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Showing results for: extremely low-frequency electromagnetic field

Comparative Study of Extremely Low-Frequency Electromagnetic Field, Radiation, and Temozolomide Administration in Spheroid and Monolayer Forms of the Glioblastoma Cell Line (T98).

Research RF Safe Research Library Jan 1, 2023

This in vitro study assessed 50 Hz, 100 G extremely low-frequency EMF in combination with temozolomide and radiation in T98 glioblastoma cells grown as monolayers and spheroids. Radiation plus EMF increased apoptosis-related gene expression, while temozolomide plus EMF increased autophagy marker expression compared…

The Effect of Time-Dependence of 10 Hz Electromagnetic Field on Spatial Learning and Memory in Rats.

Research RF Safe Research Library Jan 1, 2022

This animal study exposed 40 adult male rats to a 10 Hz (4 mT) extremely low-frequency electromagnetic field for 1 hour daily for either 7 or 30 days. The authors report improved spatial memory after prolonged (30-day) exposure, alongside increased hippocampal BDNF levels. They also note a significant body weight…

The Effect of 12 Hz Extremely Low-frequency Electromagnetic Field on Visual Memory of Male Macaque Monkeys.

Research RF Safe Research Library Jan 1, 2022

This animal experiment assessed visual memory, cortisol, GR gene expression, and amygdala volume in four male rhesus macaques exposed to a 12 Hz, 0.7 μT ELF magnetic field. The exposed monkeys reportedly showed improved visual memory and reduced serum cortisol and GR gene expression, while controls did not show…

Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering.

Research RF Safe Research Library Jan 1, 2022

This in vitro study examined intermittent extremely low-frequency electromagnetic field (ELF-EMF) exposure (6 h/day) in human periodontal ligament mesenchymal stem cells during osteogenic differentiation. After 10 days, ELF-EMF-exposed cells showed increased proliferation and calcium deposition versus sham.…

Bidirectional Effect of Repeated Exposure to Extremely Low-Frequency Electromagnetic Field (50 Hz) of 1 and 7 mT on Oxidative/Antioxidative Status in Rat's Brain: The Prediction for the Vulnerability to Diseases.

Research RF Safe Research Library Jan 1, 2022

This rat study examined repeated 50 Hz ELF-EMF exposure (1 or 7 mT) and measured oxidative stress and antioxidant defense markers in the prefrontal cortex, including responses to a subsequent open-field stressor. The authors report intensity- and repetition-dependent effects: 1 mT produced weak changes and was…

Extremely Low-Frequency Electromagnetic Fields Increase Cytokines in Human Hair Follicles through Wnt/β-Catenin Signaling.

Research RF Safe Research Library Jan 1, 2022

This in vitro/ex vivo study examined extremely low-frequency EMF exposure in human hair bulb spheroids and hair follicle organ cultures. The authors report no toxicity and increased expression of anagen-related markers and multiple cytokines/growth factors at 5–20 G, with 10 G for 60 minutes associated with increased…

Physiotherapeutic Reduction of Orofacial Pain Using Extremely Low-Frequency Electromagnetic Field and Light-Emitting Diode Therapy-A Pilot Study.

Research RF Safe Research Library Jan 1, 2022

This randomized pilot study evaluated whether adding combined extremely low-frequency electromagnetic field (ELF EMF) and LED physiotherapy to standard analgesics reduces postoperative orofacial pain after orthognathic surgery. The physiotherapy group reportedly experienced faster pain reduction than controls. The…

Conformational changes of β-thalassemia major hemoglobin and oxidative status of plasma after in vitro exposure to extremely low-frequency electromagnetic fields: An artificial neural network analysis.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed blood samples from β-thalassemia major patients and matched healthy donors (n=12 each) to 50 Hz ELF-EMF at 0.5 and 1 mT for 1–2 hours. The authors report that two-hour exposure induced significant oxidative changes in β-thalassemia major samples and examined protein/Hb structural features…

Comparing the Effects of Long-term Exposure to Extremely Low-frequency Electromagnetic Fields With Different Values on Learning, Memory, Anxiety, and β-amyloid Deposition in Adult Rats.

Research RF Safe Research Library Jan 1, 2021

This animal study exposed adult male rats to 50 Hz ELF-EMF at 1, 100, 500, or 2000 μT for 1 hour/day over two months and assessed behavior and brain markers. The authors report increased memory retention (higher step-through latency) alongside increased anxiety-like behavior (reduced open-arm entries), particularly…

EVALUATION OF BCL2 AND ITS REGULATORY MIRS, MIR-15-B AND MIR-16 EXPRESSION CHANGES UNDER THE EXPOSURE OF EXTREMELY LOW-FREQUENCY ELECTROMAGNETIC FIELDS ON HUMAN GASTRIC CANCER CELL LINE.

Research RF Safe Research Library Jan 1, 2021

This in vitro study examined whether extremely low-frequency electromagnetic fields (ELF-EMF) at 0.2 or 2 mT (continuous or 1.5 h on/1.5 h off) for 18 hours alter viability and gene/miRNA expression in AGS gastric cancer cells. The authors report a significant reduction in cell viability at 2 mT continuous exposure.…

Up-regulation of miR-144 and miR-375 in the human gastric cancer cell line following the exposure to extremely low-frequency electromagnetic fields.

Research RF Safe Research Library Jan 1, 2021

This in vitro study exposed AGS human gastric cancer cells to 50 Hz ELF magnetic fields at 0.2 or 2 mT for 18 hours (continuous or intermittent). The authors report reduced cell viability versus control and up-regulation of miR-144 and miR-375 during exposure. They also report miR levels decreased 18 and 36 hours…

Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.

Research RF Safe Research Library Jan 1, 2021

This in vitro study tested 50 Hz, 1 mT ELF-EMF exposure (8 h/day for 21 days) and betaine (10 mM) during osteogenic induction of human adipose-derived mesenchymal stem cells. The authors report ELF-EMF decreased osteogenic differentiation and reduced RUNX2 and OCN gene expression compared with non-EMF conditions.…

Enhancement in the ATP level and antioxidant capacity of under continuous exposure to extremely low-frequency electromagnetic field for multiple generations.

Research RF Safe Research Library Jan 1, 2020

This multi-generation animal study exposed worms continuously to 50 Hz, 3 mT extremely low-frequency electromagnetic fields and assessed outcomes in the 15th generation. The study reports increased body length, higher ATP content and ATP synthase activity, and upregulated ATP-related gene expression in exposed worms.…

Electromagnetic field affects the voltage-dependent potassium channel Kv1.3.

Research RF Safe Research Library Jan 1, 2020

This in vitro study tested whether extremely low-frequency electromagnetic fields (ELF-EMF) modulate the voltage-gated potassium channel Kv1.3 in Kv1.3-expressing CHO-K1 cells. Cells were exposed to a 20 Hz field at 268 µT or 902 µT while Kv1.3 currents were measured by whole-cell patch clamp. The authors report a…

Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats.

Research RF Safe Research Library Jan 1, 2020

This rat study examined chronic extremely low-frequency electromagnetic field (ELF-EMF) exposure and diaphragm muscle function and structure. Rats were exposed to 50 Hz, 1.5 mT for 4 hours daily for up to 7 months and assessed with electrophysiological, biochemical, and electron-microscopy measures. The authors…

The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells.

Research RF Safe Research Library Jan 1, 2020

This in vitro study tested whether 50 Hz extremely low-frequency electromagnetic fields (0.5 or 1 mT) affect human dental pulp stem cell survival and proliferation. Using MTT and DAPI assays after 7 days of exposure (20 or 40 min/day), all exposed groups reportedly showed higher viability/proliferation than controls.…

Immune response of mollusk Onchidium struma to extremely low-frequency electromagnetic fields (ELF-EMF, 50 Hz) exposure based on immune-related enzyme activity and De novo transcriptome analysis.

Research RF Safe Research Library Jan 1, 2020

This animal study exposed the mollusk Onchidium struma to 50 Hz ELF-EMF at 100 μT or 500 μT for up to one week and assessed immune-related cell counts, enzyme activities, and transcriptomic changes. The 100 μT group showed increased total coelomocyte and spherulocyte density versus control and 500 μT, while some cell…

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.

Research RF Safe Research Library Jan 1, 2020

This in vitro study exposed human dermal papilla cells to 70 Hz extremely low-frequency electromagnetic fields at 5–100 G for four days. The abstract reports increased expression of several anagen-related molecules and increased proliferation, with 10 G described as the most potent condition. It also reports…

Lipidomic alteration and stress-defense mechanism of soil nematode Caenorhabditis elegans in response to extremely low-frequency electromagnetic field exposure.

Research RF Safe Research Library Jan 1, 2019

This animal (nematode) study exposed Caenorhabditis elegans to 50 Hz, 3 mT extremely low-frequency EMF and assessed multi-omics responses. The authors report significant lipidomic, proteomic, and transcriptomic alterations, including increased triacylglycerols. Pathway analyses suggest disrupted lipid metabolism,…

T-Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low-Frequency Electromagnetic Fields Via Induction of IL-2 Signaling.

Research RF Safe Research Library Jan 1, 2019

This in vitro study examined 60 Hz extremely low-frequency EMF exposure (0.8 mT) on CD4 T cells under Th9-polarizing conditions. The authors report increased Th9-associated responses including IL-9 secretion, transcription factor changes (including early GATA3), and increased STAT5/STAT6 phosphorylation alongside…

Impact of extremely low-frequency electromagnetic field (100 Hz, 100 G) exposure on human glioblastoma U87 cells during Temozolomide administration.

Research RF Safe Research Library Jan 1, 2019

This in vitro study evaluated whether extremely low-frequency EMF exposure (100 Hz, 100 G) modifies the effects of temozolomide (100 µM) in human glioblastoma U87 cells over 120–144 hours. The authors report that combined treatment reduced stemness-associated markers (Nestin, CD133, Notch4) and increased GFAP…

Correlation between the Lunar Phase and Tail-Lifting Behavior of Lizards () Exposed to an Extremely Low-Frequency Electromagnetic Field.

Research RF Safe Research Library Jan 1, 2019

This animal study examined long-term tail-lifting behavior in agamid lizards exposed to an ELF electromagnetic field (6 and 8 Hz; peak magnetic field 2.6 µT; peak electric field 10 V/m) versus controls, incorporating lunar phase and geomagnetic activity (K index) as explanatory variables. Regression analyses found…

Geobacter sulfurreducens-inoculated bioelectrochemical system reveals the potential of metabolic current in defining the effect of extremely low-frequency electromagnetic field on living cells.

Research RF Safe Research Library Jan 1, 2019

This laboratory study exposed a Geobacter sulfurreducens-inoculated bioelectrochemical system to 0–128 Gauss extremely low-frequency electromagnetic fields. The authors report increased and oscillatory metabolic current under ELF-EMF exposure, with dose-dependent effects and a linear relationship between oscillation…

Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis.

Research RF Safe Research Library Jan 1, 2018

This in vitro study used a 3D dermal papilla-like tissue model containing dermal papilla cells and melanocytes to assess melanogenesis after rice bran extract (RBE), extremely low-frequency EMF, or their combination. The combined RBE/EMF condition showed higher tyrosinase activity, increased melanogenesis-related…

Increase in Blood Levels of Growth Factors Involved in the Neuroplasticity Process by Using an Extremely Low Frequency Electromagnetic Field in Post-stroke Patients.

Research RF Safe Research Library Jan 1, 2018

This study evaluated whether adding extremely low-frequency electromagnetic field (ELF-EMF) therapy to standard post-stroke rehabilitation affects biomarkers of neuroplasticity and functional recovery. Forty-eight patients completed the same rehabilitation program, with the study group additionally receiving 10…

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