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Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

Research RF Safe Research Library Jan 1, 2025

This in vitro study exposed pig conceptuses (days 15–16 of pregnancy) to 50 Hz ELF-EMF for 2 hours and assessed transcriptomic and DNA methylation changes. The authors report altered expression of 21 protein-coding transcripts and an approximately 16-fold increase in genomic DNA methylation, with promoter methylation changes in several named genes. They conclude ELF-EMF interacts with gene expression and DNA methylation processes during early development and call for further safety research.

Potential influence of geomagnetic activity on blood pressure statistical fluctuations at mid-magnetic latitudes

Research RF Safe Research Library Jan 1, 2025

This six-year time series analysis used over 500,000 blood pressure measurements from two mid-magnetic latitude cities in China to examine associations with geomagnetic activity (Ap index). The study reports that blood pressure fluctuations correlate with geomagnetic activity and share similar periodic patterns, unlike air temperature and PM2.5. The authors conclude that high geomagnetic activity periods may increase risk for individuals with hypertension and note potential clinical and policy relevance.

The effects of radiofrequency radiation on male reproductive health and potential mechanisms (Review)

Research RF Safe Research Library Jan 1, 2025

This narrative review summarizes human and animal research on radiofrequency (RF) radiation exposure (e.g., mobile phones, Wi‑Fi, occupational sources) and male reproductive outcomes. It reports that the literature links RF exposure with reduced sperm quality and increased DNA damage, often alongside oxidative stress and other proposed biological changes. Although inconsistencies are acknowledged, the authors conclude the overall evidence suggests harmful associations and call for standardized, long-term studies and reconsideration of guidelines.

Self-diagnosing electromagnetic hypersensitivity-A case study

Research RF Safe Research Library Jan 1, 2025

No abstract was provided in the source text. The provided overview indicates this is a case study of self-diagnosed electromagnetic hypersensitivity (EHS), describing symptoms attributed by an individual to EMF exposure. The supplied text emphasizes ongoing concern about potential health risks and calls for rigorous scientific inquiry and support for affected individuals.

RF-EMF exposure assessment with add-on uplink exposure sensor in different microenvironments in seven European countries

Research RF Safe Research Library Jan 1, 2025

This exposure assessment study introduces a cost-efficient add-on sensor attached to a smartphone to quantify auto-induced uplink RF-EMF transmission across 100–6000 MHz in multiple microenvironments. Activity-based surveys were conducted in seven European countries under non-user, maximum downlink, and maximum uplink scenarios. Reported power levels were lowest for non-user scenarios and higher during active use, with variation by country, urbanization, and setting. The authors frame the work as supporting future epidemiological research and planned validation against other tools.

The Effect of Proximity Sensor & Grip Sensor Use on Specific Absorption Rate (SAR) in Smartphones

Research RF Safe Research Library Jan 1, 2025

This engineering study examined how smartphone proximity and grip sensors affect SAR during LTE and 5G NR operation in a 3D measurement environment. The abstract reports that enabling these sensors reduces SAR relative to being turned off, with reductions varying by sensor and frequency. The authors attribute the reduction to sensor-driven power management and transmission power adjustment.

Repeated Head Exposures to a 5G-3.5 GHz Signal Do Not Alter Behavior but Modify Intracortical Gene Expression in Adult Male Mice

Research RF Safe Research Library Jan 1, 2025

This animal study examined repeated asymmetrical head exposure to a 5G-modulated 3.5 GHz signal in adult male mice for six weeks. It reports no significant changes in locomotion, anxiety, or object-based memory performance under the tested conditions. However, it found statistically significant but limited cortical gene expression changes (<1% of expressed genes), including enrichment for glutamatergic synapse-related genes and lateralized differences involving mitochondrial genome-encoded genes. The authors caution that potential health risks from these intracortical transcriptomic modifications should not be downplayed and note uncertainties about longer exposures and other populations.

5G Radiofrequency Exposure Reduces PRDM16 and C/EBP � mRNA Expression, Two Key Biomarkers for Brown Adipogenesis

Research RF Safe Research Library Jan 1, 2025

This animal study exposed juvenile and young adult Wistar rats to 5G (3.5 GHz) or 2G (900 MHz) radiofrequency fields (1.5 V/m) for 1–2 weeks and measured brown adipose tissue-related gene expression by RT-qPCR. The abstract reports significant downregulation of PRDM16 and C/EBP mRNA after 5G exposure, while UCP1-dependent thermogenesis markers were not significantly changed at the transcriptional level. The authors interpret these findings as a potential partial disruption of brown adipocyte differentiation and raise EMF safety concerns, while calling for further confirmatory research.

The CB1R of mPFC is involved in anxiety-like behavior induced by 0.8/2.65 GHz dual-frequency electromagnetic radiation

Research RF Safe Research Library Jan 1, 2025

This animal study reports that dual-frequency RF EMR exposure (0.8/2.65 GHz, 4 W/kg) induced anxiety-like behavior in mice. It also reports reduced CB1R and endocannabinoid levels in the mPFC and altered endocannabinoid system markers in the BLA. CB1R overexpression or knockdown in the mPFC reportedly decreased or increased anxiety-like behavior, respectively, suggesting a mechanistic link in this model.

Histomorphometry and Sperm Quality in Male Rats Exposed to 2.45 GHz Wi-Fi

Research RF Safe Research Library Jan 1, 2025

This animal study exposed adult male rats to 2.45 GHz Wi‑Fi from an active router for 4 or 24 hours daily over eight weeks and assessed reproductive organ histology and sperm parameters. The authors report histological changes in testes and epididymis, multifocal atypical hyperplasia in seminal vesicles, reduced seminiferous tubule diameter, and reduced spermatogenesis index in exposed groups. Sperm concentration decreased in both exposed groups, motility decreased in the 4-hour group, and viability increased in the 24-hour group, leading to an overall interpretation of potential reproductive risk under the studied conditions.

Extremely low frequency magnetic field distracts zebrafish from a visual cognitive task

Research RF Safe Research Library Jan 1, 2025

This animal study trained adult zebrafish to perform a conditioned avoidance response to a visual cue. The visual cue was presented alone or together with an extremely low frequency sinusoidally changing magnetic field (0.3 Hz) at 0.015 mT or 0.06 mT. The abstract reports that the 0.06 mT magnetic field condition impaired learning performance and response behavior, suggesting a cross-modal distraction effect.

Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells

Research RF Safe Research Library Jan 1, 2025

This in vitro study examined effects of moderate static magnetic field exposure on sympathetic neuron-like PC12 cells. The authors report a significant reduction in voltage-gated potassium (Kv) channel current density after 18 hours of exposure, with the reduction persisting after the field was removed. RNA sequencing identified 37 SMF-sensitive genes and pathway analyses suggested upregulation of signaling associated with reduced neuronal excitability.

Role of radio-frequency electromagnetic waves in causing oxidative stress

Research RF Safe Research Library Jan 1, 2024

This animal study exposed fertilized chick eggs to a nearby 1800 MHz mobile phone that was called repeatedly (50 minutes/day) and assessed embryos at days 10 and 15. The exposed group reportedly showed mitochondrial abnormalities in liver, brain, and heart tissues on electron microscopy, along with increased HSP70 in cardiomyocytes and hepatocytes. The authors conclude that radio-frequency electromagnetic waves can induce oxidative stress and mitochondrial damage in developing embryos.

Temporal Change of Outdoor Rf-Emf Levels Four in European Countries: A Microenvironmental Measurement Study

Research RF Safe Research Library Jan 1, 2024

This microenvironmental measurement study assessed temporal trends in outdoor RF-EMF exposure from 2016 to 2023 in four European countries using repeated walking-route measurements with exposimeters. The abstract reports that data did not suggest significant changes in mobile base station (downlink) exposure over time and that trends were not consistent across individual microenvironments. Reported median downlink exposure values varied by country and year but did not indicate an overall increase despite higher mobile data traffic.

Experimental Study of Animal Behavior under the Influence of the Electromagnetic Field of the 5G Mobile Communication Standard

Research RF Safe Research Library Jan 1, 2024

This pilot animal study exposed mature male Wistar rats to 4.9 GHz electromagnetic fields described as 5G-standard, for 15 days (2 hours/day) in a semi-anechoic shielded chamber. Open-field testing found no clear behavioral differences between unmodulated and modulated exposures. Statistically significant behavioral changes occurred in both exposed and control animals, which the authors attribute to changes in the environmental electromagnetic background, making causal interpretation uncertain.

Effect of Repeated Exposure to Complexly Organized Electromagnetic Radiation on the Rat Behavior in the "Open Field" Test

Research RF Safe Research Library Jan 1, 2024

This animal study examined repeated pulsed-modulated RF exposure (1–4 GHz; total pulse power density 300 μW/cm2) in male and female Wistar rats and assessed behavior using the open field test. The abstract reports stress reactions and long-term memory impairment in some rats, with females described as more sensitive than males. Reported effects were transient, with behavior returning to baseline within 1.5–2 months after exposure stopped. The authors suggest potential concern for constant exposure scenarios, though this is not directly evaluated in humans here.

Experimental Study of Stress Reactions Induced by Low-Intensity Microwave Radiation

Research RF Safe Research Library Jan 1, 2024

This animal experiment examined hematopoietic and immune-related indicators in mice after repeated exposure to low-intensity microwave radiation. Exposure involved monochromatic pulsed fields in the 2.27–2.78 GHz range with average power flux density of 60 μW/cm² and doses of 0.086–0.86 J/g. The authors report cumulative biological effects consistent with a stress-like adaptive reaction, based on changes in bone marrow, spleen CFU-S measures, erythrocyte hemolytic resistance, and thymus metrics.

A Decision Support System for Managing Health Symptoms of Living Near Mobile Phone Base Stations

Research RF Safe Research Library Jan 1, 2024

This analytical study evaluated machine learning models (SVM and Random Forest) to predict health symptoms in adults living near mobile phone base stations. The SVM model reportedly achieved high predictive performance for headache, sleep disturbance, dizziness, vertigo, and fatigue, and outperformed Random Forest and prior models. The abstract concludes that proximity to base stations is connected with increased prevalence of several symptoms and emphasizes distance, age, and duration of residence as key predictors.

Mitigating Heat-Induced Sperm Damage and Testicular Tissue Abnormalities: The Protective Role of Radiofrequency Radiation from Wi-Fi Routers in Rodent Models

Research RF Safe Research Library Jan 1, 2024

This rodent study examined whether 2.45 GHz Wi‑Fi router RF-EMF exposure could mitigate heat-stress-related reproductive damage in male rats. The combined RF-EMF + heat group reportedly had improved testicular structure measures and sperm quality versus heat-only, while RF-EMF alone was also reported to alter testis and sperm parameters. The authors interpret the findings as potentially consistent with an adaptive response and call for more research on mechanisms and safety.

Evaluation of the Thyroids of Offsprings Exposed to 2450 MHz Radiofrequency Radiation During Pregnancy: A Sixth Month Data

Research RF Safe Research Library Jan 1, 2024

This animal study examined whether continuous prenatal exposure to 2450 MHz radiofrequency radiation (simulating Wi‑Fi) affects thyroid tissue in rat offspring at 6 months. The exposed group showed significantly increased mononuclear cell infiltration and vascular congestion in thyroid histology. TUNEL-positive cell percentage and H2A.X antibody levels did not differ significantly between groups.

The effects of radiofrequency exposure on male fertility: A systematic review of human observational studies with dose-response meta-analysis (SR 3)

Research RF Safe Research Library Jan 1, 2024

This systematic review and dose-response meta-analysis synthesizes human observational studies on radiofrequency EMF exposure and male fertility outcomes. It reports evidence of an association between RF exposure and poorer sperm parameters, including reduced quality, motility, and viability. The authors frame the findings as consistent with potential reproductive health risks and call for continued risk assessment and guideline development.

Effects of radiofrequency field from 5G communication on fecal microbiome and metabolome profiles in mice

Research RF Safe Research Library Jan 1, 2024

This animal study exposed adult male C57BL/6 mice to a 4.9 GHz radiofrequency field for three weeks (1 hour/day) and compared them with a sham group. The abstract reports altered fecal microbiome composition with reduced diversity in the RF group, along with 258 significantly differentially abundant fecal metabolites. The authors conclude that 4.9 GHz RF exposure is associated with changes in gut microbiota and metabolic profiles and call for further EMF safety research.

Toxicology and carcinogenesis studies in Sprague Dawley (Hsd:Sprague Dawley SD) rats exposed to whole-body radio frequency radiation at a frequency (900 MHz) and modulations (GSM and CDMA) used by cell phones

Research RF Safe Research Library Jan 1, 2018

This National Toxicology Program technical report describes 900 MHz whole-body RFR exposures (GSM and CDMA) in male and female Sprague Dawley rats from in utero through up to 2 years. The report concludes clear evidence of carcinogenic activity in males for both modulations based on malignant schwannoma of the heart, with malignant glioma of the brain also reported as related to exposure. In females, the report concludes equivocal evidence of carcinogenic activity for both modulations based on selected tumor outcomes, and genetic toxicology findings were mixed with some comet assay increases/equivocal results but negative micronucleus assays.

Electromagnetic Fields Act Similarly in Plants as in Animals: Probable Activation of Calcium Channels via Their Voltage Sensor

Research RF Safe Research Library Jan 1, 2016

This narrative review proposes that low-intensity microwave/lower-frequency EMFs activate plasma membrane calcium channels in animals, increasing intracellular calcium and triggering downstream signaling including oxidative stress pathways. It further suggests that EMF actions in terrestrial multicellular plants are probably similar, with plant two-pore channels proposed as plausible mediators due to a comparable voltage sensor. The abstract describes briefly reviewed plant studies as consistent with this mechanism, but does not provide detailed exposure parameters or quantitative results.

Microwave frequency electromagnetic fields (EMFs) produce widespread neuropsychiatric effects including depression

Research RF Safe Research Library Jan 1, 2016

This 2016 narrative review proposes that non-thermal microwave/lower-frequency EMFs act primarily through activation of voltage-gated calcium channels (VGCCs), with calcium channel blockers reported to block EMF effects. It summarizes animal, occupational, and epidemiological literature and reports that exposures from base stations, heavy mobile phone use, and wireless smart meters are associated with neuropsychiatric symptoms, sometimes with doseresponse patterns. The author concludes that multiple lines of evidence collectively support that non-thermal microwave EMF exposures can produce diverse neuropsychiatric effects including depression.

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