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The In Vitro Enhancement of Retinal Cell Viability via mA and mC RNA Methylation-Mediated Changes in the Levels of Heme Oxygenase (HO-1) and DNA Damage Repair Molecules Using a 50 Hz Sinusoidal Electromagnetic Field (EMF).

Research RF Safe Research Library Jan 1, 2024

This in vitro study tested a 50 Hz, 1.3 mT sinusoidal electromagnetic field applied for 15 or 30 minutes to ARPE-19 retinal pigment epithelial cells and RGC-5 retinal ganglion cells. The authors report improved proliferative and migratory capacity after EMF exposure, alongside increased HO-1 and altered RNA…

The extremely low-frequency electromagnetic field (50 Hz) can establish a new "set-point" for the activity of the locus coeruleus-noradrenergic (LC-NA) system in rat.

Research RF Safe Research Library Jan 1, 2024

This animal study exposed rats to 50 Hz ELF-EMF at 1 mT or 7 mT for 1 hour/day over 7 days, repeated across three cycles, and assessed catecholamines, MHPG, β2-adrenergic receptor expression, and behavior. The authors report that ELF-EMF influences stress-response mechanisms involving the locus…

Impact of Extremely Low-Frequency Electromagnetic Fields on Skeletal Muscle of Sedentary Adult Mice: A Pilot Study.

Research RF Safe Research Library Jan 1, 2024

This pilot animal study examined ELF-EMF exposure (0.1 or 1.0 mT, 1 h/day up to 5 weeks) in sedentary adult male C57BL/6 mice, with or without N-acetyl-cysteine (NAC) supplementation. The authors report a transient increase in muscle strength after 2 weeks at 1.0 mT, accompanied by higher PAX7 and myosin heavy chain…

Electromagnetic field as a possible inhibitor of tumor invasion by declining E-cadherin/N-cadherin switching in triple negative breast cancer.

Research RF Safe Research Library Jan 1, 2024

This in vitro study exposed breast cell lines to a 1 Hz, 100 mT ELF-EMF for 5 days (2 h/day) and assessed migration, invasion, and cadherin expression. The authors report reduced migration and invasion in MDA-MB231 cells versus sham exposure. They also report increased E-cadherin and decreased N-cadherin in MDA-MB231…

Extremely low-frequency electromagnetic field (ELF-EMF) induced alterations in gene expression and cytokine secretion in clear cell renal carcinoma cells.

Research RF Safe Research Library Jan 1, 2024

This in vitro study exposed three ccRCC cell lines and a healthy kidney cell line (HEK293) to 50 Hz ELF-EMF at 4.5 mT for 30 minutes daily over 5 days. The authors report exposure-associated changes in expression of several genes (names not fully visible in the abstract), with differences between primary…

Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro.

Research RF Safe Research Library Jan 1, 2023

This in vitro study tested whether ELF-EMF exposure (50 Hz, 1 mT; 30 min/day) influences osteogenic differentiation markers in rat bone-marrow-derived stem cells cultured in osteogenic medium or in human adipose-stem cell-conditioned medium. The OM + EMF condition showed higher ALP activity and higher Runx2 and Ocn…

CaATPase enzyme activities and lipid and protein oxidations of grass carp (Ctenopharyngodon idellus) stored at 4 °C for 30 min under electromagnetic fields.

Research RF Safe Research Library Jan 1, 2023

This study evaluated 12 mT electromagnetic fields (100–400 Hz) applied during 30 minutes of cold storage (4 °C) of grass carp. EMF exposure was associated with increased CaATPase activity and frequency-dependent changes in protein and lipid oxidation. NMR results suggested altered water–protein interactions, and the…

Alternating current electromagnetic field exposure lessens intramyocellular lipid accumulation due to high-fat feeding via enhanced lipid metabolism in mice.

Research RF Safe Research Library Jan 1, 2023

This mouse study tested whether alternating current electromagnetic field (EMF) exposure modifies muscle lipid accumulation during long-term high-fat feeding. Mice exposed to EMF (8 hours/day for 16 weeks; maximum 180 mT) while on a high-fat diet had lower intramyocellular lipid levels than high-fat diet controls.…

The Effect of Low-Frequency Pulsed Electromagnetic Fields on the Differentiation of Permanent Dental Pulp Stem Cells into Odontoblasts.

Research RF Safe Research Library Jan 1, 2023

This in vitro study exposed dental pulp mesenchymal stem cells to 50 Hz pulsed electromagnetic fields at 0.5 or 1 mT for 20 or 40 minutes per day over seven days. Cell survival/proliferation was generally higher than control across exposure groups, except for the 40 minutes/day at 1 mT condition. DMP1 expression was…

Human osteosarcoma cells in response to ELF-MF: Morphological remodeling compared to cell proliferation.

Research RF Safe Research Library Jan 1, 2023

This in vitro study exposed human osteosarcoma (MG-63) cells to 2 mT extremely low-frequency magnetic fields with sinusoidal, triangular, or pulsed waveforms under continuous (3 h) or fractionated (3 days) conditions. The authors report significant decreases in proliferation and shape index in exposed cells versus…

Phagocytosis of latex beads by a human monocytic Mono Mac 6 cell line and effects of low-frequency electromagnetic field interaction.

Research RF Safe Research Library Jan 1, 2023

This in vitro study tested whether a low-frequency EMF (7 Hz, 30 mT) alters phagocytosis-related outcomes in the human monocytic Mono Mac 6 cell line. EMF exposure was associated with increased necrosis in cells pre-stimulated with SEB and then undergoing latex bead phagocytosis, while no significant cell-death…

Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Improves Neurological Outcome and Reduces Microglial Reactivity in a Rodent Model of Global Transient Stroke.

Research RF Safe Research Library Jan 1, 2023

This animal and laboratory study evaluated extremely low-frequency electromagnetic stimulation (ELF-EMS) in a rodent ischemia-reperfusion stroke model and related in vitro/slice experiments. The authors report improved neurological and behavioral outcomes, increased neuronal survival, and reduced glial/microglial…

Synergistic cytotoxic effects of an extremely low-frequency electromagnetic field with doxorubicin on MCF-7 cell line.

Research RF Safe Research Library Jan 1, 2023

This in vitro study tested whether a 50 Hz, 20 mT extremely low-frequency electromagnetic field (ELF-EMF) enhances the cytotoxic effects of doxorubicin (DOX) in the MCF-7 breast cancer cell line. The authors report that combining ELF-EMF with low-dose DOX reduced cell viability at lower DOX concentrations than DOX…

Optimization of cacao beans fermentation by native species and electromagnetic fields.

Research RF Safe Research Library Jan 1, 2023

This study optimized cacao bean fermentation for the CCN 51 clone using native microbial species and applied magnetic fields, varying field density, exposure time, and inoculum concentration. It reports improved yield and bean quality versus a no-magnetic-field control at an optimized condition (5 mT, 22.5 min, 1.6%…

Effect of extremely low frequency electromagnetic field on the pathogenicity of Magnaporthe oryzae.

Research RF Safe Research Library Jan 1, 2023

This laboratory study examined Magnaporthe oryzae pathogenicity under extremely low frequency electromagnetic field exposure (50 Hz, 3 mT). The authors report increased infection ability, growth rate, and conidia generation with ELF-EMF exposure. They also report increased tolerance to a cell wall-perturbing agent…

Effects of repeated exposure to 50 Hz electromagnetic field on breast cancer cells.

Research RF Safe Research Library Jan 1, 2022

This in vitro study exposed two human breast cancer cell lines to a 50 Hz ELF-EMF (20 mT) for 3 hours/day for up to four days. The authors report reduced viability and apoptosis-related findings in MDA-MB-231 cells, with no significant viability change in MCF-7 cells. Cell-cycle effects differed by cell line,…

The Effect of Time-Dependence of 10 Hz Electromagnetic Field on Spatial Learning and Memory in Rats.

Research RF Safe Research Library Jan 1, 2022

This animal study exposed 40 adult male rats to a 10 Hz (4 mT) extremely low-frequency electromagnetic field for 1 hour daily for either 7 or 30 days. The authors report improved spatial memory after prolonged (30-day) exposure, alongside increased hippocampal BDNF levels. They also note a significant body weight…

Effects of prolonged exposure to ELF-EMF on HERVs expression in human melanoma cells.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined whether 96-hour exposure to 50 Hz ELF-EMF affects expression of human endogenous retroviruses (HERV-H, HERV-K, HERV-W) in SK-MEL-37 human melanoma cells. Cells were continuously exposed at 1.5 or 3 mT and HERV env mRNA levels were measured by qPCR. The authors report significant…

High-specificity protection against radiation-induced bone loss by a pulsed electromagnetic field.

Research RF Safe Research Library Jan 1, 2022

This study reports a noninvasive pulsed-burst electromagnetic field (PEMF) approach intended to protect against radiotherapy-induced bone loss. A 15 Hz, 2 mT PEMF waveform is described as producing distinctive calcium oscillations in osteoblasts and inhibiting radiation-related bone loss while not affecting other…

Static and Electromagnetic Fields Differently Affect Proliferation and Cell Death Through Acid Enhancement of ROS Generation in Mesenchymal Stem Cells.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined how a 20 mT static magnetic field and a 20 mT 50 Hz electromagnetic field affect mesenchymal stem cells under conditions involving ROS and acidic pH. The authors report increased ROS and antioxidant-response markers (Nrf2, SOD2, GST) across SMF, EMF, and combined exposures, with…

Protein expression changes during phagocytosis influenced by low-frequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined whether a low-frequency EMF (30 mT, 7 Hz for 3 h) alters phagocytosis of latex beads and immune-related protein/gene expression in the human Mono Mac 6 macrophage cell line. The authors report that EMF exposure influenced phagocytosis-associated molecular markers, with notable changes in…

Bidirectional Effect of Repeated Exposure to Extremely Low-Frequency Electromagnetic Field (50 Hz) of 1 and 7 mT on Oxidative/Antioxidative Status in Rat's Brain: The Prediction for the Vulnerability to Diseases.

Research RF Safe Research Library Jan 1, 2022

This rat study examined repeated 50 Hz ELF-EMF exposure (1 or 7 mT) and measured oxidative stress and antioxidant defense markers in the prefrontal cortex, including responses to a subsequent open-field stressor. The authors report intensity- and repetition-dependent effects: 1 mT produced weak changes and was…

Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined how low-frequency electromagnetic fields (LF-EMF) at different magnetic flux densities influence skeletal myogenesis in C2C12 cells. The authors report that 2.0 mT LF-EMF activated cells and increased PAX7, while 1.5 mT increased MyoD and myogenin expression. They conclude that different…

Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…

Pulsed and Discontinuous Electromagnetic Field Exposure Decreases Temozolomide Resistance in Glioblastoma by Modulating the Expression of O-Methylguanine-DNA Methyltransferase, Cyclin-D1, and p53.

Research RF Safe Research Library Jan 1, 2021

This in vitro study evaluated 50 Hz, 70 G electromagnetic field exposure in combination with temozolomide (TMZ) in T98 and A172 glioblastoma cell lines. The authors report that EMF plus TMZ increased cell death and reduced migration potential compared with controls. They also report increased p53 gene expression and…

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