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Time-Dependence Effect of 2.45 GHz RF-EMR Exposure on Male Reproductive Hormones and LHCGR

Research RF Safe Research Library Jan 1, 2025

This animal study exposed male Sprague Dawley rats to 2.45 GHz Wi-Fi for varying daily durations over eight weeks and assessed reproductive hormones and LHCGR expression. Serum LH and testosterone did not differ significantly from controls, but LHCGR mRNA increased with longer exposure and LHCGR protein showed decreases with shorter exposures with partial improvement at 24 hours/day. The findings suggest molecular alterations in testicular tissue despite stable systemic hormone levels.

From adults to offspring: Wi-Fi RF-EMR exposure in adult zebrafish impairs reproduction and transgenerationally effects development and behavior of progeny

Research RF Safe Research Library Jan 1, 2025

This animal study examined Wi-Fi RF-EMR exposure in adult zebrafish (4 hours/day for 30 days) and assessed reproductive tissues and offspring outcomes. The abstract reports testicular and ovarian histopathological abnormalities in exposed adults. Offspring from exposed parents, maintained under EMF-free conditions, reportedly showed increased mortality, morphological abnormalities, and anxiety-like behavior, with malformations increasing with longer parental exposure.

Modeling the interplay between myelin architecture and local electromagnetic fields

Research RF Safe Research Library Jan 1, 2025

This engineering/modeling study developed a 3D myelin microstructure model using finite element analysis and high-resolution imaging to simulate local electromagnetic field distributions. It reports that myelin architecture substantially shapes the distribution of electromagnetic fields across neural tissues. The authors suggest these field variations could potentially serve as non-invasive indicators of myelin integrity and may support tracking neurodegenerative disease progression.

Synergistic Effects of 2600 MHz Radiofrequency Exposure and Indomethacin on Oxidative Stress and Gastric Mucosal Injury in Rats

Research RF Safe Research Library Jan 1, 2025

This rat study tested whether 2600 MHz radiofrequency field exposure interacts with indomethacin to affect gastric tissue. Both exposures alone were reported to increase oxidative stress and reduce antioxidant markers in the stomach. Co-exposure was reported to intensify oxidative stress, apoptosis, and histological gastric mucosal injury compared with either factor alone, consistent with a synergistic detrimental effect in this model.

Effect of Electromagnetic Field on Oral Tissues: A Narrative Review

Research RF Safe Research Library Jan 1, 2025

This narrative review explores potential links between EMF exposure, metallic or mixed-metal dental restorations, and reported systemic and neurological symptoms despite normal diagnostic findings. It discusses hypothesized quantum-biological mechanisms (including spin dynamics and radical-pair mechanisms) that could mediate interactions between EMFs and dental metals. The authors conclude that the complexity of these interactions warrants more rigorous research and emphasize that a possible health-risk link should not be ignored.

Numerical analysis of the thermal effects on adult with brain pacemaker implantation exposed to WIFI antennas

Research RF Safe Research Library Jan 1, 2025

This numerical study modeled RF exposure from WiFi/5G-type antennas near a 3D brain model with implanted brain pacemakers relevant to Parkinson’s disease. SAR and temperature increases were reported to remain below ICNIRP 2020 limits across modeled conditions, with maxima at a 90° antenna-to-brain angle. Despite compliance with SAR/temperature limits, the authors report modeled thermal strain and tissue displacement that could affect postoperative efficacy, leading them to recommend caution and increased distance from phones.

Bioelectricity in Morphogenesis

Research RF Safe Research Library Jan 1, 2025

This narrative review discusses bioelectricity arising from membrane potentials and its role in morphogenesis beyond neural tissues. It reports that evidence supports bioelectric signals influencing embryonic development, tissue repair, and disease-related processes, and summarizes cellular mechanisms for generating and sensing these signals. The authors also highlight that potential health implications from natural and artificial electromagnetic fields warrant further scientific attention.

Numerical Analysis of Human Head Exposure to Electromagnetic Radiation Due to 5G Mobile Phones

Research RF Safe Research Library Jan 1, 2025

This conference paper uses numerical simulations to evaluate near-field exposure and thermal effects in a detailed human head model from a realistic 5G mobile phone operating at 26 GHz. The preliminary modeling suggests moderate, localized temperature increases in superficial tissues. The authors emphasize the need for higher-resolution models, refined tissue segmentation, longer exposure durations, and varied phone placements to better characterize potential impacts.

Standards: Exposure Limits for Brief High Intensity Pulses of Radiofrequency Energy Between 6 and 300 GHz

Research RF Safe Research Library Jan 1, 2025

This standards-focused paper evaluates ICNIRP and IEEE (C95.1-2019) exposure limits for brief, high-intensity pulsed RF-EMF between 6 and 300 GHz, particularly when exposures vary within the 6-minute averaging window. Using numerical and analytical modeling with a one-dimensional thermal tissue model, it reports differences in protection against transient skin heating, with IEEE described as more conservative than ICNIRP. The authors propose an adjustment to pulse fluence limits to improve consistency of protection and note that nonthermal and thermoacoustic effects were not analyzed.

Genotoxic and histopathological effects of 6 GHz radiofrequency electromagnetic radiation on rat liver tissue

Research RF Safe Research Library Jan 1, 2025

This animal experiment exposed adult male rats to 6 GHz RF-EMR (0.065 W/kg) for 4 hours/day over 42 days and compared them with sham controls. The exposed group showed higher comet assay genotoxicity metrics, though not statistically significant, and more prominent liver histopathological changes (e.g., portal inflammation and congestion). The authors conclude that 6 GHz exposure can cause histopathological and DNA-level changes in rat liver tissue under the studied conditions.

Effects of coenzyme Q10 on sperm parameters and pathological changes induced by Wi-Fi waves in the testicular tissue of rats

Research RF Safe Research Library Jan 1, 2025

This animal study exposed rats to Wi‑Fi waves for 7 hours/day for 2 months and assessed sperm parameters, serum testosterone, and testicular/epididymal pathology, with and without coenzyme Q10 (CoQ10). The authors report that Wi‑Fi exposure was linked to worse sperm parameters, lower testosterone, and adverse testicular pathology. CoQ10 supplementation during exposure was reported to mitigate these changes compared with Wi‑Fi exposure alone.

13.56 MHz RFID Module - From Application to Process Modelling and Effects on Human Health

Research RF Safe Research Library Jan 1, 2025

This paper presents an application and numerical process modelling of a 13.56 MHz RFID module, including how nearby tags/cards and their positioning affect antenna characteristics. It also considers RFID operation near human tissues and discusses potential health impacts from prolonged EMF exposure at 13.56 MHz. The authors emphasize the importance of evaluating long-term exposure risks and call for additional scientific attention.

Histomorphometric study of thyroid tissue in juvenile rats exposed to 5G electromagnetic fields

Research RF Safe Research Library Jan 1, 2025

This animal study examined thyroid histomorphometry in juvenile male Wistar rats after 2 weeks of 5G EMF exposure (3.5 GHz, 1.5 V/m). Exposed rats showed larger follicle and colloid areas and a significantly lower Thyroid Activation Index, which the authors interpret as thyroid hypoactivity. The authors suggest this may represent a potential health risk and call for further work including hormone assays and mechanistic studies.

Protective effects of quercetin against 3.5 GHz RF radiation-induced thyroid dysfunction and oxidative stress in rats

Research RF Safe Research Library Jan 1, 2025

This rat study examined repeated 3.5 GHz RF exposure (2 hours/day, 5 days/week for 30 days) and thyroid-related outcomes, with and without quercetin. The abstract reports altered thyroid hormones (lower T3/T4, higher TSH) and increased oxidative stress in thyroid tissue after RF exposure. Quercetin appeared partially protective, though effects were not uniformly statistically significant, and SAR simulations indicated relatively higher absorption in the thyroid region.

Electromagnetic fields regulate iron metabolism: From mechanisms to applications

Research RF Safe Research Library Jan 1, 2025

This review synthesizes evidence that electromagnetic field (EMF) exposure is associated in the literature with changes in systemic and cellular iron metabolism, with reported effects varying by EMF parameters, exposure duration, and biological context. It proposes mechanistic pathways involving iron-containing proteins/tissues, membrane and ion channel modulation, and reactive oxygen species (ROS). The authors frame iron-metabolism modulation as relevant to both therapeutic applications and safety evaluation, while emphasizing inconsistencies and the need for standardized exposure protocols.

Melatonin ameliorates RF-EMR-induced reproductive damage by inhibiting ferroptosis through Nrf2 pathway activation

Research RF Safe Research Library Jan 1, 2025

This animal study reports that prolonged RF-EMR exposure (2.45 GHz for 8 weeks) increased oxidative stress and ferroptosis in mouse testicular tissue and was associated with reduced sperm quality. Melatonin administration reportedly mitigated oxidative injury and inhibited ferroptosis. The abstract attributes the protective effect to Nrf2 pathway activation via MT1/MT2 receptors.

Possible effects of RF EMR on contextual fear conditioning, hippocampal perivascular space, apoptosis and adrenal gland microarchitecture in rats

Research RF Safe Research Library Jan 1, 2025

This animal study exposed male Wistar rats to 900 MHz RF-EMR from a mobile phone for 1 hour daily over four weeks and assessed behavior and tissue changes. The authors report altered contextual fear conditioning-related behavior in exposed rats. Histological assessments indicated apoptosis and enlarged perivascular space in the hippocampal CA3 region and apoptotic/inflammatory-like changes in the adrenal zona fasciculata, with no reported differences in adrenal medulla cytoarchitecture.

5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis

Research RF Safe Research Library Jan 1, 2025

This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while UCP1-dependent thermogenesis markers were not significantly changed at the transcriptional level. The authors interpret these findings as a potential partial disruption of brown adipocyte differentiation and raise EMF safety concerns, while calling for further confirmatory research.

Role of radio-frequency electromagnetic waves in causing oxidative stress

Research RF Safe Research Library Jan 1, 2024

This animal study exposed fertilized chick eggs to a nearby 1800 MHz mobile phone that was called repeatedly (50 minutes/day) and assessed embryos at days 10 and 15. The exposed group reportedly showed mitochondrial abnormalities in liver, brain, and heart tissues on electron microscopy, along with increased HSP70 in cardiomyocytes and hepatocytes. The authors conclude that radio-frequency electromagnetic waves can induce oxidative stress and mitochondrial damage in developing embryos.

Mitigating Heat-Induced Sperm Damage and Testicular Tissue Abnormalities: The Protective Role of Radiofrequency Radiation from Wi-Fi Routers in Rodent Models

Research RF Safe Research Library Jan 1, 2024

This rodent study examined whether 2.45 GHz Wi‑Fi router RF-EMF exposure could mitigate heat-stress-related reproductive damage in male rats. The combined RF-EMF + heat group reportedly had improved testicular structure measures and sperm quality versus heat-only, while RF-EMF alone was also reported to alter testis and sperm parameters. The authors interpret the findings as potentially consistent with an adaptive response and call for more research on mechanisms and safety.

Evaluation of the Thyroids of Offsprings Exposed to 2450 MHz Radiofrequency Radiation During Pregnancy: A Sixth Month Data

Research RF Safe Research Library Jan 1, 2024

This animal study examined whether continuous prenatal exposure to 2450 MHz radiofrequency radiation (simulating Wi‑Fi) affects thyroid tissue in rat offspring at 6 months. The exposed group showed significantly increased mononuclear cell infiltration and vascular congestion in thyroid histology. TUNEL-positive cell percentage and H2A.X antibody levels did not differ significantly between groups.

Effect of Exposure to 900 MHz GSM Mobile Phone Radiofrequency Radiation on Estrogen Receptor Methylation Status in Colon Cells of Male Sprague Dawley Rats

Research RF Safe Research Library Mar 1, 2017

This animal study exposed male Sprague-Dawley rats to 900 MHz GSM mobile phone RF radiation for 4 hours and assessed ERα promoter methylation in colon tissue. The authors report altered ERα methylation patterns versus controls after RF exposure. They also report no radioadaptive response in ERα methylation following a subsequent 3 Gy Co-60 gamma challenge.

Biological effects of extremely low frequency electric and magnetic fields: a review

Research RF Safe Research Library Jan 1, 1977

This review summarizes reported biological effects of extremely low frequency (ELF) electric and magnetic fields, describing them as significant and often acting as stressors. Reported outcomes include metabolic, hormonal, and body weight changes in rodents, lethality at high exposure levels in mice and insects, and increased mitotic index in mouse tissues/cells under specified exposure conditions. The review suggests many effects may be mediated through neuroendocrine, nervous system, or behavioral responses to field exposure.

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