaffold with bone marrow mesenchymal stem cells (BMSCs) combined with low-frequency electromagnetic field (EMF) stimulation. The authors report that EMF promoted BMSC proliferation and chondrogenic differentiation in vitro, partly via PI3K/AKT/mTOR…

The Time-Dependence of Three Different Modes of ELF-EMF Stimulation on LTP at Schaffer Collateral-CA1 Synapses.

Research RF Safe Research Library Jan 1, 2021

This animal ex vivo study tested whether short-term 15 Hz/2 mT ELF-EMF exposure alters LTP at rat hippocampal SC-CA1 synapses. Across sinusoidal, pulsed, and rhythm-pulsed modes, the authors report reduced LTP versus control, with greater inhibition at longer exposure durations (10–60 s). Sinusoidal stimulation…

The guardians of germ cells; Sertoli-derived exosomes against electromagnetic field-induced oxidative stress in mouse spermatogonial stem cells.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed mouse spermatogonial stem cells to 50 Hz EMF (2.5 mT; 1 h/day for 5 days) and assessed oxidative stress and cell health outcomes. The authors report increased ROS and apoptosis with reduced antioxidant (catalase) expression, viability, and colonization after EMF exposure. Sertoli…

Exposure to long-term evolution radiofrequency electromagnetic fields decreases neuroblastoma cell proliferation via Akt/mTOR-mediated cellular senescence.

Research RF Safe Research Library Jan 1, 2021

This in vitro study examined LTE RF-EMF exposure effects on SH-SY5Y neuroblastoma cells. The authors report reduced cell proliferation and a G0/G1 cell-cycle delay after 1760 MHz exposure at 4 W/kg for 4 hr/day over 4 days. They report no marked DNA damage signal (γH2AX) and no apoptosis activation, but increased…

Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…

Necroptosis triggered by ROS accumulation and Ca overload, partly explains the inflammatory responses and anti-cancer effects associated with 1Hz, 100 mT ELF-MF in vivo.

Research RF Safe Research Library Jan 1, 2021

This animal and in vitro study examined ELF magnetic field exposure in a mouse breast tumor model and a breast cancer cell line. The authors report tumor growth suppression and reduced expression of Ki-67, CD31, VEGFR2, and MMP-9 after exposure. They also report increased IFN-γ and TNF-α and increased…

Evaluation of the neuroprotective effects of Vitamin E on the rat substantia nigra neural cells exposed to electromagnetic field: An ultrastructural study.

Research RF Safe Research Library Jan 1, 2021

This rat study exposed animals to a 50 Hz, 3 mT electromagnetic field and assessed oxidative stress markers and substantia nigra ultrastructure. EMF exposure was associated with higher lipid peroxidation (MDA), lower SOD activity, and TEM-detected changes in nucleus/nuclear membrane and myelin sheath. Vitamin E…

Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2021

This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…

Effect of extremely low frequency electromagnetic field parameters on the proliferation of human breast cancer.

Research RF Safe Research Library Jan 1, 2021

This in vitro study tested how varying ELF-EMF parameters (frequency, flux density, duration, and continuous vs switching exposure) affect breast cancer and normal breast epithelial cell lines. The authors report that an optimized switching exposure pattern (7.83 ± 0.3 Hz, 1 mT) reduced viability of MDA-MB-231 cells…

Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia.

Research RF Safe Research Library Jan 1, 2021

This animal study evaluated whether ELF-EMF exposure affects recovery-related outcomes after cerebral ischemia in rats. Rats exposed to 50 Hz, 1 mT ELF-EMF for 2 hours daily over 28 days showed better Morris water maze performance than untreated rats. The study also reports increased hippocampal neurogenesis markers…

Synergistic effect of electromagnetic fields and nanomagnetic particles on osteogenesis through calcium channels and p-ERK signaling.

Research RF Safe Research Library Jan 1, 2021

This in vitro and animal study evaluated whether extremely low frequency EMF exposure (1 mT, 45 Hz, 8 h/day) combined with Fe-based nanomagnetic particles enhances osteogenesis. The authors report no detectable cytotoxicity and increased osteogenic markers/proteins with combined NMP + EMF exposure, alongside…

The effects of prenatal and postnatal exposure to electromagnetic field on rat ovarian tissue.

Research RF Safe Research Library Jan 1, 2020

This animal study examined prenatal-to-postnatal exposure to a 50-Hz, 3-mT electromagnetic field and ovarian development in female rat offspring. At 42 days of age, the EMF group had higher FSH, lower estradiol, higher iNOS levels/expression, and histological signs of ovarian tissue damage compared with sham. The…

Short ELF-EMF Exposure Targets SIRT1/Nrf2/HO-1 Signaling in THP-1 Cells.

Research RF Safe Research Library Jan 1, 2020

This in vitro study examined 50 Hz, 1 mT ELF-EMF exposure in LPS-treated THP-1 cells for 1–24 hours. The authors report increased HO-1 expression and Nrf2-related signaling, with nuclear enrichment of HO-1 and modulation of SIRT1/NF-κB pathways. They interpret these molecular changes as suggesting a protective,…

Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.

Research RF Safe Research Library Jan 1, 2020

This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…

Effect of Rehabilitation with Extremely Low Frequency Electromagnetic Field on Molecular Mechanism of Apoptosis in Post-Stroke Patients.

Research RF Safe Research Library Jan 1, 2020

This study evaluated whether adding extremely low frequency electromagnetic field (ELF-EMF) exposure to a rehabilitation program affects apoptosis-related gene expression in post-stroke patients. Forty-eight patients (3–4 weeks post-incident) were divided into ELF-EMF and non-ELF-EMF groups, with the ELF-EMF group…

Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats.

Research RF Safe Research Library Jan 1, 2020

This rat study examined chronic extremely low-frequency electromagnetic field (ELF-EMF) exposure and diaphragm muscle function and structure. Rats were exposed to 50 Hz, 1.5 mT for 4 hours daily for up to 7 months and assessed with electrophysiological, biochemical, and electron-microscopy measures. The authors…

The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.

Research RF Safe Research Library Jan 1, 2020

This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…

Combination of low intensity electromagnetic field with chondrogenic agent induces chondrogenesis in mesenchymal stem cells with minimal hypertrophic side effects.

Research RF Safe Research Library Jan 1, 2020

This in vitro study evaluated whether extremely low-frequency EMF (50 Hz,

Lipidomic alteration and stress-defense mechanism of soil nematode Caenorhabditis elegans in response to extremely low-frequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2019

This animal (nematode) study exposed Caenorhabditis elegans to 50 Hz, 3 mT extremely low-frequency EMF and assessed multi-omics responses. The authors report significant lipidomic, proteomic, and transcriptomic alterations, including increased triacylglycerols. Pathway analyses suggest disrupted lipid metabolism,…

The effects of the electromagnetic fields on the biochemical components, enzymatic and non-enzymatic antioxidant systems of tea L.

Research RF Safe Research Library Jan 1, 2019

This laboratory study examined how electromagnetic field (EMF) treatment affects biochemical and antioxidant-related components in tea samples, including flavonoid markers measured by HPLC. The abstract reports that 30-minute exposures generally increased several flavonoids compared with 60-minute exposures, with the…

T-Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low-Frequency Electromagnetic Fields Via Induction of IL-2 Signaling.

Research RF Safe Research Library Jan 1, 2019

This in vitro study examined 60 Hz extremely low-frequency EMF exposure (0.8 mT) on CD4 T cells under Th9-polarizing conditions. The authors report increased Th9-associated responses including IL-9 secretion, transcription factor changes (including early GATA3), and increased STAT5/STAT6 phosphorylation alongside…

Low-frequency electromagnetic fields as an alternative to sanitize water of drinking systems in poultry production?

Research RF Safe Research Library Jan 1, 2019

This study tested pulsed low-frequency electromagnetic fields (up to 10,000 Hz; max 21 mT) as a potential alternative sanitation approach for poultry drinking water systems. LF-EMF exposure was linked to variable changes in culturable bacterial counts, with direction depending on system type and some early…

APPLICATION OF TIME-AVERAGED AND INTEGRAL-BASED MEASURE FOR MEASUREMENT RESULTS VARIABILITY REDUCTION IN GSM/DCS/UMTS SYSTEMS.

Research RF Safe Research Library Jan 1, 2019

This exposure-assessment study evaluated temporal variability of RF-EMF levels from GSM/DCS/UMTS networks using continuous personal exposimeter logging. It proposes an integral-based measure (accumulated energy density from instantaneous power density) alongside 24 h time-averaging to reduce variability in daily…

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