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A Prolonged exposure to Wi-Fi Radiation Induces Neurobehavioral Changes and Oxidative Stress in Adult Zebrafish

Research RF Safe Research Library Jan 1, 2025

This animal study exposed adult zebrafish to 2.45 GHz Wi‑Fi radiation for 4 hours daily over 30 consecutive days. The authors report neurobehavioral impairments with altered locomotion, alongside decreased acetylcholinesterase and increased brain oxidative stress. They conclude these findings indicate a safety risk and call for further mechanistic and public health research.

Investigation of the Effects of 2.45 GHz Near-Field EMF on Yeast

Research RF Safe Research Library Jan 1, 2025

This in vitro study exposed yeast suspensions to 2.45 GHz near-field microwave radiation at 2 cm and 4 cm for 20 or 60 minutes. It reports oxidative-stress-related changes (reduced antioxidant activity with increased membrane permeability) after 20 minutes at 2 cm, an effect not reproduced by conventional heating. The study also reports a trend toward increased DNA damage under both exposure conditions and mild membrane permeability changes after 60 minutes at 4 cm.

Low frequency magnetic field exposure and neurodegenerative disease: systematic review of animal studies

Research RF Safe Research Library Jan 1, 2025

This systematic review synthesizes animal studies on low frequency magnetic field exposure in relation to neurodegenerative diseases. It reports no support for a causal induction of Alzheimer’s-type neuropathology in naive animals, while noting that evidence is too limited to draw strong conclusions for motor neuron disease, multiple sclerosis, and Parkinson’s disease regarding induced neuropathology. In models with pre-existing neurodegenerative disease, the review describes possible therapeutic effects on behavioral and neuroanatomical outcomes for dementia-related conditions, and no apparent effect on motor neuron disease progression.

Proteomic Characterization of Human Peripheral Blood Mononuclear Cells Exposed to a 50 Hz Magnetic Field

Research RF Safe Research Library Jan 1, 2025

This in vitro study compared proteomic profiles of PBMCs from three human donors after 24-hour exposure to a 50 Hz, 1 mT extremely low-frequency magnetic field versus unexposed cells. The abstract reports broad protein expression changes, including upregulation of proteins associated with metabolic processes and downregulation of proteins linked to T cell costimulation/activation and immune processes. No effects were observed on cell proliferation, viability, or cell cycle progression. The authors interpret the proteomic shifts as metabolic reprogramming with potential implications for immune regulation.

Microleakage of Amalgam Restorations after Exposure to Electromagnetic Fields of a Commercial Hair Dryer: An Ex-Vivo Study

Research RF Safe Research Library Jan 1, 2025

This ex-vivo experimental study tested whether electromagnetic fields from a commercial hair dryer affect microleakage of class V dental amalgam restorations in 100 extracted human teeth. Several exposure groups showed significantly higher dye-penetration microleakage scores than the unexposed control, while one exposure condition did not differ from control. The authors conclude that hair-dryer EMF exposure can increase microleakage and potentially compromise restoration integrity.

Radiofrequency electromagnetic fields reduce bumble bee visitation to flowers

Research RF Safe Research Library Jan 1, 2025

This blinded, two-year study examined whether RF-EMF exposure at 2.4 and 5.8 GHz affects pollinator visitation to Salvia and Lavandula. The authors report no significant effect on honey bee visitation rates. They report a significant reduction in bumble bee visits per observation period under RF-EMF exposure, which they frame as a potential risk warranting further long-term research.

Perspectives on terahertz honeybee sensing

Research RF Safe Research Library Jan 1, 2025

This paper describes measurements and simulations to support terahertz (THz) sensing of European honey bees for environmental monitoring. It reports dielectric characterization of bee body parts across 1–500 GHz, scattering-based validation of 3D-printed bee mockups, and THz imaging demonstrating detailed anatomical visualization. The work includes dosimetric simulations at 300 GHz to evaluate feasibility of non-invasive, continuous monitoring and notes potential relevance to assessing high-frequency EMF impacts on insect health and habitat safety.

13.56 MHz RFID Module - From Application to Process Modelling and Effects on Human Health

Research RF Safe Research Library Jan 1, 2025

This paper presents an application and numerical process modelling of a 13.56 MHz RFID module, including how nearby tags/cards and their positioning affect antenna characteristics. It also considers RFID operation near human tissues and discusses potential health impacts from prolonged EMF exposure at 13.56 MHz. The authors emphasize the importance of evaluating long-term exposure risks and call for additional scientific attention.

Greater prevalence of symptoms associated with higher exposures to mobile phone base stations in a hilly, densely populated city in Mizoram, India

Research RF Safe Research Library Jan 1, 2025

This cross-sectional study compared 183 higher-exposed residents with 126 matched reference residents and assessed symptoms via questionnaire alongside in-home RF-EMF power density measurements from mobile phone base stations. Higher exposure (including proximity within 50 m and power densities of 5–8 mW/m2) was reported to be associated with increased symptom prevalence across mood-energy, cognitive-sensory, inflammatory, and anatomical categories. The authors conclude that current public exposure limits may be inadequate for long-term, non-thermal biological impacts and call for precautionary policy updates.

Trends in Malignant and Benign Brain Tumor Incidence and Mobile Phone Use in the U.S. (2000-2021): A SEER-Based Study

Research RF Safe Research Library Jan 1, 2025

This SEER-based ecological study examined U.S. trends (2000–2021) in malignant and benign brain tumor incidence and compared them with national mobile phone subscription trends. Malignant brain tumor incidence in adolescents and adults declined slightly, while benign brain tumor incidence increased over time; temporal lobe tumors and benign acoustic neuromas showed little change. The authors interpret these patterns as not supporting an association between mobile phone use and increased brain cancer risk, while recommending continued surveillance given rising benign tumor incidence and potential latency.

5G Radio-Frequency-Electromagnetic-Field Effects on the Human Sleep Electroencephalogram: A Randomized Controlled Study in CACNA1C Genotyped Volunteers

Research RF Safe Research Library Jan 1, 2025

This randomized, double-blind, sham-controlled study tested whether CACNA1C rs7304986 genotype modifies sleep EEG responses to 5G RF-EMF exposure. The authors report a genotype-by-exposure interaction, with 3.6 GHz exposure in T/C carriers associated with a faster NREM sleep spindle center frequency versus sham. The abstract also notes longer sleep latency in T/C compared with T/T carriers, and concludes that genetically susceptible groups may show differential physiological responses to 5G RF-EMF.

Exposure to radiofrequency electromagnetic fields and IARC carcinogen assessment: Risk of Bias preliminary literature assessment for 10 key characteristics of human carcinogens

Research RF Safe Research Library Jan 1, 2025

This review examined experimental literature on whether RF-EMF exposures within ICNIRP (2020) limits affect IARC key characteristics of human carcinogens. It identified 159 articles and found that 38% of in vitro/in vivo measurements reported statistically significant effects, but higher study quality was associated with fewer reported effects and there was no consistent exposure-response pattern. The authors state that study diversity and generally poor quality prevent high-confidence conclusions for most key characteristics, while recommending replication of the few higher-quality positive findings under stringent standards.

Does Electromagnetic Pollution in the ART Laboratory Affect Sperm Quality? A Cross-Sectional Observational Study.

Research RF Safe Research Library Jan 1, 2025

This cross-sectional observational study assessed sperm motility after one hour of in vitro exposure of semen samples to EMFs from different laboratory sources in an IVF setting. It reports a statistically significant reduction in progressive sperm motility after exposure to mobile phones and Wi-Fi repeaters, while other EMF-emitting equipment showed no significant effect. The authors interpret the findings as indicating a potential negative impact of specific RF sources and call for further research, alongside practical mitigation suggestions in IVF laboratories.

A novel approach for assessments of radiofrequency electromagnetic fields exposure in buildings near telecommunication infrastructure

Research RF Safe Research Library Jan 1, 2025

This paper proposes a new methodology to better assess indoor RF-EMF exposure in buildings near telecommunication base station antennas by refining measurement-point selection. Implemented in four multi-storey buildings in Natal, Brazil, indoor electric field peaks and averages were reported to be substantially higher than ground-level measurements. Although the highest indoor levels remained below ICNIRP recommended limits, the authors argue current regulatory evaluation methods may underestimate indoor exposure in certain building locations.

Effect of elevation on cumulative radiofrequency exposure from multiple communication towers

Research RF Safe Research Library Jan 1, 2025

This exposure assessment measured RF power density across different floors of a high-rise university building using a spectrum analyser and log-periodic antenna. Power density decreased from the ground to the third floor but peaked on the fourth (top) floor. The 900 MHz band showed the highest reported power density (1.16E-03 W/m2), and the authors suggest higher-floor occupants may experience higher RF exposure from nearby communication towers.

Evaluation of Personal Radiation Exposure from Wireless Signals in Indoor and Outdoor Environments

Research RF Safe Research Library Jan 1, 2025

This exposure assessment measured personal RF electric field strength in multiple indoor and outdoor micro-environments in Malaysia using an ExpoM-RF 4 meter and modeled exposure with machine learning (FCNN, XG Boost) and linear regression. Reported exposures were usually below the stated public limit (61.4 V/m), but maximum values in dense urban areas with many base stations were reported to approach 56.7365 V/m. The authors frame near-threshold maxima in high-density areas as a potential health risk and recommend caution and monitoring.

Auto-Induced Downlink Radiofrequency Electromagnetic Field Exposure at 3.5 GHz With Focusing Near the Head

Research RF Safe Research Library Jan 1, 2025

This exposure-assessment study uses FDTD simulations to evaluate auto-induced downlink RF-EMF exposure at 3.5 GHz when downlink energy is focused toward user equipment near the head. Exposure varied substantially by device position (ear, eyes, nose) and by the precoding technique used. The authors report that the choice of normalization strategy can produce cases where ICNIRP basic restrictions are exceeded even when reference levels appear compliant, motivating a precautionary framing for compliance assessment.

In-Situ Measurements of Radiofrequency Electromagnetic Fields Measurements Around 5G Macro Base Stations in the UK

Research RF Safe Research Library Jan 1, 2025

This exposure assessment performed RF spot measurements in line-of-sight to 56 active 5G macro base stations across 30 publicly accessible UK locations. Power density was measured across 420 MHz–6 GHz under multiple scenarios (background, streaming, downlink speed test, and extrapolated SS-RSRP decoding). Reported total RF and 5G-specific levels were within 1998 ICNIRP public reference levels, with 4G downlink contributing most of the measured exposure.

Exposure to 26.5 GHz, 5G modulated and unmodulated signal, does not affect key cellular endpoints of human neuroblastoma cells

Research RF Safe Research Library Jan 1, 2025

This in vitro study examined whether 26.5 GHz millimeter-wave exposure (continuous wave and 5G-modulated) affects key cellular endpoints in human neuroblastoma cells. Cells were exposed for 3 hours at SAR 1.25 W/kg using a reverberation-chamber system, with assessments including cell cycle and DNA damage. The study reports no effects from exposure alone or when combined with the oxidant menadione, while noting that additional studies across varied conditions are needed.

Histomorphometric study of thyroid tissue in juvenile rats exposed to 5G electromagnetic fields

Research RF Safe Research Library Jan 1, 2025

This animal study examined thyroid histomorphometry in juvenile male Wistar rats after 2 weeks of 5G EMF exposure (3.5 GHz, 1.5 V/m). Exposed rats showed larger follicle and colloid areas and a significantly lower Thyroid Activation Index, which the authors interpret as thyroid hypoactivity. The authors suggest this may represent a potential health risk and call for further work including hormone assays and mechanistic studies.

Protective effects of quercetin against 3.5 GHz RF radiation-induced thyroid dysfunction and oxidative stress in rats

Research RF Safe Research Library Jan 1, 2025

This rat study examined repeated 3.5 GHz RF exposure (2 hours/day, 5 days/week for 30 days) and thyroid-related outcomes, with and without quercetin. The abstract reports altered thyroid hormones (lower T3/T4, higher TSH) and increased oxidative stress in thyroid tissue after RF exposure. Quercetin appeared partially protective, though effects were not uniformly statistically significant, and SAR simulations indicated relatively higher absorption in the thyroid region.

The effects of short-term and long-term 2100 MHz radiofrequency radiation on adult rat auditory brainstem response

Research RF Safe Research Library Jan 1, 2025

This animal study examined 2100 MHz radiofrequency radiation exposure effects on auditory brainstem responses and brain oxidative/ultrastructural markers in adult rats. The 1-week exposure group showed prolonged ABR latencies and biochemical/structural changes consistent with oxidative stress and cellular injury. The authors report no harmful effects in the 10-week exposure condition with rest days under the studied protocol.

Electromagnetic fields from mobile phones: A risk for maintaining energy homeostasis?

Research RF Safe Research Library Jan 1, 2025

This narrative review discusses low-intensity RF-EMF exposure, primarily from mobile phones, with a focus on thermoregulation and energy homeostasis. It reports that many rodent studies at 900 MHz describe cold-like thermoregulatory and behavioral responses and molecular findings suggestive of WAT browning, while BAT transcriptional changes typical of cold exposure were not observed. The authors indicate short-term adaptations may not disrupt homeostasis, but emphasize uncertainty about long-term consequences and call for further research, including at 5G-relevant frequencies.

Characterization of the Core Temperature Response of Free-Moving Rats to 1.95 GHz Electromagnetic Fields

Research RF Safe Research Library Jan 1, 2025

This animal study measured core body temperature in free-moving male and female Sprague Dawley rats during and after 3-hour exposure to 1.95 GHz RF-EMF at multiple whole-body average SAR levels. A measurable thermal response was reported at 4 W/kg, while lower SAR conditions showed smaller or no significant temperature increases. The authors note that temperature dropped quickly after exposure ended, implying post-exposure measurements may underestimate peak heating.

The Influence of an Electromagnetic Field at a Radiofrequency of 900 MHz on the Behavior of a Honey Bee

Research RF Safe Research Library Jan 1, 2025

This laboratory study examined whether a 900 MHz radiofrequency electromagnetic field (RF-EMF) influences the behavior of newly emerged honey bee workers. Compared with controls, the exposed groups showed behavioral changes, with some parameters showing statistically significant differences seven days after exposure. The authors frame RF-EMF as a potential environmental stressor and call for further research, including gene expression analyses.

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